Quantitation of human immunodeficiency virus type 1 DNA by competitive nested PCR assay

K. Sangsiriwut; P. Auewarakul; S. Suwanagool; C. Wasi

Quantitation of human immunodeficiency virus type 1 DNA by competitive nested PCR assay

K. Sangsiriwut
, P. Auewarakul
, S. Suwanagool
, C. Wasi

Department of Microbiology

Siriraj Hospital

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

A quantitative competitive nested PCR assay was developed for quantifying HIV-1 proviral DNA in clinical samples. A competitor DNA was constructed from a conserved region of the HIV-1 gag gene by deleting a sequence of 18 base pairs. We quantitated HIV-1 proviral DNA copy number in clinical samples. Peripheral blood mononuclear cells (PBMCs) from 35 HIV-infected patients with a CD₄ count rage of 4-728 cell/mm³ were analyzed by this method. The copy numbers of HIV-1 DNA detected ranged between 518 to 67,340 copies per 10⁶ CD₄⁺ T-cells. The copy numbers correlated inversely with the CD₄ counts.

Original language	English
Pages (from-to)	43-48
Number of pages	6
Journal	Asian Pacific Journal of Allergy and Immunology
Volume	19
Issue number	1
Publication status	Published - 2001

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SDG 3 Good Health and Well-being

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abstract = "A quantitative competitive nested PCR assay was developed for quantifying HIV-1 proviral DNA in clinical samples. A competitor DNA was constructed from a conserved region of the HIV-1 gag gene by deleting a sequence of 18 base pairs. We quantitated HIV-1 proviral DNA copy number in clinical samples. Peripheral blood mononuclear cells (PBMCs) from 35 HIV-infected patients with a CD4 count rage of 4-728 cell/mm3 were analyzed by this method. The copy numbers of HIV-1 DNA detected ranged between 518 to 67,340 copies per 106 CD4+ T-cells. The copy numbers correlated inversely with the CD4 counts.",

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AU - Sangsiriwut, K.

AU - Auewarakul, P.

AU - Suwanagool, S.

AU - Wasi, C.

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AB - A quantitative competitive nested PCR assay was developed for quantifying HIV-1 proviral DNA in clinical samples. A competitor DNA was constructed from a conserved region of the HIV-1 gag gene by deleting a sequence of 18 base pairs. We quantitated HIV-1 proviral DNA copy number in clinical samples. Peripheral blood mononuclear cells (PBMCs) from 35 HIV-infected patients with a CD4 count rage of 4-728 cell/mm3 were analyzed by this method. The copy numbers of HIV-1 DNA detected ranged between 518 to 67,340 copies per 106 CD4+ T-cells. The copy numbers correlated inversely with the CD4 counts.

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IS - 1

ER -

Quantitation of human immunodeficiency virus type 1 DNA by competitive nested PCR assay

Abstract

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