Three non‐repeated transmission blocking epitopes recognized in the 21 kD surface antigen of zygotes‐ookinetes of Plasmodium berghei

NEDNAPIS TIRAWANCHAI; ROBERT E. SINDEN

doi:10.1111/j.1365-3024.1990.tb00979.x

Three non‐repeated transmission blocking epitopes recognized in the 21 kD surface antigen of zygotes‐ookinetes of Plasmodium berghei

NEDNAPIS TIRAWANCHAI
, ROBERT E. SINDEN

Imperial College London

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Summary Two‐site and competitive ELISA have been developed against a surface antigen of zygotes‐ookinetes of Plasmodium berghei using monoclonal antibodies (MoAbs) which block transmission of the parasite to the mosquito. Three such MoAbs have been studied, each of which recognized a protein of an M_r 21 kD (Pbs21) using immunoblot techniques. The assays showed that there are at least 3 single B‐cell epitopes expressed in Pbs21. One epitope recognized by MoAb 17.9 is conformation dependent and antibodies bound to it interfered with other MoAbs (12.1 and 13.1) each recognizing a different, apparently linear epitope. Glycosylation might not be relevant to the binding of any of the antibodies tested to their respective epitopes.

Original language	English
Pages (from-to)	435-446
Number of pages	12
Journal	Parasite Immunology
Volume	12
Issue number	4-5
DOIs	https://doi.org/10.1111/j.1365-3024.1990.tb00979.x
Publication status	Published - Sept 1990
Externally published	Yes

Keywords

Plasmodium berghei
epitope
transmission‐blocking immunity

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10.1111/j.1365-3024.1990.tb00979.x

Cite this

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title = "Three non‐repeated transmission blocking epitopes recognized in the 21 kD surface antigen of zygotes‐ookinetes of Plasmodium berghei",

abstract = "Summary Two‐site and competitive ELISA have been developed against a surface antigen of zygotes‐ookinetes of Plasmodium berghei using monoclonal antibodies (MoAbs) which block transmission of the parasite to the mosquito. Three such MoAbs have been studied, each of which recognized a protein of an Mr 21 kD (Pbs21) using immunoblot techniques. The assays showed that there are at least 3 single B‐cell epitopes expressed in Pbs21. One epitope recognized by MoAb 17.9 is conformation dependent and antibodies bound to it interfered with other MoAbs (12.1 and 13.1) each recognizing a different, apparently linear epitope. Glycosylation might not be relevant to the binding of any of the antibodies tested to their respective epitopes.",

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AU - TIRAWANCHAI, NEDNAPIS

AU - SINDEN, ROBERT E.

PY - 1990/9

Y1 - 1990/9

N2 - Summary Two‐site and competitive ELISA have been developed against a surface antigen of zygotes‐ookinetes of Plasmodium berghei using monoclonal antibodies (MoAbs) which block transmission of the parasite to the mosquito. Three such MoAbs have been studied, each of which recognized a protein of an Mr 21 kD (Pbs21) using immunoblot techniques. The assays showed that there are at least 3 single B‐cell epitopes expressed in Pbs21. One epitope recognized by MoAb 17.9 is conformation dependent and antibodies bound to it interfered with other MoAbs (12.1 and 13.1) each recognizing a different, apparently linear epitope. Glycosylation might not be relevant to the binding of any of the antibodies tested to their respective epitopes.

AB - Summary Two‐site and competitive ELISA have been developed against a surface antigen of zygotes‐ookinetes of Plasmodium berghei using monoclonal antibodies (MoAbs) which block transmission of the parasite to the mosquito. Three such MoAbs have been studied, each of which recognized a protein of an Mr 21 kD (Pbs21) using immunoblot techniques. The assays showed that there are at least 3 single B‐cell epitopes expressed in Pbs21. One epitope recognized by MoAb 17.9 is conformation dependent and antibodies bound to it interfered with other MoAbs (12.1 and 13.1) each recognizing a different, apparently linear epitope. Glycosylation might not be relevant to the binding of any of the antibodies tested to their respective epitopes.

KW - Plasmodium berghei

KW - epitope

KW - transmission‐blocking immunity

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AN - SCOPUS:0025362061

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JF - Parasite Immunology

IS - 4-5

ER -

Three non‐repeated transmission blocking epitopes recognized in the 21 kD surface antigen of zygotes‐ookinetes of Plasmodium berghei

Abstract

Keywords

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