Lemongrass-Incorporated Tissue Conditioner with Adjustable Inhibitory Effect Against Candida albicans: An In Vitro Study

Purpose: To develop and assess lemongrass-incorporated tissue conditioners (LG-TCs) with a potent and long-lasting inhibitory effect against Candida albicans cultures to control the accumulation of fungi. Materials and Methods: LG essential oil with concentrations of 7.17%, 3.56%, 1.77%, and 0.89% (w/w) or nystatin were mixed with the liquid part of the TC before being added to the powder part to form 486 TC samples of 6-mm diameter × 2-mm thickness (n = 81 samples for each group of LG-/nystatin-incorporated or unmodified TCs). After being immersed in 37°C water for 1, 2, 3, 5, 7, 9, 12, or 14 days, these TC samples were removed, blotted with sterile filter paper, and then exposed to fungal suspension (1 × 105 CFU/mL). The TC samples were evaluated for their capacity to inhibit fungal growth by 99.9%. Results: The anti-Candida effect of the unmodified TCs was reduced significantly after the samples were immersed in water. Interestingly, a long-lasting anti-Candida effect was observed in the TCs incorporated with LG essential oil. After being immersed in water for at least 14 days, the TCs with 1.77% LG oil were still able to inhibit fungal growth substantially. In contrast, a shorter-lasting (5 days) anti-Candida effect was found in the TCs with 0.89% (w/w) LG oil. Additionally, the TCs incorporated with LG oil at concentrations of 3.56% (w/w) or more inhibited the growth of the fungus by 99.99%, and its anti-Candida effect lasted for 14 days. Conclusion: LG-TCs showed an impressive and long-lasting inhibitory effect against C.