Optimization of culture of Leptospira from humans with leptospirosis

Vanaporn Wuthiekanun; Wirongrong Chierakul; Direk Limmathurotsakul; Lee D. Smythe; Meegan L. Symonds; Michael F. Dohnt; Andrew T. Slack; Roongreung Limpaiboon; Yupin Suputtamongkol; Nicholas J. White; Nicholas P.J. Day; Sharon J. Peacock

doi:10.1128/JCM.02430-06

Optimization of culture of Leptospira from humans with leptospirosis

Vanaporn Wuthiekanun
, Wirongrong Chierakul
, Direk Limmathurotsakul
, Lee D. Smythe
, Meegan L. Symonds
, Michael F. Dohnt
, Andrew T. Slack
, Roongreung Limpaiboon
, Yupin Suputtamongkol
, Nicholas J. White
, Nicholas P.J. Day
, Sharon J. Peacock

Mahidol University
Faculty of Tropical Medicine, Mahidol University
Queensland Health
Udon Thani Regional Hospital
Nuffield Department of Medicine

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

A prospective study of 989 patients with acute febrile illness was performed in northeast Thailand to define the yield of Leptospira from four different types of blood sample. Based on a comparison of the yields from whole blood, surface plasma, deposit from spun plasma, and dotted blood samples from 80 patients with culture-proven leptospirosis, we suggest a sampling strategy in which culture is performed using whole blood and deposit from spun plasma.

Original language	English
Pages (from-to)	1363-1365
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	45
Issue number	4
DOIs	https://doi.org/10.1128/JCM.02430-06
Publication status	Published - Apr 2007

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title = "Optimization of culture of Leptospira from humans with leptospirosis",

abstract = "A prospective study of 989 patients with acute febrile illness was performed in northeast Thailand to define the yield of Leptospira from four different types of blood sample. Based on a comparison of the yields from whole blood, surface plasma, deposit from spun plasma, and dotted blood samples from 80 patients with culture-proven leptospirosis, we suggest a sampling strategy in which culture is performed using whole blood and deposit from spun plasma.",

author = "Vanaporn Wuthiekanun and Wirongrong Chierakul and Direk Limmathurotsakul and Smythe, \{Lee D.\} and Symonds, \{Meegan L.\} and Dohnt, \{Michael F.\} and Slack, \{Andrew T.\} and Roongreung Limpaiboon and Yupin Suputtamongkol and White, \{Nicholas J.\} and Day, \{Nicholas P.J.\} and Peacock, \{Sharon J.\}",

year = "2007",

month = apr,

doi = "10.1128/JCM.02430-06",

language = "English",

volume = "45",

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T1 - Optimization of culture of Leptospira from humans with leptospirosis

AU - Wuthiekanun, Vanaporn

AU - Chierakul, Wirongrong

AU - Limmathurotsakul, Direk

AU - Smythe, Lee D.

AU - Symonds, Meegan L.

AU - Dohnt, Michael F.

AU - Slack, Andrew T.

AU - Limpaiboon, Roongreung

AU - Suputtamongkol, Yupin

AU - White, Nicholas J.

AU - Day, Nicholas P.J.

AU - Peacock, Sharon J.

PY - 2007/4

Y1 - 2007/4

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AB - A prospective study of 989 patients with acute febrile illness was performed in northeast Thailand to define the yield of Leptospira from four different types of blood sample. Based on a comparison of the yields from whole blood, surface plasma, deposit from spun plasma, and dotted blood samples from 80 patients with culture-proven leptospirosis, we suggest a sampling strategy in which culture is performed using whole blood and deposit from spun plasma.

UR - https://www.scopus.com/pages/publications/34247263899

U2 - 10.1128/JCM.02430-06

DO - 10.1128/JCM.02430-06

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AN - SCOPUS:34247263899

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VL - 45

SP - 1363

EP - 1365

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 4

ER -

Optimization of culture of Leptospira from humans with leptospirosis

Abstract

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