Newly established process combining partial hydrogenotrophic denitrification and anammox for nitrogen removal

Kenta Shinoda; Rawintra Eamrat; Yuya Tsutsumi; Suphatchai Rujakom; Tippawan Singhopon; Tatsuru Kamei; Futaba Kazama

doi:10.2166/wst.2020.406

Newly established process combining partial hydrogenotrophic denitrification and anammox for nitrogen removal

Kenta Shinoda
, Rawintra Eamrat
, Yuya Tsutsumi
, Suphatchai Rujakom
, Tippawan Singhopon
, Tatsuru Kamei
, Futaba Kazama

University of Yamanashi
Ramkhamhaeng University

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The anaerobic ammonium oxidation (anammox) process holds great promise for treating nitrogen-contaminated water; stable nitrite-nitrogen (NO ₂ -N) production is significant to anammox performance. In this study, partial hydrogenotrophic denitrification (PHD) was used to stably and efficiently produce NO ₂ -N from nitrate-nitrogen (NO ₃ -N). An investigation of the effects of initial pH on the PHD process revealed that a high NO ₂ -N production efficiency (77.9%) could be ensured by setting an initial pH of 10.5. A combined PHD-anammox process was run for more than three months with maximal ammonium-nitrogen (NH^þ₄ -N), NO ₃ -N, and total dissolved inorganic nitrogen removal efficiencies of 93.4, 98.0, and 86.9%, respectively. The NO ₂ -N to NH^þ₄ -N and NO ₃ -N to NH^þ₄ -N ratios indicated that various bioprocesses were involved in nitrogen removal during the anammox stage, and a 16S rRNA gene amplicon sequencing was performed to further clarify the composition of microbial communities and mechanisms involved in the nitrogen removal process.

Original language	English
Pages (from-to)	1272-1284
Number of pages	13
Journal	Water Science and Technology
Volume	82
Issue number	7
DOIs	https://doi.org/10.2166/wst.2020.406
Publication status	Published - 1 Oct 2020
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 6 Clean Water and Sanitation

Keywords

Anammox
Double stage
High pH
Hydrogenotrophic denitrification
Microbial community
Nitrite accumulation

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10.2166/wst.2020.406

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title = "Newly established process combining partial hydrogenotrophic denitrification and anammox for nitrogen removal",

abstract = "The anaerobic ammonium oxidation (anammox) process holds great promise for treating nitrogen-contaminated water; stable nitrite-nitrogen (NO 2 -N) production is significant to anammox performance. In this study, partial hydrogenotrophic denitrification (PHD) was used to stably and efficiently produce NO 2 -N from nitrate-nitrogen (NO 3 -N). An investigation of the effects of initial pH on the PHD process revealed that a high NO 2 -N production efficiency (77.9\%) could be ensured by setting an initial pH of 10.5. A combined PHD-anammox process was run for more than three months with maximal ammonium-nitrogen (NH{\th}4 -N), NO 3 -N, and total dissolved inorganic nitrogen removal efficiencies of 93.4, 98.0, and 86.9\%, respectively. The NO 2 -N to NH{\th}4 -N and NO 3 -N to NH{\th}4 -N ratios indicated that various bioprocesses were involved in nitrogen removal during the anammox stage, and a 16S rRNA gene amplicon sequencing was performed to further clarify the composition of microbial communities and mechanisms involved in the nitrogen removal process.",

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author = "Kenta Shinoda and Rawintra Eamrat and Yuya Tsutsumi and Suphatchai Rujakom and Tippawan Singhopon and Tatsuru Kamei and Futaba Kazama",

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T1 - Newly established process combining partial hydrogenotrophic denitrification and anammox for nitrogen removal

AU - Shinoda, Kenta

AU - Eamrat, Rawintra

AU - Tsutsumi, Yuya

AU - Rujakom, Suphatchai

AU - Singhopon, Tippawan

AU - Kamei, Tatsuru

AU - Kazama, Futaba

PY - 2020/10/1

Y1 - 2020/10/1

N2 - The anaerobic ammonium oxidation (anammox) process holds great promise for treating nitrogen-contaminated water; stable nitrite-nitrogen (NO 2 -N) production is significant to anammox performance. In this study, partial hydrogenotrophic denitrification (PHD) was used to stably and efficiently produce NO 2 -N from nitrate-nitrogen (NO 3 -N). An investigation of the effects of initial pH on the PHD process revealed that a high NO 2 -N production efficiency (77.9%) could be ensured by setting an initial pH of 10.5. A combined PHD-anammox process was run for more than three months with maximal ammonium-nitrogen (NHþ4 -N), NO 3 -N, and total dissolved inorganic nitrogen removal efficiencies of 93.4, 98.0, and 86.9%, respectively. The NO 2 -N to NHþ4 -N and NO 3 -N to NHþ4 -N ratios indicated that various bioprocesses were involved in nitrogen removal during the anammox stage, and a 16S rRNA gene amplicon sequencing was performed to further clarify the composition of microbial communities and mechanisms involved in the nitrogen removal process.

AB - The anaerobic ammonium oxidation (anammox) process holds great promise for treating nitrogen-contaminated water; stable nitrite-nitrogen (NO 2 -N) production is significant to anammox performance. In this study, partial hydrogenotrophic denitrification (PHD) was used to stably and efficiently produce NO 2 -N from nitrate-nitrogen (NO 3 -N). An investigation of the effects of initial pH on the PHD process revealed that a high NO 2 -N production efficiency (77.9%) could be ensured by setting an initial pH of 10.5. A combined PHD-anammox process was run for more than three months with maximal ammonium-nitrogen (NHþ4 -N), NO 3 -N, and total dissolved inorganic nitrogen removal efficiencies of 93.4, 98.0, and 86.9%, respectively. The NO 2 -N to NHþ4 -N and NO 3 -N to NHþ4 -N ratios indicated that various bioprocesses were involved in nitrogen removal during the anammox stage, and a 16S rRNA gene amplicon sequencing was performed to further clarify the composition of microbial communities and mechanisms involved in the nitrogen removal process.

KW - Anammox

KW - Double stage

KW - High pH

KW - Hydrogenotrophic denitrification

KW - Microbial community

KW - Nitrite accumulation

UR - https://www.scopus.com/pages/publications/85094219684

U2 - 10.2166/wst.2020.406

DO - 10.2166/wst.2020.406

M3 - Article

C2 - 33079708

AN - SCOPUS:85094219684

SN - 0273-1223

VL - 82

SP - 1272

EP - 1284

JO - Water Science and Technology

JF - Water Science and Technology

IS - 7

ER -

Newly established process combining partial hydrogenotrophic denitrification and anammox for nitrogen removal

Abstract

UN SDGs

Keywords

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