Use of reverse transcription-polymerase chain reaction for cloning of coat protein-encoding genes of cymbidium mosaic virus

Pattana Srifah; Suvit Loprasert; Nanyawan Rungroj

doi:10.1016/S0378-1119(96)00419-2

Use of reverse transcription-polymerase chain reaction for cloning of coat protein-encoding genes of cymbidium mosaic virus

Pattana Srifah
, Suvit Loprasert
, Nanyawan Rungroj

Laboratory of Biotechnology
Kasetsart University

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

A reverse transcription-polymerase chain reaction (RT-PCR) has been developed for the cloning of coat protein-encoding genes (CP) of cymbidium mosaic virus (CyMV) isolated from three different infected species of Thai orchid: Cattleya, Mokara and Oncidium. The analysis of the compiled sequences of the cDNA clones shows a single open reading frame which encoded a CyMV CP gene. The gene is 669 nucleotides (nt) long and codes for a 23 761-Da protein. The nt sequences of CyMV CP from the Thai isolates showed that some of them differ at a single nt and share 97% homology, but all of them share only 88% homology to the Singapore Oncidium isolate. In addition, the CyMV CP, unlike that from other potexviruses, is distinctive and differs greatly from the amino acid composition deduced from the nt sequence of the CP.

Original language	English
Pages (from-to)	105-107
Number of pages	3
Journal	Gene
Volume	179
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1119(96)00419-2
Publication status	Published - 7 Nov 1996
Externally published	Yes

Keywords

Gene sequencing
Homology
Orchid
Plant virus
Potexvirus

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10.1016/S0378-1119(96)00419-2

Cite this

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title = "Use of reverse transcription-polymerase chain reaction for cloning of coat protein-encoding genes of cymbidium mosaic virus",

abstract = "A reverse transcription-polymerase chain reaction (RT-PCR) has been developed for the cloning of coat protein-encoding genes (CP) of cymbidium mosaic virus (CyMV) isolated from three different infected species of Thai orchid: Cattleya, Mokara and Oncidium. The analysis of the compiled sequences of the cDNA clones shows a single open reading frame which encoded a CyMV CP gene. The gene is 669 nucleotides (nt) long and codes for a 23 761-Da protein. The nt sequences of CyMV CP from the Thai isolates showed that some of them differ at a single nt and share 97\% homology, but all of them share only 88\% homology to the Singapore Oncidium isolate. In addition, the CyMV CP, unlike that from other potexviruses, is distinctive and differs greatly from the amino acid composition deduced from the nt sequence of the CP.",

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T1 - Use of reverse transcription-polymerase chain reaction for cloning of coat protein-encoding genes of cymbidium mosaic virus

AU - Srifah, Pattana

AU - Loprasert, Suvit

AU - Rungroj, Nanyawan

PY - 1996/11/7

Y1 - 1996/11/7

N2 - A reverse transcription-polymerase chain reaction (RT-PCR) has been developed for the cloning of coat protein-encoding genes (CP) of cymbidium mosaic virus (CyMV) isolated from three different infected species of Thai orchid: Cattleya, Mokara and Oncidium. The analysis of the compiled sequences of the cDNA clones shows a single open reading frame which encoded a CyMV CP gene. The gene is 669 nucleotides (nt) long and codes for a 23 761-Da protein. The nt sequences of CyMV CP from the Thai isolates showed that some of them differ at a single nt and share 97% homology, but all of them share only 88% homology to the Singapore Oncidium isolate. In addition, the CyMV CP, unlike that from other potexviruses, is distinctive and differs greatly from the amino acid composition deduced from the nt sequence of the CP.

AB - A reverse transcription-polymerase chain reaction (RT-PCR) has been developed for the cloning of coat protein-encoding genes (CP) of cymbidium mosaic virus (CyMV) isolated from three different infected species of Thai orchid: Cattleya, Mokara and Oncidium. The analysis of the compiled sequences of the cDNA clones shows a single open reading frame which encoded a CyMV CP gene. The gene is 669 nucleotides (nt) long and codes for a 23 761-Da protein. The nt sequences of CyMV CP from the Thai isolates showed that some of them differ at a single nt and share 97% homology, but all of them share only 88% homology to the Singapore Oncidium isolate. In addition, the CyMV CP, unlike that from other potexviruses, is distinctive and differs greatly from the amino acid composition deduced from the nt sequence of the CP.

KW - Gene sequencing

KW - Homology

KW - Orchid

KW - Plant virus

KW - Potexvirus

UR - https://www.scopus.com/pages/publications/0030574269

U2 - 10.1016/S0378-1119(96)00419-2

DO - 10.1016/S0378-1119(96)00419-2

M3 - Article

C2 - 8955635

AN - SCOPUS:0030574269

SN - 0378-1119

VL - 179

SP - 105

EP - 107

JO - Gene

JF - Gene

IS - 1

ER -

Use of reverse transcription-polymerase chain reaction for cloning of coat protein-encoding genes of cymbidium mosaic virus

Abstract

Keywords

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