Morinda citrifolia leaves enhance osteogenic differentiation and mineralization of human periodontal ligament cells

Kanitsak Boonanantanasarn; Kajohnkiart Janebodin; Prapan Suppakpatana; Tawepong Arayapisit; Jit aree Rodsutthi; Panjit Chunhabundit; Surintorn Boonanuntanasarn; Wanida Sripairojthikoon

doi:10.4012/dmj.2012-053

Morinda citrifolia leaves enhance osteogenic differentiation and mineralization of human periodontal ligament cells

Kanitsak Boonanantanasarn
, Kajohnkiart Janebodin
, Prapan Suppakpatana
, Tawepong Arayapisit
, Jit aree Rodsutthi
, Panjit Chunhabundit
, Surintorn Boonanuntanasarn
, Wanida Sripairojthikoon

Department of Anatomy

Mahidol University, Faculty of Dentistry
University at Buffalo, The State University of New York
Suranaree University of Technology

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

This present study investigated the potential of Morinda citrifolia leaf aqueous extract to induce osteogenic differentiation and matrix mineralization of human periodontal ligament (hPDL) cells. Human periodontal ligament cells were cultured in complete medium, ascorbic acid with β-glycerophosphate, or Morinda citrifolia leaf aqueous extract. Morinda citrifolia leaf aqueous extract significantly increased alkaline phosphatase activity compared to culturing in complete medium or ascorbic acid with β-glycerophosphate. Matrixcontaining mineralized nodules were formed only when the cells were cultured in the presence of Morinda citrifolia leaf aqueous extract. These nodules showed positive alizarin red S staining and were rich in calcium and phosphorus according to energy dispersive X-ray analysis. In conclusion, Morinda citrifolia leaf extract promoted osteogenic differentiation and matrix mineralization in human periodontal ligament cells, a clear indication of the therapeutic potential of Morinda citrifolia leaves in bone and periodontal tissue regeneration.

Original language	English
Pages (from-to)	863-871
Number of pages	9
Journal	Dental Materials Journal
Volume	31
Issue number	5
DOIs	https://doi.org/10.4012/dmj.2012-053
Publication status	Published - 2012

Keywords

Mineralization
Morinda citrifolia
Osteogenic differentiation
Periodontal ligament cells

Access to Document

10.4012/dmj.2012-053

Cite this

@article{13b9673f657f4dd2a24f5f8797b31d9f,

title = "Morinda citrifolia leaves enhance osteogenic differentiation and mineralization of human periodontal ligament cells",

abstract = "This present study investigated the potential of Morinda citrifolia leaf aqueous extract to induce osteogenic differentiation and matrix mineralization of human periodontal ligament (hPDL) cells. Human periodontal ligament cells were cultured in complete medium, ascorbic acid with β-glycerophosphate, or Morinda citrifolia leaf aqueous extract. Morinda citrifolia leaf aqueous extract significantly increased alkaline phosphatase activity compared to culturing in complete medium or ascorbic acid with β-glycerophosphate. Matrixcontaining mineralized nodules were formed only when the cells were cultured in the presence of Morinda citrifolia leaf aqueous extract. These nodules showed positive alizarin red S staining and were rich in calcium and phosphorus according to energy dispersive X-ray analysis. In conclusion, Morinda citrifolia leaf extract promoted osteogenic differentiation and matrix mineralization in human periodontal ligament cells, a clear indication of the therapeutic potential of Morinda citrifolia leaves in bone and periodontal tissue regeneration.",

keywords = "Mineralization, Morinda citrifolia, Osteogenic differentiation, Periodontal ligament cells",

author = "Kanitsak Boonanantanasarn and Kajohnkiart Janebodin and Prapan Suppakpatana and Tawepong Arayapisit and Rodsutthi, \{Jit aree\} and Panjit Chunhabundit and Surintorn Boonanuntanasarn and Wanida Sripairojthikoon",

year = "2012",

doi = "10.4012/dmj.2012-053",

language = "English",

volume = "31",

pages = "863--871",

journal = "Dental Materials Journal",

issn = "0287-4547",

publisher = "Japanese Society for Dental Materials and Devices",

number = "5",

}

TY - JOUR

T1 - Morinda citrifolia leaves enhance osteogenic differentiation and mineralization of human periodontal ligament cells

AU - Boonanantanasarn, Kanitsak

AU - Janebodin, Kajohnkiart

AU - Suppakpatana, Prapan

AU - Arayapisit, Tawepong

AU - Rodsutthi, Jit aree

AU - Chunhabundit, Panjit

AU - Boonanuntanasarn, Surintorn

AU - Sripairojthikoon, Wanida

PY - 2012

Y1 - 2012

N2 - This present study investigated the potential of Morinda citrifolia leaf aqueous extract to induce osteogenic differentiation and matrix mineralization of human periodontal ligament (hPDL) cells. Human periodontal ligament cells were cultured in complete medium, ascorbic acid with β-glycerophosphate, or Morinda citrifolia leaf aqueous extract. Morinda citrifolia leaf aqueous extract significantly increased alkaline phosphatase activity compared to culturing in complete medium or ascorbic acid with β-glycerophosphate. Matrixcontaining mineralized nodules were formed only when the cells were cultured in the presence of Morinda citrifolia leaf aqueous extract. These nodules showed positive alizarin red S staining and were rich in calcium and phosphorus according to energy dispersive X-ray analysis. In conclusion, Morinda citrifolia leaf extract promoted osteogenic differentiation and matrix mineralization in human periodontal ligament cells, a clear indication of the therapeutic potential of Morinda citrifolia leaves in bone and periodontal tissue regeneration.

AB - This present study investigated the potential of Morinda citrifolia leaf aqueous extract to induce osteogenic differentiation and matrix mineralization of human periodontal ligament (hPDL) cells. Human periodontal ligament cells were cultured in complete medium, ascorbic acid with β-glycerophosphate, or Morinda citrifolia leaf aqueous extract. Morinda citrifolia leaf aqueous extract significantly increased alkaline phosphatase activity compared to culturing in complete medium or ascorbic acid with β-glycerophosphate. Matrixcontaining mineralized nodules were formed only when the cells were cultured in the presence of Morinda citrifolia leaf aqueous extract. These nodules showed positive alizarin red S staining and were rich in calcium and phosphorus according to energy dispersive X-ray analysis. In conclusion, Morinda citrifolia leaf extract promoted osteogenic differentiation and matrix mineralization in human periodontal ligament cells, a clear indication of the therapeutic potential of Morinda citrifolia leaves in bone and periodontal tissue regeneration.

KW - Mineralization

KW - Morinda citrifolia

KW - Osteogenic differentiation

KW - Periodontal ligament cells

UR - https://www.scopus.com/pages/publications/84867861374

U2 - 10.4012/dmj.2012-053

DO - 10.4012/dmj.2012-053

M3 - Article

C2 - 23037852

AN - SCOPUS:84867861374

SN - 0287-4547

VL - 31

SP - 863

EP - 871

JO - Dental Materials Journal

JF - Dental Materials Journal

IS - 5

ER -

Morinda citrifolia leaves enhance osteogenic differentiation and mineralization of human periodontal ligament cells

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this