Utility of a set of conserved mitochondrial cytochrome oxidase subunit I gene primers for Mansonia annulata identification

Chalalai Rueanghiran; Chamnarn Apiwathnasorn; Pradit Sangthong; Yudthana Samung; Jiraporn Ruangsittichai

Utility of a set of conserved mitochondrial cytochrome oxidase subunit I gene primers for Mansonia annulata identification

Chalalai Rueanghiran
, Chamnarn Apiwathnasorn
, Pradit Sangthong
, Yudthana Samung
, Jiraporn Ruangsittichai

Department of Medical Entomology

Faculty of Tropical Medicine, Mahidol University
Kasetsart University

Research output: Contribution to journal › Review article › peer-review

6 Citations (Scopus)

Abstract

DNA-based identification system using the mitochondrial cytochrome oxidase subunit I (COI) gene has enabled validation of many species in certain taxonomic groups. These primer combinations were able to work universally across Insecta. Here, a set of three primer pairs were successful in amplifying COI of Mansonia annulata mosquito, a potential vector of Brugia malayi. By merging all three amplicons the whole COI was obtained. Primer pair TY-J-1460/C1N2087 amplified 5' region of COI, LepFl/LepRl the central and C1J2090/TL2N3014 the 3' region, generating COI amplicons of 650, 700 and 950 base pairs, respectively. When Ma. annulata sequences were compared with those from online sources, they formed a cluster group that is clearly distinct from other allied species.

Original language	English
Pages (from-to)	1381-1387
Number of pages	7
Journal	Southeast Asian Journal of Tropical Medicine and Public Health
Volume	42
Issue number	6
Publication status	Published - 2011

Keywords

COI
Mansonia annulata
Mitochondrial DNA
Primer

Cite this

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title = "Utility of a set of conserved mitochondrial cytochrome oxidase subunit I gene primers for Mansonia annulata identification",

abstract = "DNA-based identification system using the mitochondrial cytochrome oxidase subunit I (COI) gene has enabled validation of many species in certain taxonomic groups. These primer combinations were able to work universally across Insecta. Here, a set of three primer pairs were successful in amplifying COI of Mansonia annulata mosquito, a potential vector of Brugia malayi. By merging all three amplicons the whole COI was obtained. Primer pair TY-J-1460/C1N2087 amplified 5' region of COI, LepFl/LepRl the central and C1J2090/TL2N3014 the 3' region, generating COI amplicons of 650, 700 and 950 base pairs, respectively. When Ma. annulata sequences were compared with those from online sources, they formed a cluster group that is clearly distinct from other allied species.",

keywords = "COI, Mansonia annulata, Mitochondrial DNA, Primer",

author = "Chalalai Rueanghiran and Chamnarn Apiwathnasorn and Pradit Sangthong and Yudthana Samung and Jiraporn Ruangsittichai",

year = "2011",

language = "English",

volume = "42",

pages = "1381--1387",

journal = "Southeast Asian Journal of Tropical Medicine and Public Health",

issn = "0125-1562",

publisher = "SEAMEO TROPMED Network",

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Utility of a set of conserved mitochondrial cytochrome oxidase subunit I gene primers for Mansonia annulata identification. / Rueanghiran, Chalalai; Apiwathnasorn, Chamnarn; Sangthong, Pradit et al.
In: Southeast Asian Journal of Tropical Medicine and Public Health, Vol. 42, No. 6, 2011, p. 1381-1387.

Research output: Contribution to journal › Review article › peer-review

TY - JOUR

T1 - Utility of a set of conserved mitochondrial cytochrome oxidase subunit I gene primers for Mansonia annulata identification

AU - Rueanghiran, Chalalai

AU - Apiwathnasorn, Chamnarn

AU - Sangthong, Pradit

AU - Samung, Yudthana

AU - Ruangsittichai, Jiraporn

PY - 2011

Y1 - 2011

N2 - DNA-based identification system using the mitochondrial cytochrome oxidase subunit I (COI) gene has enabled validation of many species in certain taxonomic groups. These primer combinations were able to work universally across Insecta. Here, a set of three primer pairs were successful in amplifying COI of Mansonia annulata mosquito, a potential vector of Brugia malayi. By merging all three amplicons the whole COI was obtained. Primer pair TY-J-1460/C1N2087 amplified 5' region of COI, LepFl/LepRl the central and C1J2090/TL2N3014 the 3' region, generating COI amplicons of 650, 700 and 950 base pairs, respectively. When Ma. annulata sequences were compared with those from online sources, they formed a cluster group that is clearly distinct from other allied species.

AB - DNA-based identification system using the mitochondrial cytochrome oxidase subunit I (COI) gene has enabled validation of many species in certain taxonomic groups. These primer combinations were able to work universally across Insecta. Here, a set of three primer pairs were successful in amplifying COI of Mansonia annulata mosquito, a potential vector of Brugia malayi. By merging all three amplicons the whole COI was obtained. Primer pair TY-J-1460/C1N2087 amplified 5' region of COI, LepFl/LepRl the central and C1J2090/TL2N3014 the 3' region, generating COI amplicons of 650, 700 and 950 base pairs, respectively. When Ma. annulata sequences were compared with those from online sources, they formed a cluster group that is clearly distinct from other allied species.

KW - COI

KW - Mansonia annulata

KW - Mitochondrial DNA

KW - Primer

UR - https://www.scopus.com/pages/publications/84857668658

M3 - Review article

C2 - 22299406

AN - SCOPUS:84857668658

SN - 0125-1562

VL - 42

SP - 1381

EP - 1387

JO - Southeast Asian Journal of Tropical Medicine and Public Health

JF - Southeast Asian Journal of Tropical Medicine and Public Health

IS - 6

ER -

Utility of a set of conserved mitochondrial cytochrome oxidase subunit I gene primers for Mansonia annulata identification

Abstract

Keywords

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