On-chip irreversible electroporation for bacterial cell membrane rupture

S. Jaikla; T. Maturos; T. Pogfay; C. Neatpisarnvanit; P. Sritongkham; A. Tuantranont

doi:10.1109/BMEiCon.2012.6465456

On-chip irreversible electroporation for bacterial cell membrane rupture

S. Jaikla
, T. Maturos
, T. Pogfay
, C. Neatpisarnvanit
, P. Sritongkham
, A. Tuantranont

Department of Electrical Engineering

Mahidol University
National Electronics and Computer Technology Center

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Citations (Scopus)

Abstract

In this work, the DNA preparation process for genetic analysis was developed. To rupture the cell membrane of Salmonella on a microfluidic chip, high electric field pulses were applied through gold electrode. The applied voltage was less than 10V DC voltage. Electric field pulses were short-duration in microsecond. Fluorescent spectrophotometer was employed for live/dead bacterial cells detection. The live bacterial cells were tagged by SYTO 9 green fluorescent stain. The red-fluorescent nucleic acid stain, propidium iodide was used to tag the dead cells. The highest percentage of 95% dead cells was at 7V applied voltage and 90 μs pulse duration. This result was confirmed by plate count with completely dead cell.

Original language	English
Title of host publication	5th 2012 Biomedical Engineering International Conference, BMEiCON 2012
DOIs	https://doi.org/10.1109/BMEiCon.2012.6465456
Publication status	Published - 2012
Event	5th 2012 Biomedical Engineering International Conference, BMEiCON 2012 - Muang, Ubon Ratchathani, Thailand Duration: 5 Dec 2012 → 7 Dec 2012

Publication series

Name	5th 2012 Biomedical Engineering International Conference, BMEiCON 2012

Conference

Conference	5th 2012 Biomedical Engineering International Conference, BMEiCON 2012
Country/Territory	Thailand
City	Muang, Ubon Ratchathani
Period	5/12/12 → 7/12/12

Keywords

Irreversible electroporation
Microfluidics
Pulsed electric field

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10.1109/BMEiCon.2012.6465456

Cite this

Jaikla, S., Maturos, T., Pogfay, T., Neatpisarnvanit, C., Sritongkham, P., & Tuantranont, A. (2012). On-chip irreversible electroporation for bacterial cell membrane rupture. In 5th 2012 Biomedical Engineering International Conference, BMEiCON 2012 Article 6465456 (5th 2012 Biomedical Engineering International Conference, BMEiCON 2012). https://doi.org/10.1109/BMEiCon.2012.6465456

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title = "On-chip irreversible electroporation for bacterial cell membrane rupture",

abstract = "In this work, the DNA preparation process for genetic analysis was developed. To rupture the cell membrane of Salmonella on a microfluidic chip, high electric field pulses were applied through gold electrode. The applied voltage was less than 10V DC voltage. Electric field pulses were short-duration in microsecond. Fluorescent spectrophotometer was employed for live/dead bacterial cells detection. The live bacterial cells were tagged by SYTO 9 green fluorescent stain. The red-fluorescent nucleic acid stain, propidium iodide was used to tag the dead cells. The highest percentage of 95\% dead cells was at 7V applied voltage and 90 μs pulse duration. This result was confirmed by plate count with completely dead cell.",

keywords = "Irreversible electroporation, Microfluidics, Pulsed electric field",

author = "S. Jaikla and T. Maturos and T. Pogfay and C. Neatpisarnvanit and P. Sritongkham and A. Tuantranont",

year = "2012",

doi = "10.1109/BMEiCon.2012.6465456",

language = "English",

isbn = "9781467348928",

series = "5th 2012 Biomedical Engineering International Conference, BMEiCON 2012",

booktitle = "5th 2012 Biomedical Engineering International Conference, BMEiCON 2012",

note = "5th 2012 Biomedical Engineering International Conference, BMEiCON 2012 ; Conference date: 05-12-2012 Through 07-12-2012",

}

Jaikla, S, Maturos, T, Pogfay, T, Neatpisarnvanit, C, Sritongkham, P & Tuantranont, A 2012, On-chip irreversible electroporation for bacterial cell membrane rupture. in 5th 2012 Biomedical Engineering International Conference, BMEiCON 2012., 6465456, 5th 2012 Biomedical Engineering International Conference, BMEiCON 2012, 5th 2012 Biomedical Engineering International Conference, BMEiCON 2012, Muang, Ubon Ratchathani, Thailand, 5/12/12. https://doi.org/10.1109/BMEiCon.2012.6465456

On-chip irreversible electroporation for bacterial cell membrane rupture. / Jaikla, S.; Maturos, T.; Pogfay, T. et al.
5th 2012 Biomedical Engineering International Conference, BMEiCON 2012. 2012. 6465456 (5th 2012 Biomedical Engineering International Conference, BMEiCON 2012).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - On-chip irreversible electroporation for bacterial cell membrane rupture

AU - Jaikla, S.

AU - Maturos, T.

AU - Pogfay, T.

AU - Neatpisarnvanit, C.

AU - Sritongkham, P.

AU - Tuantranont, A.

PY - 2012

Y1 - 2012

N2 - In this work, the DNA preparation process for genetic analysis was developed. To rupture the cell membrane of Salmonella on a microfluidic chip, high electric field pulses were applied through gold electrode. The applied voltage was less than 10V DC voltage. Electric field pulses were short-duration in microsecond. Fluorescent spectrophotometer was employed for live/dead bacterial cells detection. The live bacterial cells were tagged by SYTO 9 green fluorescent stain. The red-fluorescent nucleic acid stain, propidium iodide was used to tag the dead cells. The highest percentage of 95% dead cells was at 7V applied voltage and 90 μs pulse duration. This result was confirmed by plate count with completely dead cell.

AB - In this work, the DNA preparation process for genetic analysis was developed. To rupture the cell membrane of Salmonella on a microfluidic chip, high electric field pulses were applied through gold electrode. The applied voltage was less than 10V DC voltage. Electric field pulses were short-duration in microsecond. Fluorescent spectrophotometer was employed for live/dead bacterial cells detection. The live bacterial cells were tagged by SYTO 9 green fluorescent stain. The red-fluorescent nucleic acid stain, propidium iodide was used to tag the dead cells. The highest percentage of 95% dead cells was at 7V applied voltage and 90 μs pulse duration. This result was confirmed by plate count with completely dead cell.

KW - Irreversible electroporation

KW - Microfluidics

KW - Pulsed electric field

UR - https://www.scopus.com/pages/publications/84875086374

U2 - 10.1109/BMEiCon.2012.6465456

DO - 10.1109/BMEiCon.2012.6465456

M3 - Conference contribution

AN - SCOPUS:84875086374

SN - 9781467348928

T3 - 5th 2012 Biomedical Engineering International Conference, BMEiCON 2012

BT - 5th 2012 Biomedical Engineering International Conference, BMEiCON 2012

T2 - 5th 2012 Biomedical Engineering International Conference, BMEiCON 2012

Y2 - 5 December 2012 through 7 December 2012

ER -

On-chip irreversible electroporation for bacterial cell membrane rupture

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Keywords

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