TY - CHAP
T1 - Protocol for a facile multiplex PCR for multi-antimicrobial resistance and gonococcus detection
AU - Lawung, Ratana
AU - Cherdtrakulkiat, Rungrot
AU - Prachayasittikul, Virapong
PY - 2012
Y1 - 2012
N2 - Gonorrhea is a continuing problem worldwide particularly in terms of the spread of multiple drug resistance. We have successfully developed an efficient PCR method for the simultaneous identification of gonococci and detection of the antimicrobial-resistant profile. By this method, penicillinase-producing Neisseria gonorrhoeae (PPNG), high-level tetracycline-resistant N. gonorrhoeae (TRNG), and ciprofloxacin-resistant N. gonorrhoeae (CRNG) can be clearly identified. Moreover, the plasmid-types of penicillin and tetracycline resistance are also characterized. The method has 100 % sensitivity and specificity. It is also time-and labor-saving compared to the conventional method. Thus, the procedure is suitable for epidemiological surveillance.
AB - Gonorrhea is a continuing problem worldwide particularly in terms of the spread of multiple drug resistance. We have successfully developed an efficient PCR method for the simultaneous identification of gonococci and detection of the antimicrobial-resistant profile. By this method, penicillinase-producing Neisseria gonorrhoeae (PPNG), high-level tetracycline-resistant N. gonorrhoeae (TRNG), and ciprofloxacin-resistant N. gonorrhoeae (CRNG) can be clearly identified. Moreover, the plasmid-types of penicillin and tetracycline resistance are also characterized. The method has 100 % sensitivity and specificity. It is also time-and labor-saving compared to the conventional method. Thus, the procedure is suitable for epidemiological surveillance.
KW - CRNG (ciprofloxacin-resistant N. gonorrhoeae)
KW - Multiple antimicrobial resistances
KW - Multiplex PCR
KW - Neisseria gonorrhoeae
KW - PPNG (penicillinase-producing N. gonorrhoeae)
KW - TRNG (tetracycline-resistant N. gonorrhoeae)
UR - https://www.scopus.com/pages/publications/84864858382
U2 - 10.1007/978-1-61779-937-2_23
DO - 10.1007/978-1-61779-937-2_23
M3 - Chapter
C2 - 22782830
AN - SCOPUS:84864858382
SN - 9781617799365
T3 - Methods in Molecular Biology
SP - 329
EP - 341
BT - Diagnosis of Sexually Transmitted Diseases
A2 - MacKenzie, Colin
A2 - Henrich, Birgit
ER -