Resistance to the "last resort" antibiotic colistin: A single-zinc mechanism for phosphointermediate formation in MCR enzymes

Emily Lythell; Emily Lythell; Reynier Suardíaz; Reynier Suardíaz; Reynier Suardíaz; Reynier Suardíaz; Philip Hinchliffe; Chonnikan Hanpaibool; Surawit Visitsatthawong; A. Sofia F. Oliveira; Eric J.M. Lang; Panida Surawatanawong; Vannajan Sanghiran Lee; Thanyada Rungrotmongkol; Thanyada Rungrotmongkol; Natalie Fey; James Spencer; Adrian J. Mulholland

doi:10.1039/d0cc02520h

Resistance to the "last resort" antibiotic colistin: A single-zinc mechanism for phosphointermediate formation in MCR enzymes

Emily Lythell
, Emily Lythell
, Reynier Suardíaz
, Reynier Suardíaz
, Reynier Suardíaz
, Reynier Suardíaz
, Philip Hinchliffe
, Chonnikan Hanpaibool
, Surawit Visitsatthawong
, A. Sofia F. Oliveira
, Eric J.M. Lang
, Panida Surawatanawong
, Vannajan Sanghiran Lee
, Thanyada Rungrotmongkol
, Thanyada Rungrotmongkol
, Natalie Fey
, James Spencer
, Adrian J. Mulholland

University of Bristol
University of Portsmouth
Universidad Complutense de Madrid
Chulalongkorn University
Mahidol University
University of Malaya

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

MCR (mobile colistin resistance) enzymes catalyse phosphoethanolamine (PEA) addition to bacterial lipid A, threatening the "last-resort"antibiotic colistin. Molecular dynamics and density functional theory simulations indicate that monozinc MCR supports PEA transfer to the Thr285 acceptor, positioning MCR as a mono-rather than multinuclear member of the alkaline phosphatase superfamily.

Original language	English
Pages (from-to)	6874-6877
Number of pages	4
Journal	Chemical Communications
Volume	56
Issue number	50
DOIs	https://doi.org/10.1039/d0cc02520h
Publication status	Published - 25 Jun 2020
Externally published	Yes

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10.1039/d0cc02520h

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Lythell, E., Lythell, E., Suardíaz, R., Suardíaz, R., Suardíaz, R., Suardíaz, R., Hinchliffe, P., Hanpaibool, C., Visitsatthawong, S., Oliveira, A. S. F., Lang, E. J. M., Surawatanawong, P., Lee, V. S., Rungrotmongkol, T., Rungrotmongkol, T., Fey, N., Spencer, J., & Mulholland, A. J. (2020). Resistance to the "last resort" antibiotic colistin: A single-zinc mechanism for phosphointermediate formation in MCR enzymes. Chemical Communications, 56(50), 6874-6877. https://doi.org/10.1039/d0cc02520h

@article{b11f440c00cb4371a341fe3d95d862cb,

title = "Resistance to the {"}last resort{"} antibiotic colistin: A single-zinc mechanism for phosphointermediate formation in MCR enzymes",

abstract = "MCR (mobile colistin resistance) enzymes catalyse phosphoethanolamine (PEA) addition to bacterial lipid A, threatening the {"}last-resort{"}antibiotic colistin. Molecular dynamics and density functional theory simulations indicate that monozinc MCR supports PEA transfer to the Thr285 acceptor, positioning MCR as a mono-rather than multinuclear member of the alkaline phosphatase superfamily.",

author = "Emily Lythell and Emily Lythell and Reynier Suard{\'i}az and Reynier Suard{\'i}az and Reynier Suard{\'i}az and Reynier Suard{\'i}az and Philip Hinchliffe and Chonnikan Hanpaibool and Surawit Visitsatthawong and Oliveira, \{A. Sofia F.\} and Lang, \{Eric J.M.\} and Panida Surawatanawong and Lee, \{Vannajan Sanghiran\} and Thanyada Rungrotmongkol and Thanyada Rungrotmongkol and Natalie Fey and James Spencer and Mulholland, \{Adrian J.\}",

note = "Publisher Copyright: {\textcopyright} 2020 The Royal Society of Chemistry.",

year = "2020",

month = jun,

day = "25",

doi = "10.1039/d0cc02520h",

language = "English",

volume = "56",

pages = "6874--6877",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "50",

}

Lythell, E, Lythell, E, Suardíaz, R, Suardíaz, R, Suardíaz, R, Suardíaz, R, Hinchliffe, P, Hanpaibool, C, Visitsatthawong, S, Oliveira, ASF, Lang, EJM, Surawatanawong, P, Lee, VS, Rungrotmongkol, T, Rungrotmongkol, T, Fey, N, Spencer, J & Mulholland, AJ 2020, 'Resistance to the "last resort" antibiotic colistin: A single-zinc mechanism for phosphointermediate formation in MCR enzymes', Chemical Communications, vol. 56, no. 50, pp. 6874-6877. https://doi.org/10.1039/d0cc02520h

TY - JOUR

T1 - Resistance to the "last resort" antibiotic colistin

T2 - A single-zinc mechanism for phosphointermediate formation in MCR enzymes

AU - Lythell, Emily

AU - Suardíaz, Reynier

AU - Hinchliffe, Philip

AU - Hanpaibool, Chonnikan

AU - Visitsatthawong, Surawit

AU - Oliveira, A. Sofia F.

AU - Lang, Eric J.M.

AU - Surawatanawong, Panida

AU - Lee, Vannajan Sanghiran

AU - Rungrotmongkol, Thanyada

AU - Fey, Natalie

AU - Spencer, James

AU - Mulholland, Adrian J.

PY - 2020/6/25

Y1 - 2020/6/25

N2 - MCR (mobile colistin resistance) enzymes catalyse phosphoethanolamine (PEA) addition to bacterial lipid A, threatening the "last-resort"antibiotic colistin. Molecular dynamics and density functional theory simulations indicate that monozinc MCR supports PEA transfer to the Thr285 acceptor, positioning MCR as a mono-rather than multinuclear member of the alkaline phosphatase superfamily.

AB - MCR (mobile colistin resistance) enzymes catalyse phosphoethanolamine (PEA) addition to bacterial lipid A, threatening the "last-resort"antibiotic colistin. Molecular dynamics and density functional theory simulations indicate that monozinc MCR supports PEA transfer to the Thr285 acceptor, positioning MCR as a mono-rather than multinuclear member of the alkaline phosphatase superfamily.

UR - https://www.scopus.com/pages/publications/85087096464

U2 - 10.1039/d0cc02520h

DO - 10.1039/d0cc02520h

M3 - Article

C2 - 32432618

AN - SCOPUS:85087096464

SN - 1359-7345

VL - 56

SP - 6874

EP - 6877

JO - Chemical Communications

JF - Chemical Communications

IS - 50

ER -

Resistance to the "last resort" antibiotic colistin: A single-zinc mechanism for phosphointermediate formation in MCR enzymes

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