Survival of Burkholderia pseudomallei in distilled water for 16 years

Apinya Pumpuang; Narisara Chantratita; Chanthiwa Wikraiphat; Natnaree Saiprom; Nicholas P.J. Day; Sharon J. Peacock; Vanaporn Wuthiekanun

doi:10.1016/j.trstmh.2011.06.004

Survival of Burkholderia pseudomallei in distilled water for 16 years

Apinya Pumpuang
, Narisara Chantratita
, Chanthiwa Wikraiphat
, Natnaree Saiprom
, Nicholas P.J. Day
, Sharon J. Peacock
, Vanaporn Wuthiekanun

Department of Microbiology and Immunology

Faculty of Tropical Medicine, Mahidol University
Mahidol University

Research output: Contribution to journal › Article › peer-review

77 Citations (Scopus)

Abstract

Burkholderia pseudomallei was examined after being maintained in distilled water at 25°C for 16 years. The Gram stain was atypical (pale pink cocci or coccobacilli). The estimated number of live and dead B. pseudomallei was 3.8×10 ⁷ cells/ml and 1.4×10 ⁵ cells/ml, respectively. A colony count on agar of 1.0×10 ⁶ cfu/ml suggested that a proportion of cells were in a viable but non-culturable state. Colony morphology was different from the parental isolate for 84% of colonies. Pulsed-field gel electrophoresis analysis of AvrII DNA restriction fragments revealed six different but related banding patterns, which may represent genomic rearrangement.

Original language	English
Pages (from-to)	598-600
Number of pages	3
Journal	Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume	105
Issue number	10
DOIs	https://doi.org/10.1016/j.trstmh.2011.06.004
Publication status	Published - Oct 2011

Keywords

Burkholderia pseudomallei
Colony morphology
Genetic alteration
Melioidosis
PFGE
Survival in water

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10.1016/j.trstmh.2011.06.004

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title = "Survival of Burkholderia pseudomallei in distilled water for 16 years",

abstract = "Burkholderia pseudomallei was examined after being maintained in distilled water at 25°C for 16 years. The Gram stain was atypical (pale pink cocci or coccobacilli). The estimated number of live and dead B. pseudomallei was 3.8{\texttimes}10 7 cells/ml and 1.4{\texttimes}10 5 cells/ml, respectively. A colony count on agar of 1.0{\texttimes}10 6 cfu/ml suggested that a proportion of cells were in a viable but non-culturable state. Colony morphology was different from the parental isolate for 84\% of colonies. Pulsed-field gel electrophoresis analysis of AvrII DNA restriction fragments revealed six different but related banding patterns, which may represent genomic rearrangement.",

keywords = "Burkholderia pseudomallei, Colony morphology, Genetic alteration, Melioidosis, PFGE, Survival in water",

author = "Apinya Pumpuang and Narisara Chantratita and Chanthiwa Wikraiphat and Natnaree Saiprom and Day, \{Nicholas P.J.\} and Peacock, \{Sharon J.\} and Vanaporn Wuthiekanun",

year = "2011",

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doi = "10.1016/j.trstmh.2011.06.004",

language = "English",

volume = "105",

pages = "598--600",

journal = "Transactions of the Royal Society of Tropical Medicine and Hygiene",

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AU - Pumpuang, Apinya

AU - Chantratita, Narisara

AU - Wikraiphat, Chanthiwa

AU - Saiprom, Natnaree

AU - Day, Nicholas P.J.

AU - Peacock, Sharon J.

AU - Wuthiekanun, Vanaporn

PY - 2011/10

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AB - Burkholderia pseudomallei was examined after being maintained in distilled water at 25°C for 16 years. The Gram stain was atypical (pale pink cocci or coccobacilli). The estimated number of live and dead B. pseudomallei was 3.8×10 7 cells/ml and 1.4×10 5 cells/ml, respectively. A colony count on agar of 1.0×10 6 cfu/ml suggested that a proportion of cells were in a viable but non-culturable state. Colony morphology was different from the parental isolate for 84% of colonies. Pulsed-field gel electrophoresis analysis of AvrII DNA restriction fragments revealed six different but related banding patterns, which may represent genomic rearrangement.

KW - Burkholderia pseudomallei

KW - Colony morphology

KW - Genetic alteration

KW - Melioidosis

KW - PFGE

KW - Survival in water

UR - https://www.scopus.com/pages/publications/80052967899

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DO - 10.1016/j.trstmh.2011.06.004

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SN - 0035-9203

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JO - Transactions of the Royal Society of Tropical Medicine and Hygiene

JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

IS - 10

ER -

Survival of Burkholderia pseudomallei in distilled water for 16 years

Abstract

Keywords

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