The use of co-immobilization of Trichosporon cutaneum and Bacillus licheniformis for a BOD sensor

L. Suriyawattanakul; W. Surareungchai; P. Sritongkam; M. Tanticharoen; K. Kirtikara

doi:10.1007/s00253-002-0980-1

The use of co-immobilization of Trichosporon cutaneum and Bacillus licheniformis for a BOD sensor

L. Suriyawattanakul
, W. Surareungchai
, P. Sritongkam
, M. Tanticharoen
, K. Kirtikara

K. Mongkut's Univ. Technol. Thonburi
National Science and Technology Development Agency (NSTDA)

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The microorganisms Trichosporon cutaneum and Bacillus licheniformis were used to develop a microbial biochemical oxygen demand (BOD) sensor. It was found that T. cutaneum gave a greater response to glucose, whereas B. licheniformis gave a better response to glutamic acid. Hence, co-immobilized T. cutaneum and B. licheniformis were used to construct a glucose and glutamic acid sensor with improved sensitivity and dynamic range. A membrane loading of T. cutaneum at 1.1×10⁸ cells ml^-1 cm^-2 and B. licheniformis at 2.2×10⁸ cells ml^-1 cm^-2 gave the optimum result: a linear range up to 40 mg BOD l^-1 with a sensitivity of 5.84 nA mg^-1 BOD l. The optimized BOD sensor showed operation stability for 58 intermittent batch measurements, with a standard deviation of 0.0362 and a variance of 0.131 nA. The response time of the co-immobilized microbial BOD sensor was within 5-10 min by steady-state measurement and the detection limit was 0.5 mg BOD l^-1. The BOD sensor was insensitive to pH in the range of pH 6.8-7.2.

Original language	English
Pages (from-to)	40-44
Number of pages	5
Journal	Applied Microbiology and Biotechnology
Volume	59
Issue number	1
DOIs	https://doi.org/10.1007/s00253-002-0980-1
Publication status	Published - 2002
Externally published	Yes

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title = "The use of co-immobilization of Trichosporon cutaneum and Bacillus licheniformis for a BOD sensor",

abstract = "The microorganisms Trichosporon cutaneum and Bacillus licheniformis were used to develop a microbial biochemical oxygen demand (BOD) sensor. It was found that T. cutaneum gave a greater response to glucose, whereas B. licheniformis gave a better response to glutamic acid. Hence, co-immobilized T. cutaneum and B. licheniformis were used to construct a glucose and glutamic acid sensor with improved sensitivity and dynamic range. A membrane loading of T. cutaneum at 1.1{\texttimes}108 cells ml-1 cm-2 and B. licheniformis at 2.2{\texttimes}108 cells ml-1 cm-2 gave the optimum result: a linear range up to 40 mg BOD l-1 with a sensitivity of 5.84 nA mg-1 BOD l. The optimized BOD sensor showed operation stability for 58 intermittent batch measurements, with a standard deviation of 0.0362 and a variance of 0.131 nA. The response time of the co-immobilized microbial BOD sensor was within 5-10 min by steady-state measurement and the detection limit was 0.5 mg BOD l-1. The BOD sensor was insensitive to pH in the range of pH 6.8-7.2.",

author = "L. Suriyawattanakul and W. Surareungchai and P. Sritongkam and M. Tanticharoen and K. Kirtikara",

year = "2002",

doi = "10.1007/s00253-002-0980-1",

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T1 - The use of co-immobilization of Trichosporon cutaneum and Bacillus licheniformis for a BOD sensor

AU - Suriyawattanakul, L.

AU - Surareungchai, W.

AU - Sritongkam, P.

AU - Tanticharoen, M.

AU - Kirtikara, K.

PY - 2002

Y1 - 2002

N2 - The microorganisms Trichosporon cutaneum and Bacillus licheniformis were used to develop a microbial biochemical oxygen demand (BOD) sensor. It was found that T. cutaneum gave a greater response to glucose, whereas B. licheniformis gave a better response to glutamic acid. Hence, co-immobilized T. cutaneum and B. licheniformis were used to construct a glucose and glutamic acid sensor with improved sensitivity and dynamic range. A membrane loading of T. cutaneum at 1.1×108 cells ml-1 cm-2 and B. licheniformis at 2.2×108 cells ml-1 cm-2 gave the optimum result: a linear range up to 40 mg BOD l-1 with a sensitivity of 5.84 nA mg-1 BOD l. The optimized BOD sensor showed operation stability for 58 intermittent batch measurements, with a standard deviation of 0.0362 and a variance of 0.131 nA. The response time of the co-immobilized microbial BOD sensor was within 5-10 min by steady-state measurement and the detection limit was 0.5 mg BOD l-1. The BOD sensor was insensitive to pH in the range of pH 6.8-7.2.

AB - The microorganisms Trichosporon cutaneum and Bacillus licheniformis were used to develop a microbial biochemical oxygen demand (BOD) sensor. It was found that T. cutaneum gave a greater response to glucose, whereas B. licheniformis gave a better response to glutamic acid. Hence, co-immobilized T. cutaneum and B. licheniformis were used to construct a glucose and glutamic acid sensor with improved sensitivity and dynamic range. A membrane loading of T. cutaneum at 1.1×108 cells ml-1 cm-2 and B. licheniformis at 2.2×108 cells ml-1 cm-2 gave the optimum result: a linear range up to 40 mg BOD l-1 with a sensitivity of 5.84 nA mg-1 BOD l. The optimized BOD sensor showed operation stability for 58 intermittent batch measurements, with a standard deviation of 0.0362 and a variance of 0.131 nA. The response time of the co-immobilized microbial BOD sensor was within 5-10 min by steady-state measurement and the detection limit was 0.5 mg BOD l-1. The BOD sensor was insensitive to pH in the range of pH 6.8-7.2.

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AN - SCOPUS:0036325224

SN - 0175-7598

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JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 1

ER -

The use of co-immobilization of Trichosporon cutaneum and Bacillus licheniformis for a BOD sensor

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