TY - GEN
T1 - Saccharification of delignified sugarcane bagasse for lactic acid fermentation using Lactobacillus sp
AU - Jonglertjunya, Woranart
AU - Srimarut, Nattawee
AU - Lukkanakul, Nawapol
AU - Sakdaronnarong, Chularat
AU - Nasongkla, Norased
PY - 2014
Y1 - 2014
N2 - Delignified sugarcane bagasse (SCB) by solvent extraction was carried out at moderate condition (90°C, 4 h) in the presence of acid catalyst. To investigate the dissolution of lignin into solvent, different solvents were utilized during lignin extraction process. Delignified SCB was further hydrolyzed by cell wall degrading enzyme complexes prior to sugar determination. The results showed that n-butanol was the most promising solvent enhancing lignin dissolution which in turn led to highest yield of glucose (63.1% based on treated SCB) and no xylose was detected in hydrolysate. The lignin extraction by n-butanol in the presence of H2SO4 and subsequent saccharification process were then scaled up for lactic acid production. The maximum lactic acid was obtained at 25.7 ± 0.2 g/l from L. casei fermentation after 96 hours when sugarcane bagasse hydrolysate containing 25.6±1.4 g/l initial glucose concentration was used as substrate.
AB - Delignified sugarcane bagasse (SCB) by solvent extraction was carried out at moderate condition (90°C, 4 h) in the presence of acid catalyst. To investigate the dissolution of lignin into solvent, different solvents were utilized during lignin extraction process. Delignified SCB was further hydrolyzed by cell wall degrading enzyme complexes prior to sugar determination. The results showed that n-butanol was the most promising solvent enhancing lignin dissolution which in turn led to highest yield of glucose (63.1% based on treated SCB) and no xylose was detected in hydrolysate. The lignin extraction by n-butanol in the presence of H2SO4 and subsequent saccharification process were then scaled up for lactic acid production. The maximum lactic acid was obtained at 25.7 ± 0.2 g/l from L. casei fermentation after 96 hours when sugarcane bagasse hydrolysate containing 25.6±1.4 g/l initial glucose concentration was used as substrate.
KW - Lactic acid fermentation
KW - Lactobacillus sp
KW - Saccharification and fermentation
KW - Sugarcane bagasse
UR - https://www.scopus.com/pages/publications/84901494870
U2 - 10.4028/www.scientific.net/AMR.931-932.1597
DO - 10.4028/www.scientific.net/AMR.931-932.1597
M3 - Conference contribution
AN - SCOPUS:84901494870
SN - 9783038350903
T3 - Advanced Materials Research
SP - 1597
EP - 1601
BT - KKU International Engineering Conference
PB - Trans Tech Publications
T2 - 5th KKU International Engineering Conference 2014, KKU-IENC 2014
Y2 - 27 March 2014 through 29 March 2014
ER -