@inproceedings{aa070694f44947a795c71f3c18c92d17,
title = "Micropropagation of Rhynchostylis gigantea var. rubra through protocorm-like bodies",
abstract = "Rhynchostylis gigantea var. rubra is a variety of purple-red flower orchid. There is small amount of this orchid in natural habitat. Shoot-tip culture was applied for asexual propagation to maintain its good characteristics. Efficient methods for micropropagation through protocorm-like bodies (PLBs) from shoottips using various plant tissue culture media and sucrose concentrations were studied. After two months of culture, the highest survival rate of 92.6\% and the highest PLB formation (7.9 PLBs) were found in a modified Vacin and Went liquid medium with 1\% (w/v) sucrose. Shoot proliferation was effectively induced by using a basal Schenk and Hildebrandt (SH). PLBs cultured in SH media supplemented with 0.1 mg L-1 thidiazuron (TDZ) produced a high number of shoots/PLB but had no root while those cultured in PGR free SH media or SH media supplemented with 0.5 mg L-1 NAA produced lots of shoots with roots.",
keywords = "Asexual propagation, Micropropagation, Protocorm-like bodies, Shoot proliferation, Shoot-tip culture",
author = "S. Prasongsom and K. Thammasiri and N. Chuenboonngarm and W. Noonpakdee",
year = "2014",
month = mar,
day = "13",
doi = "10.17660/ActaHortic.2014.1025.31",
language = "English",
isbn = "9789462610118",
series = "Acta Horticulturae",
publisher = "International Society for Horticultural Science",
pages = "215--222",
booktitle = "International Symposium on Orchids and Ornamental Plants",
}