Recognition of deglycosylated larval proteins of Gnathostoma spinigerum by a monoclonal antibody and human gnathostomiasis antiserum

P. Uparanukraw; T. Harnnoi; A. Dantrakool; N. Morakote; W. Chaicumpa

doi:10.1017/s0022149x99000438

Recognition of deglycosylated larval proteins of Gnathostoma spinigerum by a monoclonal antibody and human gnathostomiasis antiserum

P. Uparanukraw
, T. Harnnoi
, A. Dantrakool
, N. Morakote
, W. Chaicumpa

Department of Parasitology

Faculty of Medicine, Chiang Mai University

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The study on the recognition of ³⁵S-labelled somatic antigens of Gnathostoma spinigerum advanced third-stage larva (aL3) has revealed that the mAb GN6/24 immunoprecipitated 26- and 24-kDa proteins from the undigested and N-glycosidase F-digested larval extracts, respectively. The recognition of the deglycosylated form of the glycoprotein indicated that the mAb reacted with the peptide epitope on the 26-kDa protein. Human gnathostomiasis antiserum immunoprecipitated most of the N-glycosidase F-digested larval proteins including the deglycosylated 26-kDa protein.

Original language	English
Pages (from-to)	273-276
Number of pages	4
Journal	Journal of Helminthology
Volume	73
Issue number	3
DOIs	https://doi.org/10.1017/s0022149x99000438
Publication status	Published - Sept 1999

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title = "Recognition of deglycosylated larval proteins of Gnathostoma spinigerum by a monoclonal antibody and human gnathostomiasis antiserum",

abstract = "The study on the recognition of 35S-labelled somatic antigens of Gnathostoma spinigerum advanced third-stage larva (aL3) has revealed that the mAb GN6/24 immunoprecipitated 26- and 24-kDa proteins from the undigested and N-glycosidase F-digested larval extracts, respectively. The recognition of the deglycosylated form of the glycoprotein indicated that the mAb reacted with the peptide epitope on the 26-kDa protein. Human gnathostomiasis antiserum immunoprecipitated most of the N-glycosidase F-digested larval proteins including the deglycosylated 26-kDa protein.",

author = "P. Uparanukraw and T. Harnnoi and A. Dantrakool and N. Morakote and W. Chaicumpa",

year = "1999",

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doi = "10.1017/s0022149x99000438",

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volume = "73",

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journal = "Journal of Helminthology",

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T1 - Recognition of deglycosylated larval proteins of Gnathostoma spinigerum by a monoclonal antibody and human gnathostomiasis antiserum

AU - Uparanukraw, P.

AU - Harnnoi, T.

AU - Dantrakool, A.

AU - Morakote, N.

AU - Chaicumpa, W.

PY - 1999/9

Y1 - 1999/9

N2 - The study on the recognition of 35S-labelled somatic antigens of Gnathostoma spinigerum advanced third-stage larva (aL3) has revealed that the mAb GN6/24 immunoprecipitated 26- and 24-kDa proteins from the undigested and N-glycosidase F-digested larval extracts, respectively. The recognition of the deglycosylated form of the glycoprotein indicated that the mAb reacted with the peptide epitope on the 26-kDa protein. Human gnathostomiasis antiserum immunoprecipitated most of the N-glycosidase F-digested larval proteins including the deglycosylated 26-kDa protein.

AB - The study on the recognition of 35S-labelled somatic antigens of Gnathostoma spinigerum advanced third-stage larva (aL3) has revealed that the mAb GN6/24 immunoprecipitated 26- and 24-kDa proteins from the undigested and N-glycosidase F-digested larval extracts, respectively. The recognition of the deglycosylated form of the glycoprotein indicated that the mAb reacted with the peptide epitope on the 26-kDa protein. Human gnathostomiasis antiserum immunoprecipitated most of the N-glycosidase F-digested larval proteins including the deglycosylated 26-kDa protein.

UR - https://www.scopus.com/pages/publications/0032859218

U2 - 10.1017/s0022149x99000438

DO - 10.1017/s0022149x99000438

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AN - SCOPUS:0032859218

SN - 0022-149X

VL - 73

SP - 273

EP - 276

JO - Journal of Helminthology

JF - Journal of Helminthology

IS - 3

ER -

Recognition of deglycosylated larval proteins of Gnathostoma spinigerum by a monoclonal antibody and human gnathostomiasis antiserum

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