Spatially selecting a single cell for lysis using light-induced electric fields

Christian Witte; Clemens Kremer; Mayuree Chanasakulniyom; Julien Reboud; Rab Wilson; Jonathan M. Cooper; Steven L. Neale

doi:10.1002/smll.201400247

Spatially selecting a single cell for lysis using light-induced electric fields

Christian Witte
, Clemens Kremer
, Mayuree Chanasakulniyom
, Julien Reboud
, Rab Wilson
, Jonathan M. Cooper
, Steven L. Neale

University of Glasgow

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

An optoelectronic tweezing (OET) device, within an integrated microfluidic channel, is used to precisely select single cells for lysis among dense populations. Cells to be lysed are exposed to higher electrical fields than their neighbours by illuminating a photoconductive film underneath them. Using beam spot sizes as low as 2.5 μm, 100% lysis efficiency is reached in <1 min allowing the targeted lysis of cells.

Original language	English
Pages (from-to)	3026-3031
Number of pages	6
Journal	Small
Volume	10
Issue number	15
DOIs	https://doi.org/10.1002/smll.201400247
Publication status	Published - 13 Aug 2014
Externally published	Yes

Keywords

electroporation
lysis
optoelectronic
single cell

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10.1002/smll.201400247

Cite this

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abstract = "An optoelectronic tweezing (OET) device, within an integrated microfluidic channel, is used to precisely select single cells for lysis among dense populations. Cells to be lysed are exposed to higher electrical fields than their neighbours by illuminating a photoconductive film underneath them. Using beam spot sizes as low as 2.5 μm, 100\% lysis efficiency is reached in <1 min allowing the targeted lysis of cells.",

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AU - Witte, Christian

AU - Kremer, Clemens

AU - Chanasakulniyom, Mayuree

AU - Reboud, Julien

AU - Wilson, Rab

AU - Cooper, Jonathan M.

AU - Neale, Steven L.

PY - 2014/8/13

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AB - An optoelectronic tweezing (OET) device, within an integrated microfluidic channel, is used to precisely select single cells for lysis among dense populations. Cells to be lysed are exposed to higher electrical fields than their neighbours by illuminating a photoconductive film underneath them. Using beam spot sizes as low as 2.5 μm, 100% lysis efficiency is reached in <1 min allowing the targeted lysis of cells.

KW - electroporation

KW - lysis

KW - optoelectronic

KW - single cell

UR - https://www.scopus.com/pages/publications/84905657527

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DO - 10.1002/smll.201400247

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Spatially selecting a single cell for lysis using light-induced electric fields

Abstract

Keywords

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