Rapid immunofluorescence microscopy for diagnosis of melioidosis

Vanaporn Wuthiekanun; Varunee Desakorn; Gumphol Wongsuvan; Premjit Amornchai; Allen C. Cheng; Bina Maharjan; Direk Limmathurotsakul; Wirongrong Chierakul; Nicholas J. White; Nicholas P.J. Day; Sharon J. Peacock

doi:10.1128/CDLI.12.4.555-556.2005

Rapid immunofluorescence microscopy for diagnosis of melioidosis

Vanaporn Wuthiekanun
, Varunee Desakorn
, Gumphol Wongsuvan
, Premjit Amornchai
, Allen C. Cheng
, Bina Maharjan
, Direk Limmathurotsakul
, Wirongrong Chierakul
, Nicholas J. White
, Nicholas P.J. Day
, Sharon J. Peacock

Faculty of Tropical Medicine, Mahidol University
Menzies School of Health Research
Flinders University
Nuffield Department of Medicine

Research output: Contribution to journal › Article › peer-review

66 Citations (Scopus)

Abstract

An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were prospectively compared with the culture results for 776 specimens from patients with suspected melioidosis. The sensitivities of both IF tests were 66%, and the specificities were 99.5 and 99.4%, respectively.

Original language	English
Pages (from-to)	555-556
Number of pages	2
Journal	Clinical and Diagnostic Laboratory Immunology
Volume	12
Issue number	4
DOIs	https://doi.org/10.1128/CDLI.12.4.555-556.2005
Publication status	Published - Apr 2005

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10.1128/CDLI.12.4.555-556.2005

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title = "Rapid immunofluorescence microscopy for diagnosis of melioidosis",

abstract = "An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were prospectively compared with the culture results for 776 specimens from patients with suspected melioidosis. The sensitivities of both IF tests were 66\%, and the specificities were 99.5 and 99.4\%, respectively.",

author = "Vanaporn Wuthiekanun and Varunee Desakorn and Gumphol Wongsuvan and Premjit Amornchai and Cheng, \{Allen C.\} and Bina Maharjan and Direk Limmathurotsakul and Wirongrong Chierakul and White, \{Nicholas J.\} and Day, \{Nicholas P.J.\} and Peacock, \{Sharon J.\}",

year = "2005",

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doi = "10.1128/CDLI.12.4.555-556.2005",

language = "English",

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T1 - Rapid immunofluorescence microscopy for diagnosis of melioidosis

AU - Wuthiekanun, Vanaporn

AU - Desakorn, Varunee

AU - Wongsuvan, Gumphol

AU - Amornchai, Premjit

AU - Cheng, Allen C.

AU - Maharjan, Bina

AU - Limmathurotsakul, Direk

AU - Chierakul, Wirongrong

AU - White, Nicholas J.

AU - Day, Nicholas P.J.

AU - Peacock, Sharon J.

PY - 2005/4

Y1 - 2005/4

N2 - An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were prospectively compared with the culture results for 776 specimens from patients with suspected melioidosis. The sensitivities of both IF tests were 66%, and the specificities were 99.5 and 99.4%, respectively.

AB - An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were prospectively compared with the culture results for 776 specimens from patients with suspected melioidosis. The sensitivities of both IF tests were 66%, and the specificities were 99.5 and 99.4%, respectively.

UR - https://www.scopus.com/pages/publications/22844445046

U2 - 10.1128/CDLI.12.4.555-556.2005

DO - 10.1128/CDLI.12.4.555-556.2005

M3 - Article

C2 - 15817767

AN - SCOPUS:22844445046

SN - 1071-412X

VL - 12

SP - 555

EP - 556

JO - Clinical and Diagnostic Laboratory Immunology

JF - Clinical and Diagnostic Laboratory Immunology

IS - 4

ER -

Rapid immunofluorescence microscopy for diagnosis of melioidosis

Abstract

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