International laboratory comparison of influenza microneutralization assays for A(H1N1)pdm09, A(H3N2), and A(H5N1) influenza viruses by CONSISE

the Consise Laboratory Working Group Participants

doi:10.1128/CVI.00278-15

International laboratory comparison of influenza microneutralization assays for A(H1N1)pdm09, A(H3N2), and A(H5N1) influenza viruses by CONSISE

the Consise Laboratory Working Group Participants

Center for Research Innovation and Biomedical Informatics

Peter Doherty Institute for Infection and Immunity
National Institute for Biological Standards and Control
TB Laboratory Research, CDC
The University of Hong Kong Li Ka Shing Faculty of Medicine
Imperial College London
Norwegian Institute of Public Health
World Health Organization
Institut Pasteur, Paris
Siriraj Hospital
Italian National Institute of Health
NIH
National Institute of Virology India
U.S. Naval Health Research Center
Naval Medical Research Center
Paul-Ehrlich Institute
Università di Siena
Chinese National Influenza Center
Victorian Infectious Diseases Reference Laboratory

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

The microneutralization assay is commonly used to detect antibodies to influenza virus, and multiple protocols are used worldwide. These protocols differ in the incubation time of the assay as well as in the order of specific steps, and even within protocols there are often further adjustments in individual laboratories. The impact these protocol variations have on influenza serology data is unclear. Thus, a laboratory comparison of the 2-day enzyme-linked immunosorbent assay (ELISA) and 3-day hemagglutination (HA) microneutralization (MN) protocols, using A(H1N1)pdm09, A(H3N2), and A(H5N1) viruses, was performed by the CONSISE Laboratory Working Group. Individual laboratories performed both assay protocols, on multiple occasions, using different serum panels. Thirteen laboratories from around the world participated. Within each laboratory, serum sample titers for the different assay protocols were compared between assays to determine the sensitivity of each assay and were compared between replicates to assess the reproducibility of each protocol for each laboratory. There was good correlation of the results obtained using the two assay protocols in most laboratories, indicating that these assays may be interchangeable for detecting antibodies to the influenza A viruses included in this study. Importantly, participating laboratories have aligned their methodologies to the CONSISE consensus 2-day ELISA and 3-day HAMNassay protocols to enable better correlation of these assays in the future.

Original language	English
Pages (from-to)	957-964
Number of pages	8
Journal	Clinical and Vaccine Immunology
Volume	22
Issue number	8
DOIs	https://doi.org/10.1128/CVI.00278-15
Publication status	Published - 1 Aug 2015

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10.1128/CVI.00278-15

Cite this

@article{5e551c90d6724a25b99977996e21e02d,

title = "International laboratory comparison of influenza microneutralization assays for A(H1N1)pdm09, A(H3N2), and A(H5N1) influenza viruses by CONSISE",

abstract = "The microneutralization assay is commonly used to detect antibodies to influenza virus, and multiple protocols are used worldwide. These protocols differ in the incubation time of the assay as well as in the order of specific steps, and even within protocols there are often further adjustments in individual laboratories. The impact these protocol variations have on influenza serology data is unclear. Thus, a laboratory comparison of the 2-day enzyme-linked immunosorbent assay (ELISA) and 3-day hemagglutination (HA) microneutralization (MN) protocols, using A(H1N1)pdm09, A(H3N2), and A(H5N1) viruses, was performed by the CONSISE Laboratory Working Group. Individual laboratories performed both assay protocols, on multiple occasions, using different serum panels. Thirteen laboratories from around the world participated. Within each laboratory, serum sample titers for the different assay protocols were compared between assays to determine the sensitivity of each assay and were compared between replicates to assess the reproducibility of each protocol for each laboratory. There was good correlation of the results obtained using the two assay protocols in most laboratories, indicating that these assays may be interchangeable for detecting antibodies to the influenza A viruses included in this study. Importantly, participating laboratories have aligned their methodologies to the CONSISE consensus 2-day ELISA and 3-day HAMNassay protocols to enable better correlation of these assays in the future.",

author = "\{the Consise Laboratory Working Group Participants\} and Laurie, \{Karen L.\} and Engelhardt, \{Othmar G.\} and John Wood and Alan Heath and Katz, \{Jacqueline M.\} and Malik Peiris and Katja Hoschler and Olav Hungnes and Wenqing Zhang and \{Van Kerkhove\}, \{Maria D.\} and Jaccqueline Katz and Xiuhua Lu and Min Levine and Vic Veguilla and Feng Liu and Yaohui Bai and Pilaipan Puthavathana and Hatairat Lerdsamran and Phisanu Pooruk and Knnika Nateerom and Castrucci, \{Maria Rita\} and Isabella Donatelli and Marzia Facchini and Noriko Kishida and Masato Tashiro and Takato Odagiri and Pawar, \{Shailesh D.\} and Kode, \{Sadhana S.\} and Anthony Hawksworth and Ryan Ortiguerra and Gary Brice and Nicholas Martin and Tad Kochel and Jose Sanchez and Michael Cooper and James Cummings and Ralf Wagner and Constanze Goepfert and Nina Alex and Joanna Hammann and Britta Neumann and Mahendra Perera and Emanuele Montomoli and Guilia Lapini and Sara Sbragi and Tian Bai and Zaijiang Yu and Jianfang Zhou and Louise Carolan and Karen Laurie",

year = "2015",

month = aug,

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doi = "10.1128/CVI.00278-15",

language = "English",

volume = "22",

pages = "957--964",

journal = "Clinical and Vaccine Immunology",

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AU - the Consise Laboratory Working Group Participants

AU - Laurie, Karen L.

AU - Engelhardt, Othmar G.

AU - Wood, John

AU - Heath, Alan

AU - Katz, Jacqueline M.

AU - Peiris, Malik

AU - Hoschler, Katja

AU - Hungnes, Olav

AU - Zhang, Wenqing

AU - Van Kerkhove, Maria D.

AU - Katz, Jaccqueline

AU - Lu, Xiuhua

AU - Levine, Min

AU - Veguilla, Vic

AU - Liu, Feng

AU - Bai, Yaohui

AU - Puthavathana, Pilaipan

AU - Lerdsamran, Hatairat

AU - Pooruk, Phisanu

AU - Nateerom, Knnika

AU - Castrucci, Maria Rita

AU - Donatelli, Isabella

AU - Facchini, Marzia

AU - Kishida, Noriko

AU - Tashiro, Masato

AU - Odagiri, Takato

AU - Pawar, Shailesh D.

AU - Kode, Sadhana S.

AU - Hawksworth, Anthony

AU - Ortiguerra, Ryan

AU - Brice, Gary

AU - Martin, Nicholas

AU - Kochel, Tad

AU - Sanchez, Jose

AU - Cooper, Michael

AU - Cummings, James

AU - Wagner, Ralf

AU - Goepfert, Constanze

AU - Alex, Nina

AU - Hammann, Joanna

AU - Neumann, Britta

AU - Perera, Mahendra

AU - Montomoli, Emanuele

AU - Lapini, Guilia

AU - Sbragi, Sara

AU - Bai, Tian

AU - Yu, Zaijiang

AU - Zhou, Jianfang

AU - Carolan, Louise

AU - Laurie, Karen

PY - 2015/8/1

Y1 - 2015/8/1

N2 - The microneutralization assay is commonly used to detect antibodies to influenza virus, and multiple protocols are used worldwide. These protocols differ in the incubation time of the assay as well as in the order of specific steps, and even within protocols there are often further adjustments in individual laboratories. The impact these protocol variations have on influenza serology data is unclear. Thus, a laboratory comparison of the 2-day enzyme-linked immunosorbent assay (ELISA) and 3-day hemagglutination (HA) microneutralization (MN) protocols, using A(H1N1)pdm09, A(H3N2), and A(H5N1) viruses, was performed by the CONSISE Laboratory Working Group. Individual laboratories performed both assay protocols, on multiple occasions, using different serum panels. Thirteen laboratories from around the world participated. Within each laboratory, serum sample titers for the different assay protocols were compared between assays to determine the sensitivity of each assay and were compared between replicates to assess the reproducibility of each protocol for each laboratory. There was good correlation of the results obtained using the two assay protocols in most laboratories, indicating that these assays may be interchangeable for detecting antibodies to the influenza A viruses included in this study. Importantly, participating laboratories have aligned their methodologies to the CONSISE consensus 2-day ELISA and 3-day HAMNassay protocols to enable better correlation of these assays in the future.

AB - The microneutralization assay is commonly used to detect antibodies to influenza virus, and multiple protocols are used worldwide. These protocols differ in the incubation time of the assay as well as in the order of specific steps, and even within protocols there are often further adjustments in individual laboratories. The impact these protocol variations have on influenza serology data is unclear. Thus, a laboratory comparison of the 2-day enzyme-linked immunosorbent assay (ELISA) and 3-day hemagglutination (HA) microneutralization (MN) protocols, using A(H1N1)pdm09, A(H3N2), and A(H5N1) viruses, was performed by the CONSISE Laboratory Working Group. Individual laboratories performed both assay protocols, on multiple occasions, using different serum panels. Thirteen laboratories from around the world participated. Within each laboratory, serum sample titers for the different assay protocols were compared between assays to determine the sensitivity of each assay and were compared between replicates to assess the reproducibility of each protocol for each laboratory. There was good correlation of the results obtained using the two assay protocols in most laboratories, indicating that these assays may be interchangeable for detecting antibodies to the influenza A viruses included in this study. Importantly, participating laboratories have aligned their methodologies to the CONSISE consensus 2-day ELISA and 3-day HAMNassay protocols to enable better correlation of these assays in the future.

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U2 - 10.1128/CVI.00278-15

DO - 10.1128/CVI.00278-15

M3 - Article

C2 - 26108286

AN - SCOPUS:84939480223

SN - 1556-6811

VL - 22

SP - 957

EP - 964

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

IS - 8

ER -

International laboratory comparison of influenza microneutralization assays for A(H1N1)pdm09, A(H3N2), and A(H5N1) influenza viruses by CONSISE

Abstract

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