Label-free, real-time glycosyltransferase assay based on a fluorescent artificial chemosensor

Jirarut Wongkongkatep; Yoshifumi Miyahara; Akio Ojida; Itaru Hamachi

doi:10.1002/anie.200503107

Label-free, real-time glycosyltransferase assay based on a fluorescent artificial chemosensor

Jirarut Wongkongkatep
, Yoshifumi Miyahara
, Akio Ojida
, Itaru Hamachi

Kyoto University

Research output: Contribution to journal › Article › peer-review

76 Citations (Scopus)

Abstract

Reality check: The fluorescent probe 1 is applied to a real-time glycosyltransferase assay. The fluorescence of 1 increases with the progress of the glycosyl transfer reaction. The high sensitivity and reliable output of the method under multisubstrate enzymatic conditions make it suitable for the high-throughput screening of glycosyltransferase inhibitors. (Chemical Equation Presented)

Original language	English
Pages (from-to)	665-668
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	45
Issue number	4
DOIs	https://doi.org/10.1002/anie.200503107
Publication status	Published - 16 Jan 2006
Externally published	Yes

Keywords

Fluorescent probes
Glycosylation
Sensors
Supramolecular chemistry
Transferases

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10.1002/anie.200503107

Cite this

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abstract = "Reality check: The fluorescent probe 1 is applied to a real-time glycosyltransferase assay. The fluorescence of 1 increases with the progress of the glycosyl transfer reaction. The high sensitivity and reliable output of the method under multisubstrate enzymatic conditions make it suitable for the high-throughput screening of glycosyltransferase inhibitors. (Chemical Equation Presented)",

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AU - Wongkongkatep, Jirarut

AU - Miyahara, Yoshifumi

AU - Ojida, Akio

AU - Hamachi, Itaru

PY - 2006/1/16

Y1 - 2006/1/16

N2 - Reality check: The fluorescent probe 1 is applied to a real-time glycosyltransferase assay. The fluorescence of 1 increases with the progress of the glycosyl transfer reaction. The high sensitivity and reliable output of the method under multisubstrate enzymatic conditions make it suitable for the high-throughput screening of glycosyltransferase inhibitors. (Chemical Equation Presented)

AB - Reality check: The fluorescent probe 1 is applied to a real-time glycosyltransferase assay. The fluorescence of 1 increases with the progress of the glycosyl transfer reaction. The high sensitivity and reliable output of the method under multisubstrate enzymatic conditions make it suitable for the high-throughput screening of glycosyltransferase inhibitors. (Chemical Equation Presented)

KW - Fluorescent probes

KW - Glycosylation

KW - Sensors

KW - Supramolecular chemistry

KW - Transferases

UR - https://www.scopus.com/pages/publications/31044442787

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Label-free, real-time glycosyltransferase assay based on a fluorescent artificial chemosensor

Abstract

Keywords

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