TaqMan qPCR for detection and quantification of mitochondrial DNA from toxic pufferfish species

Tassanee Luekasemsuk; Nathinee Panvisavas; Soraya Chaturongakul

doi:10.1016/j.toxicon.2015.05.016

TaqMan qPCR for detection and quantification of mitochondrial DNA from toxic pufferfish species

Tassanee Luekasemsuk
, Nathinee Panvisavas
, Soraya Chaturongakul

Mahidol University

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Outbreaks of pufferfish food poisoning have been reported worldwide, most were from unsuitable food preparation and adulteration. In order to rapidly detect pufferfish adulterant in processed foods, we developed and proposed the use of a TaqMan probe-based quantitative PCR. The designed detection oligos targeted a unique region in 16S rDNA of toxic marine pufferfish in Tetraodontidae Family and gave a positive signal at ≥1.75 pg of genomic DNA. Non-target DNA samples from other fish, chicken, and beef were negative.

Original language	English
Pages (from-to)	43-47
Number of pages	5
Journal	Toxicon
Volume	102
DOIs	https://doi.org/10.1016/j.toxicon.2015.05.016
Publication status	Published - 11 Jun 2015
Externally published	Yes

Keywords

Pufferfish
Quantitative real-time detection
TaqMan PCR
Tetraodontidae

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10.1016/j.toxicon.2015.05.016

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abstract = "Outbreaks of pufferfish food poisoning have been reported worldwide, most were from unsuitable food preparation and adulteration. In order to rapidly detect pufferfish adulterant in processed foods, we developed and proposed the use of a TaqMan probe-based quantitative PCR. The designed detection oligos targeted a unique region in 16S rDNA of toxic marine pufferfish in Tetraodontidae Family and gave a positive signal at ≥1.75 pg of genomic DNA. Non-target DNA samples from other fish, chicken, and beef were negative.",

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AU - Luekasemsuk, Tassanee

AU - Panvisavas, Nathinee

AU - Chaturongakul, Soraya

PY - 2015/6/11

Y1 - 2015/6/11

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KW - Pufferfish

KW - Quantitative real-time detection

KW - TaqMan PCR

KW - Tetraodontidae

UR - https://www.scopus.com/pages/publications/84930959719

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DO - 10.1016/j.toxicon.2015.05.016

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VL - 102

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EP - 47

JO - Toxicon

JF - Toxicon

ER -

TaqMan qPCR for detection and quantification of mitochondrial DNA from toxic pufferfish species

Abstract

Keywords

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