Low-energy heavy-ion-beam-induced mutation of novel high-yielding drought-tolerant Thai Jasmine rice

B. Khitka; B. Phanchaisri; A. Sutipatanasomboon; W. Nuangmek; L. D. Yu; J. Techarang

doi:10.1016/j.nimb.2021.02.003

Low-energy heavy-ion-beam-induced mutation of novel high-yielding drought-tolerant Thai Jasmine rice

B. Khitka
, B. Phanchaisri
, A. Sutipatanasomboon
, W. Nuangmek
, L. D. Yu
, J. Techarang

Agricultural Biotechnology Sciences Academic Group

University of Phayao
Chiang Mai University
CHE

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

To respond to the global warming challenge to local agriculture, we applied a low-energy heavy ion beam as novel biotechnology to improve crop drought tolerance. Dehulled seeds of Thai Jasmine rice, Khao Dawk Mali 105 (KDML105), a popular Thai rice variety, were bombarded by a nitrogen ion beam with energy of tens-keV and fluences in an order of 10¹⁶ ions/cm². The resulting mutagenized rice was obtained, selected, and further advanced until phenotypically stable. Mutagenized lines were screened for drought tolerance in the seedling and reproductive stages under an artificially created drought condition in comparison with KDML105 and the drought-tolerant reference variety, CT9993, using both subjective and quantitative parameters. We advanced the mutants up to M8 generation for stabilization and found a mutant line named HyKOS22, which displayed higher tolerance to the drought condition than KDML105 and CT9993 and produced a higher crop yield than KDML105. Genetic changes in HyKOS22 were confirmed by molecular biology analysis.

Original language	English
Pages (from-to)	34-42
Number of pages	9
Journal	Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume	492
DOIs	https://doi.org/10.1016/j.nimb.2021.02.003
Publication status	Published - 1 Apr 2021

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 2 Zero Hunger
SDG 13 Climate Action

Keywords

Drought tolerance
Low-energy heavy ion beam (LEHIB)
Mutation
Rice
Yield

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10.1016/j.nimb.2021.02.003

Cite this

@article{55e6f24a5504488ab2a181e1febc0975,

title = "Low-energy heavy-ion-beam-induced mutation of novel high-yielding drought-tolerant Thai Jasmine rice",

abstract = "To respond to the global warming challenge to local agriculture, we applied a low-energy heavy ion beam as novel biotechnology to improve crop drought tolerance. Dehulled seeds of Thai Jasmine rice, Khao Dawk Mali 105 (KDML105), a popular Thai rice variety, were bombarded by a nitrogen ion beam with energy of tens-keV and fluences in an order of 1016 ions/cm2. The resulting mutagenized rice was obtained, selected, and further advanced until phenotypically stable. Mutagenized lines were screened for drought tolerance in the seedling and reproductive stages under an artificially created drought condition in comparison with KDML105 and the drought-tolerant reference variety, CT9993, using both subjective and quantitative parameters. We advanced the mutants up to M8 generation for stabilization and found a mutant line named HyKOS22, which displayed higher tolerance to the drought condition than KDML105 and CT9993 and produced a higher crop yield than KDML105. Genetic changes in HyKOS22 were confirmed by molecular biology analysis.",

keywords = "Drought tolerance, Low-energy heavy ion beam (LEHIB), Mutation, Rice, Yield",

author = "B. Khitka and B. Phanchaisri and A. Sutipatanasomboon and W. Nuangmek and Yu, \{L. D.\} and J. Techarang",

note = "Publisher Copyright: {\textcopyright} 2021 Elsevier B.V.",

year = "2021",

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day = "1",

doi = "10.1016/j.nimb.2021.02.003",

language = "English",

volume = "492",

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}

Low-energy heavy-ion-beam-induced mutation of novel high-yielding drought-tolerant Thai Jasmine rice. / Khitka, B.; Phanchaisri, B.; Sutipatanasomboon, A. et al.
In: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, Vol. 492, 01.04.2021, p. 34-42.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Low-energy heavy-ion-beam-induced mutation of novel high-yielding drought-tolerant Thai Jasmine rice

AU - Khitka, B.

AU - Phanchaisri, B.

AU - Sutipatanasomboon, A.

AU - Nuangmek, W.

AU - Yu, L. D.

AU - Techarang, J.

PY - 2021/4/1

Y1 - 2021/4/1

N2 - To respond to the global warming challenge to local agriculture, we applied a low-energy heavy ion beam as novel biotechnology to improve crop drought tolerance. Dehulled seeds of Thai Jasmine rice, Khao Dawk Mali 105 (KDML105), a popular Thai rice variety, were bombarded by a nitrogen ion beam with energy of tens-keV and fluences in an order of 1016 ions/cm2. The resulting mutagenized rice was obtained, selected, and further advanced until phenotypically stable. Mutagenized lines were screened for drought tolerance in the seedling and reproductive stages under an artificially created drought condition in comparison with KDML105 and the drought-tolerant reference variety, CT9993, using both subjective and quantitative parameters. We advanced the mutants up to M8 generation for stabilization and found a mutant line named HyKOS22, which displayed higher tolerance to the drought condition than KDML105 and CT9993 and produced a higher crop yield than KDML105. Genetic changes in HyKOS22 were confirmed by molecular biology analysis.

AB - To respond to the global warming challenge to local agriculture, we applied a low-energy heavy ion beam as novel biotechnology to improve crop drought tolerance. Dehulled seeds of Thai Jasmine rice, Khao Dawk Mali 105 (KDML105), a popular Thai rice variety, were bombarded by a nitrogen ion beam with energy of tens-keV and fluences in an order of 1016 ions/cm2. The resulting mutagenized rice was obtained, selected, and further advanced until phenotypically stable. Mutagenized lines were screened for drought tolerance in the seedling and reproductive stages under an artificially created drought condition in comparison with KDML105 and the drought-tolerant reference variety, CT9993, using both subjective and quantitative parameters. We advanced the mutants up to M8 generation for stabilization and found a mutant line named HyKOS22, which displayed higher tolerance to the drought condition than KDML105 and CT9993 and produced a higher crop yield than KDML105. Genetic changes in HyKOS22 were confirmed by molecular biology analysis.

KW - Drought tolerance

KW - Low-energy heavy ion beam (LEHIB)

KW - Mutation

KW - Rice

KW - Yield

UR - https://www.scopus.com/pages/publications/85100883510

U2 - 10.1016/j.nimb.2021.02.003

DO - 10.1016/j.nimb.2021.02.003

M3 - Article

AN - SCOPUS:85100883510

SN - 0168-583X

VL - 492

SP - 34

EP - 42

JO - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms

JF - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms

ER -

Low-energy heavy-ion-beam-induced mutation of novel high-yielding drought-tolerant Thai Jasmine rice

Abstract

UN SDGs

Keywords

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