Short report: Evaluation of loop-mediated isothermal amplification (LAMP) for malaria diagnosis in a field setting

Jeeraphat Sirichaisinthop; Sureemas Buates; Risa Watanabe; Eun Taek Han; Wachira Suktawonjaroenpon; Somporn Krasaesub; Satoru Takeo; Takafumi Tsuboi; Jetsumon Sattabongkot

doi:10.4269/ajtmh.2011.10-0676

Short report: Evaluation of loop-mediated isothermal amplification (LAMP) for malaria diagnosis in a field setting

Jeeraphat Sirichaisinthop
, Sureemas Buates
, Risa Watanabe
, Eun Taek Han
, Wachira Suktawonjaroenpon
, Somporn Krasaesub
, Satoru Takeo
, Takafumi Tsuboi
, Jetsumon Sattabongkot

Malaria Research Center

Vector Borne Disease Training Center
Mahidol University
Ehime University
School of Medicine, Kangwon National University
US Army Medical Directorate of the Armed Forces Research Institute of Medical Sciences

Research output: Contribution to journal › Article › peer-review

71 Citations (Scopus)

Abstract

We used the loop-mediated isothermal amplification (LAMP) method developed by our group for malaria diagnosis with genus-specific and species-specific primers for the four human malaria parasites at a field clinic in comparison with standard microscopy. Among 110 blood samples collected from the malaria clinic in Thailand, LAMP detected 59 of 60 samples positive by microscopy (sensitivity = 98.3%) and none of the 50 microscopy-negative samples (specificity = 100%). Negative predictive value (NPV) and positive predictive value (PPV) of LAMP were 98% and 100%, respectively. These results indicate that LAMP is an effective tool for malaria diagnosis at a field clinic in a field setting.

Original language	English
Pages (from-to)	594-596
Number of pages	3
Journal	American Journal of Tropical Medicine and Hygiene
Volume	85
Issue number	4
DOIs	https://doi.org/10.4269/ajtmh.2011.10-0676
Publication status	Published - Oct 2011

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SDG 3 Good Health and Well-being

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10.4269/ajtmh.2011.10-0676

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Sirichaisinthop, J., Buates, S., Watanabe, R., Han, E. T., Suktawonjaroenpon, W., Krasaesub, S., Takeo, S., Tsuboi, T., & Sattabongkot, J. (2011). Short report: Evaluation of loop-mediated isothermal amplification (LAMP) for malaria diagnosis in a field setting. American Journal of Tropical Medicine and Hygiene, 85(4), 594-596. https://doi.org/10.4269/ajtmh.2011.10-0676

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abstract = "We used the loop-mediated isothermal amplification (LAMP) method developed by our group for malaria diagnosis with genus-specific and species-specific primers for the four human malaria parasites at a field clinic in comparison with standard microscopy. Among 110 blood samples collected from the malaria clinic in Thailand, LAMP detected 59 of 60 samples positive by microscopy (sensitivity = 98.3\%) and none of the 50 microscopy-negative samples (specificity = 100\%). Negative predictive value (NPV) and positive predictive value (PPV) of LAMP were 98\% and 100\%, respectively. These results indicate that LAMP is an effective tool for malaria diagnosis at a field clinic in a field setting.",

author = "Jeeraphat Sirichaisinthop and Sureemas Buates and Risa Watanabe and Han, \{Eun Taek\} and Wachira Suktawonjaroenpon and Somporn Krasaesub and Satoru Takeo and Takafumi Tsuboi and Jetsumon Sattabongkot",

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Sirichaisinthop, J, Buates, S, Watanabe, R, Han, ET, Suktawonjaroenpon, W, Krasaesub, S, Takeo, S, Tsuboi, T & Sattabongkot, J 2011, 'Short report: Evaluation of loop-mediated isothermal amplification (LAMP) for malaria diagnosis in a field setting', American Journal of Tropical Medicine and Hygiene, vol. 85, no. 4, pp. 594-596. https://doi.org/10.4269/ajtmh.2011.10-0676

TY - JOUR

T1 - Short report

T2 - Evaluation of loop-mediated isothermal amplification (LAMP) for malaria diagnosis in a field setting

AU - Sirichaisinthop, Jeeraphat

AU - Buates, Sureemas

AU - Watanabe, Risa

AU - Han, Eun Taek

AU - Suktawonjaroenpon, Wachira

AU - Krasaesub, Somporn

AU - Takeo, Satoru

AU - Tsuboi, Takafumi

AU - Sattabongkot, Jetsumon

PY - 2011/10

Y1 - 2011/10

N2 - We used the loop-mediated isothermal amplification (LAMP) method developed by our group for malaria diagnosis with genus-specific and species-specific primers for the four human malaria parasites at a field clinic in comparison with standard microscopy. Among 110 blood samples collected from the malaria clinic in Thailand, LAMP detected 59 of 60 samples positive by microscopy (sensitivity = 98.3%) and none of the 50 microscopy-negative samples (specificity = 100%). Negative predictive value (NPV) and positive predictive value (PPV) of LAMP were 98% and 100%, respectively. These results indicate that LAMP is an effective tool for malaria diagnosis at a field clinic in a field setting.

AB - We used the loop-mediated isothermal amplification (LAMP) method developed by our group for malaria diagnosis with genus-specific and species-specific primers for the four human malaria parasites at a field clinic in comparison with standard microscopy. Among 110 blood samples collected from the malaria clinic in Thailand, LAMP detected 59 of 60 samples positive by microscopy (sensitivity = 98.3%) and none of the 50 microscopy-negative samples (specificity = 100%). Negative predictive value (NPV) and positive predictive value (PPV) of LAMP were 98% and 100%, respectively. These results indicate that LAMP is an effective tool for malaria diagnosis at a field clinic in a field setting.

UR - https://www.scopus.com/pages/publications/80053913069

U2 - 10.4269/ajtmh.2011.10-0676

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AN - SCOPUS:80053913069

SN - 0002-9637

VL - 85

SP - 594

EP - 596

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

IS - 4

ER -

Short report: Evaluation of loop-mediated isothermal amplification (LAMP) for malaria diagnosis in a field setting

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