@inbook{5cb9c718173345ae8d93477e431cc5c2,
title = "Recombinant Production of Food Allergens in Yeast Pichia pastoris",
abstract = "The methylotrophic yeast Pichia pastoris (P. pastoris) is one of the expression systems widely used to produce recombinant heterologous proteins. In this chapter, the methodology to produce recombinant food allergens in P. pastoris is described. The methodology begins with the preparation of competent P. pastoris cells followed by the transformation of the competent cells by electroporation as well as the preparation of plasmid DNA for transformation. Moreover, the screening of yeast transformants by direct PCR to ensure integration of allergen DNA followed by small-scale expression of recombinant allergen in yeast cells is also described.",
keywords = "Food allergen, Food allergen DNA, Food allergen expression, Pichia pastoris expression, Recombinant allergen",
author = "Surapon Piboonpocanun",
note = "Publisher Copyright: {\textcopyright} 2024, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2024",
doi = "10.1007/978-1-0716-3453-0\_3",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "29--40",
booktitle = "Methods in Molecular Biology",
}