TY - GEN
T1 - Singlet oxygen formation in photodynamic therapy using guaiazulene and red laser
T2 - 17th International Photodynamic Association World Congress 2019
AU - Mangkhalathon, Ampika
AU - Teerakapong, Aroon
AU - Morales, Noppawan Phumala
AU - Morkmued, Supawich
AU - Damrongrungruang, Teerasak
N1 - Publisher Copyright:
© 2019 SPIE.
PY - 2019
Y1 - 2019
N2 - Guaiazulene is one of the natural azulene derivatives, which has anti-inflammatory as well as photosensitizer property and can be activated by highly penetrating red light. The proper singlet oxygen from this activation will lead to a useful strategy for specific treating unwanted cells. Objective: To measure in vitro amount of singlet oxygen produced from PDT reaction using various guaiazulene concentrations and red laser energies. Methods: Guaiazulene were prepared by diluting in methanol and mixed with 2,2,5,5-tetramethyl-3-pyrroline-3-carboxamide (TPC) to obtain 1, 2, 5, 15, 25, 35, 100, and 500 μM. Erythrosine (10 μM in each light energies) were used as positive control. Then, irradiations were performed with various red laser energies (625 nm, 151.5 mW/cm2, distance from laser tip to bottom of the well = 8 mm, and continuous mode) at 0, 4, and 8 J/cm2 in a black 96-well plate. The amount of singlet oxygen was measured in triplicate by electron spin resonance spectrometer (Bruker, Germany) at 0, 3, and 5 minutes after irradiation. Kruskal-Wallis and Mann-Whitney U test were used for statistical analyses with significant differences at p-value < 0.05. Results: The average singlet oxygen of 5 μM group significantly different from no guiazulene group (p < 0.01). Regarding the energy, both 4 and 8 J/cm2 resulted in relatively equal amounts of singlet oxygen. Conclusion: Five μM of guaiazulene activated by 625 nm laser at energy densities 4-8 J/cm2 dramatically generated singlet oxygen in current in vitro setting.
AB - Guaiazulene is one of the natural azulene derivatives, which has anti-inflammatory as well as photosensitizer property and can be activated by highly penetrating red light. The proper singlet oxygen from this activation will lead to a useful strategy for specific treating unwanted cells. Objective: To measure in vitro amount of singlet oxygen produced from PDT reaction using various guaiazulene concentrations and red laser energies. Methods: Guaiazulene were prepared by diluting in methanol and mixed with 2,2,5,5-tetramethyl-3-pyrroline-3-carboxamide (TPC) to obtain 1, 2, 5, 15, 25, 35, 100, and 500 μM. Erythrosine (10 μM in each light energies) were used as positive control. Then, irradiations were performed with various red laser energies (625 nm, 151.5 mW/cm2, distance from laser tip to bottom of the well = 8 mm, and continuous mode) at 0, 4, and 8 J/cm2 in a black 96-well plate. The amount of singlet oxygen was measured in triplicate by electron spin resonance spectrometer (Bruker, Germany) at 0, 3, and 5 minutes after irradiation. Kruskal-Wallis and Mann-Whitney U test were used for statistical analyses with significant differences at p-value < 0.05. Results: The average singlet oxygen of 5 μM group significantly different from no guiazulene group (p < 0.01). Regarding the energy, both 4 and 8 J/cm2 resulted in relatively equal amounts of singlet oxygen. Conclusion: Five μM of guaiazulene activated by 625 nm laser at energy densities 4-8 J/cm2 dramatically generated singlet oxygen in current in vitro setting.
KW - Electron spin resonance spectrometer
KW - Guaiazulene
KW - Photodynamic therapy
KW - Red laser
KW - Singlet oxygen
KW - TPC
UR - https://www.scopus.com/pages/publications/85075896563
U2 - 10.1117/12.2525414
DO - 10.1117/12.2525414
M3 - Conference contribution
AN - SCOPUS:85075896563
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - 17th International Photodynamic Association World Congress
A2 - Hasan, Tayyaba
PB - SPIE
Y2 - 28 June 2019 through 4 July 2019
ER -