TY - GEN
T1 - Mesoporous hybrid membrane based label free electrochemical immunoassay
AU - Pamsubsakul, Attasith
AU - Rijiravanich, Patsamon
AU - Surareungchai, Werasak
N1 - Publisher Copyright:
© 2014 IEEE.
PY - 2014/1/20
Y1 - 2014/1/20
N2 - We demonstrated an application of a mesoporous-silica hybrid membrane (MHM) toward label free electrochemical immunoassay. Mesoporous silica oriented parallel in the columnar pores of a commercial anodic alumina membrane was firstly prepared. The hybrid membrane was further functionalized with antibiodies and followed by interacting with pathogenic samples through immunorecognition event The sensing mechanism relies on the pore blocking from bound bacteria. The blockage could be traced by measuring the decrease in differential pulse voltammetric (DPV) current of ferrocenecarboxylic redox indicator. Herein, we are interested in proof of the concept on characterization of the constructed membrane, testing immunoreactivity on the membrane by ELISA and the detection of the target. The results showed the potential advantage of using this composite nanomaterial for enhancing the sensing response in the application of label free immunoassay.
AB - We demonstrated an application of a mesoporous-silica hybrid membrane (MHM) toward label free electrochemical immunoassay. Mesoporous silica oriented parallel in the columnar pores of a commercial anodic alumina membrane was firstly prepared. The hybrid membrane was further functionalized with antibiodies and followed by interacting with pathogenic samples through immunorecognition event The sensing mechanism relies on the pore blocking from bound bacteria. The blockage could be traced by measuring the decrease in differential pulse voltammetric (DPV) current of ferrocenecarboxylic redox indicator. Herein, we are interested in proof of the concept on characterization of the constructed membrane, testing immunoreactivity on the membrane by ELISA and the detection of the target. The results showed the potential advantage of using this composite nanomaterial for enhancing the sensing response in the application of label free immunoassay.
KW - Mesoporous hybrid membrane
KW - Salmonella typhimurium
KW - immunoassay
KW - label-free electrochemical sensor
UR - https://www.scopus.com/pages/publications/84923032879
U2 - 10.1109/BMEiCON.2014.7017442
DO - 10.1109/BMEiCON.2014.7017442
M3 - Conference contribution
AN - SCOPUS:84923032879
T3 - BMEiCON 2014 - 7th Biomedical Engineering International Conference
BT - BMEiCON 2014 - 7th Biomedical Engineering International Conference
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 7th Biomedical Engineering International Conference, BMEiCON 2014
Y2 - 26 November 2014 through 28 November 2014
ER -