Nitric oxide and tumor necrosis factor-alpha inhibitory substances from the rhizomes of Kaempferia marginata

Kanidta Kaewkroek; Chatchai Wattanapiromsakul; Palangpon Kongsaeree; Supinya Tewtrakul

doi:10.1177/1934578x1300800904

Nitric oxide and tumor necrosis factor-alpha inhibitory substances from the rhizomes of Kaempferia marginata

Kanidta Kaewkroek
, Chatchai Wattanapiromsakul
, Palangpon Kongsaeree
, Supinya Tewtrakul

Prince of Songkla University
Mahidol University

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The ethanol extract of the rhizomes of Kaempferia marginata showed a potent inhibitory effect against lipopolysaccharide (LPS)-induced nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α) release in RAW264.7 cells. Moreover, the partition with various organic solvents also inhibited NO production. One new pimarane-type diterpene, 1α- acetoxysandaracopimaradien-2α-ol (5), along with four known diterpenes (1-4), were isolated from the n-hexane and chloroform layers, respectively. Among these metabolites, compounds 1 and 4 were isolated for the first time from K. marginata. Compounds 1-5 showed significant inhibitory effects on NO production, with IC₅₀ values ranging from 38.6 to 51.9 μM. Furthermore, compound 2 also exhibited significant activity against TNF-α release (1C₅₀ = 48.3 μM). These findings may support the use of K. marginata by traditional doctors for treatment of inflammatory-related diseases.

Original language	English
Pages (from-to)	1205-1208
Number of pages	4
Journal	Natural Product Communications
Volume	8
Issue number	9
DOIs	https://doi.org/10.1177/1934578x1300800904
Publication status	Published - Sept 2013
Externally published	Yes

Keywords

Kaempferia marginata
Nitric oxide
Tumor necrosis factor-α
Zingiberaceae
mRNA expression

Access to Document

10.1177/1934578x1300800904

Cite this

@article{51db7deaf2cd4cc6be8035a340f4c35d,

title = "Nitric oxide and tumor necrosis factor-alpha inhibitory substances from the rhizomes of Kaempferia marginata",

abstract = "The ethanol extract of the rhizomes of Kaempferia marginata showed a potent inhibitory effect against lipopolysaccharide (LPS)-induced nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α) release in RAW264.7 cells. Moreover, the partition with various organic solvents also inhibited NO production. One new pimarane-type diterpene, 1α- acetoxysandaracopimaradien-2α-ol (5), along with four known diterpenes (1-4), were isolated from the n-hexane and chloroform layers, respectively. Among these metabolites, compounds 1 and 4 were isolated for the first time from K. marginata. Compounds 1-5 showed significant inhibitory effects on NO production, with IC50 values ranging from 38.6 to 51.9 μM. Furthermore, compound 2 also exhibited significant activity against TNF-α release (1C50 = 48.3 μM). These findings may support the use of K. marginata by traditional doctors for treatment of inflammatory-related diseases.",

keywords = "Kaempferia marginata, Nitric oxide, Tumor necrosis factor-α, Zingiberaceae, mRNA expression",

author = "Kanidta Kaewkroek and Chatchai Wattanapiromsakul and Palangpon Kongsaeree and Supinya Tewtrakul",

year = "2013",

month = sep,

doi = "10.1177/1934578x1300800904",

language = "English",

volume = "8",

pages = "1205--1208",

journal = "Natural Product Communications",

issn = "1934-578X",

publisher = "SAGE Publications Inc.",

number = "9",

}

TY - JOUR

T1 - Nitric oxide and tumor necrosis factor-alpha inhibitory substances from the rhizomes of Kaempferia marginata

AU - Kaewkroek, Kanidta

AU - Wattanapiromsakul, Chatchai

AU - Kongsaeree, Palangpon

AU - Tewtrakul, Supinya

PY - 2013/9

Y1 - 2013/9

N2 - The ethanol extract of the rhizomes of Kaempferia marginata showed a potent inhibitory effect against lipopolysaccharide (LPS)-induced nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α) release in RAW264.7 cells. Moreover, the partition with various organic solvents also inhibited NO production. One new pimarane-type diterpene, 1α- acetoxysandaracopimaradien-2α-ol (5), along with four known diterpenes (1-4), were isolated from the n-hexane and chloroform layers, respectively. Among these metabolites, compounds 1 and 4 were isolated for the first time from K. marginata. Compounds 1-5 showed significant inhibitory effects on NO production, with IC50 values ranging from 38.6 to 51.9 μM. Furthermore, compound 2 also exhibited significant activity against TNF-α release (1C50 = 48.3 μM). These findings may support the use of K. marginata by traditional doctors for treatment of inflammatory-related diseases.

AB - The ethanol extract of the rhizomes of Kaempferia marginata showed a potent inhibitory effect against lipopolysaccharide (LPS)-induced nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α) release in RAW264.7 cells. Moreover, the partition with various organic solvents also inhibited NO production. One new pimarane-type diterpene, 1α- acetoxysandaracopimaradien-2α-ol (5), along with four known diterpenes (1-4), were isolated from the n-hexane and chloroform layers, respectively. Among these metabolites, compounds 1 and 4 were isolated for the first time from K. marginata. Compounds 1-5 showed significant inhibitory effects on NO production, with IC50 values ranging from 38.6 to 51.9 μM. Furthermore, compound 2 also exhibited significant activity against TNF-α release (1C50 = 48.3 μM). These findings may support the use of K. marginata by traditional doctors for treatment of inflammatory-related diseases.

KW - Kaempferia marginata

KW - Nitric oxide

KW - Tumor necrosis factor-α

KW - Zingiberaceae

KW - mRNA expression

UR - https://www.scopus.com/pages/publications/84886815888

U2 - 10.1177/1934578x1300800904

DO - 10.1177/1934578x1300800904

M3 - Article

C2 - 24273846

AN - SCOPUS:84886815888

SN - 1934-578X

VL - 8

SP - 1205

EP - 1208

JO - Natural Product Communications

JF - Natural Product Communications

IS - 9

ER -

Nitric oxide and tumor necrosis factor-alpha inhibitory substances from the rhizomes of Kaempferia marginata

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this