The influence of pre- and post-ovulatory insemination on sperm distribution in the oviduct, accessory sperm to the zona pellucida, fertilisation rate and embryo development in sows

The aim of present study was to investigate the influence of pre-compared with post-ovulatory insemination, on the distribution of spermatozoa in the oviduct, the accessory sperm counts on the zona pellucida and early embryonic development. Thirty-six crossbred multiparous sows (Swedish Landrace x Swedish Yorkshire) were artificially inseminated once either at 20-15 h before (group AIB) or at 15-20 h after (group AIA) ovulation by using a pooled semen of two boars. Thereafter, they were randomly allocated to one of five groups: slaughter at 5-6 h after AI (group I-AIB), at 20-25 h after ovulation (groups II-AIB and II-AIA), at 70 h after ovulation (groups III-AIB and III-AIA), on day 11 (groups IV-AIB and IV-AIA, first day of standing oestrus = day 1) and on day 19 (groups V-AIB and V-AIA). The plasma levels of oestradiol-17β and progesterone differed significantly (P ≤ 0.05 and P ≤ 0.001, respectively) between AI before (group AIB) and after (group AIA) ovulation. All sows inseminated before ovulation (group I-AIB) had spermatozoa in the UTJ but this was found only in one of the late inseminated sows (group II-AIA). The number of oocytes with spermatozoa in the ZP differed significantly (P ≤ 0.001) between 'group II-AIB' and 'group II-AIA'. Comparing fertilisation rate, the fertilisation rate in 'group III-AIB' was significantly (P ≤ 0.001) higher than in 'group III-AIA'. A larger number of recovered embryos (on days 11 and 19) per sow in groups IV-AIB and V-AIB compared with IV-AIA and V-AIA were found. The embryos in group IV-AIB were also larger than embryos in group IV-AIA. In group V-AIA, no embryos were found. In conclusion, the results of this study showed that if insemination was performed at 15-20 after ovulation in sows that still were in standing oestrus, the transport of spermatozoa to the UTJ and oviduct was impaired and a lower proportion of oocytes with accessory spermatozoa to the zona pellucida was found compared with insemination before ovulation. In the late inseminated sows, fertilised oocytes and developed embryos were observed up to day 11 but no embryos were found at day 19.