Influence of conjugating folic acid and methotrexate on physicochemical properties of P(CL)-mPEG nanoparticles

O. Issarachot; J. Suksiriworapong; K. Sripha; R. Yumoto; M. Takano; V. B. Junyaprasert

Influence of conjugating folic acid and methotrexate on physicochemical properties of P(CL)-mPEG nanoparticles

O. Issarachot
, J. Suksiriworapong
, K. Sripha
, R. Yumoto
, M. Takano
, V. B. Junyaprasert

Mahidol University
Hiroshima University Graduate School of Biomedical and Health Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

The objective of this study was to investigate an effect of conjugating folic acid (FOL) as a targeting ligand and conjugating methotrexate (MTX) as an anticancer drug on the formation of nanoparticles (NPs). MTX-conjugated nanoparticles were prepared using 10, 20 and 30 % MTX conjugated poly(e-caprolactone)-co-methoxy poly(ethylene glycol) (P(MTXCLCL)-mPEG) copolymers by dialysis method. Additionally, the targeted nanoparticles were obtained by the physical adsorption of FOL-conjugated (P(FOLCL)-mPEG) copolymer on the surface of P(MTXCLCL)-mPEG nanoparticles. The particle size of P(MTXCLCL)-mPEG nanoparticles increased with an amount of conjugating MTX. After the adsorption of P(FOLCL)-mPEG, the nanoparticles became larger and the zeta potential turned to more negative by approximately two times. All nanoparticles were spherical in shape and uniform size distribution. The % yield of nanoparticles ranged between 52-58%. The release profile showed higher release of MTX in acidic pH medium.

Original language	English
Title of host publication	NSTI
Subtitle of host publication	Biotech, Biomaterials and Biomedical - TechConnect Briefs 2015
Editors	Matthew Laudon, Bart Romanowicz
Publisher	Taylor and Francis Inc.
Pages	94-97
Number of pages	4
ISBN (Electronic)	9781498747295
Publication status	Published - 2015
Event	10th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 18th Annual Nanotech Conference and Expo, and the 2015 National SBIR/STTR Conference - Washington, United States Duration: 14 Jun 2015 → 17 Jun 2015

Publication series

Name	NSTI: Advanced Materials - TechConnect Briefs 2015
Volume	3

Conference

Conference	10th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 18th Annual Nanotech Conference and Expo, and the 2015 National SBIR/STTR Conference
Country/Territory	United States
City	Washington
Period	14/06/15 → 17/06/15

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Folic acid
Methotrexate
Poly(e -caprolactone)-co-methoxy poly(ethylene glycol)
Targeted nanoparticles

Cite this

Issarachot, O., Suksiriworapong, J., Sripha, K., Yumoto, R., Takano, M., & Junyaprasert, V. B. (2015). Influence of conjugating folic acid and methotrexate on physicochemical properties of P(CL)-mPEG nanoparticles. In M. Laudon, & B. Romanowicz (Eds.), NSTI: Biotech, Biomaterials and Biomedical - TechConnect Briefs 2015 (pp. 94-97). (NSTI: Advanced Materials - TechConnect Briefs 2015; Vol. 3). Taylor and Francis Inc..

Issarachot, O. ; Suksiriworapong, J. ; Sripha, K. et al. / Influence of conjugating folic acid and methotrexate on physicochemical properties of P(CL)-mPEG nanoparticles. NSTI: Biotech, Biomaterials and Biomedical - TechConnect Briefs 2015. editor / Matthew Laudon ; Bart Romanowicz. Taylor and Francis Inc., 2015. pp. 94-97 (NSTI: Advanced Materials - TechConnect Briefs 2015).

@inproceedings{1b044044d6854cd0987cebdc2e8a71de,

title = "Influence of conjugating folic acid and methotrexate on physicochemical properties of P(CL)-mPEG nanoparticles",

abstract = "The objective of this study was to investigate an effect of conjugating folic acid (FOL) as a targeting ligand and conjugating methotrexate (MTX) as an anticancer drug on the formation of nanoparticles (NPs). MTX-conjugated nanoparticles were prepared using 10, 20 and 30 \% MTX conjugated poly(e-caprolactone)-co-methoxy poly(ethylene glycol) (P(MTXCLCL)-mPEG) copolymers by dialysis method. Additionally, the targeted nanoparticles were obtained by the physical adsorption of FOL-conjugated (P(FOLCL)-mPEG) copolymer on the surface of P(MTXCLCL)-mPEG nanoparticles. The particle size of P(MTXCLCL)-mPEG nanoparticles increased with an amount of conjugating MTX. After the adsorption of P(FOLCL)-mPEG, the nanoparticles became larger and the zeta potential turned to more negative by approximately two times. All nanoparticles were spherical in shape and uniform size distribution. The \% yield of nanoparticles ranged between 52-58\%. The release profile showed higher release of MTX in acidic pH medium.",

keywords = "Folic acid, Methotrexate, Poly(e -caprolactone)-co-methoxy poly(ethylene glycol), Targeted nanoparticles",

author = "O. Issarachot and J. Suksiriworapong and K. Sripha and R. Yumoto and M. Takano and Junyaprasert, \{V. B.\}",

note = "Publisher Copyright: Copyright 2015 by TechConnect. All rights reserved.; 10th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 18th Annual Nanotech Conference and Expo, and the 2015 National SBIR/STTR Conference ; Conference date: 14-06-2015 Through 17-06-2015",

year = "2015",

language = "English",

series = "NSTI: Advanced Materials - TechConnect Briefs 2015",

publisher = "Taylor and Francis Inc.",

pages = "94--97",

editor = "Matthew Laudon and Bart Romanowicz",

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}

Issarachot, O, Suksiriworapong, J , Sripha, K, Yumoto, R, Takano, M & Junyaprasert, VB 2015, Influence of conjugating folic acid and methotrexate on physicochemical properties of P(CL)-mPEG nanoparticles. in M Laudon & B Romanowicz (eds), NSTI: Biotech, Biomaterials and Biomedical - TechConnect Briefs 2015. NSTI: Advanced Materials - TechConnect Briefs 2015, vol. 3, Taylor and Francis Inc., pp. 94-97, 10th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 18th Annual Nanotech Conference and Expo, and the 2015 National SBIR/STTR Conference, Washington, United States, 14/06/15.

Influence of conjugating folic acid and methotrexate on physicochemical properties of P(CL)-mPEG nanoparticles. / Issarachot, O.; Suksiriworapong, J.; Sripha, K. et al.
NSTI: Biotech, Biomaterials and Biomedical - TechConnect Briefs 2015. ed. / Matthew Laudon; Bart Romanowicz. Taylor and Francis Inc., 2015. p. 94-97 (NSTI: Advanced Materials - TechConnect Briefs 2015; Vol. 3).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Influence of conjugating folic acid and methotrexate on physicochemical properties of P(CL)-mPEG nanoparticles

AU - Issarachot, O.

AU - Suksiriworapong, J.

AU - Sripha, K.

AU - Yumoto, R.

AU - Takano, M.

AU - Junyaprasert, V. B.

PY - 2015

Y1 - 2015

N2 - The objective of this study was to investigate an effect of conjugating folic acid (FOL) as a targeting ligand and conjugating methotrexate (MTX) as an anticancer drug on the formation of nanoparticles (NPs). MTX-conjugated nanoparticles were prepared using 10, 20 and 30 % MTX conjugated poly(e-caprolactone)-co-methoxy poly(ethylene glycol) (P(MTXCLCL)-mPEG) copolymers by dialysis method. Additionally, the targeted nanoparticles were obtained by the physical adsorption of FOL-conjugated (P(FOLCL)-mPEG) copolymer on the surface of P(MTXCLCL)-mPEG nanoparticles. The particle size of P(MTXCLCL)-mPEG nanoparticles increased with an amount of conjugating MTX. After the adsorption of P(FOLCL)-mPEG, the nanoparticles became larger and the zeta potential turned to more negative by approximately two times. All nanoparticles were spherical in shape and uniform size distribution. The % yield of nanoparticles ranged between 52-58%. The release profile showed higher release of MTX in acidic pH medium.

AB - The objective of this study was to investigate an effect of conjugating folic acid (FOL) as a targeting ligand and conjugating methotrexate (MTX) as an anticancer drug on the formation of nanoparticles (NPs). MTX-conjugated nanoparticles were prepared using 10, 20 and 30 % MTX conjugated poly(e-caprolactone)-co-methoxy poly(ethylene glycol) (P(MTXCLCL)-mPEG) copolymers by dialysis method. Additionally, the targeted nanoparticles were obtained by the physical adsorption of FOL-conjugated (P(FOLCL)-mPEG) copolymer on the surface of P(MTXCLCL)-mPEG nanoparticles. The particle size of P(MTXCLCL)-mPEG nanoparticles increased with an amount of conjugating MTX. After the adsorption of P(FOLCL)-mPEG, the nanoparticles became larger and the zeta potential turned to more negative by approximately two times. All nanoparticles were spherical in shape and uniform size distribution. The % yield of nanoparticles ranged between 52-58%. The release profile showed higher release of MTX in acidic pH medium.

KW - Folic acid

KW - Methotrexate

KW - Poly(e -caprolactone)-co-methoxy poly(ethylene glycol)

KW - Targeted nanoparticles

UR - https://www.scopus.com/pages/publications/84983389473

M3 - Conference contribution

AN - SCOPUS:84983389473

T3 - NSTI: Advanced Materials - TechConnect Briefs 2015

SP - 94

EP - 97

BT - NSTI

A2 - Laudon, Matthew

A2 - Romanowicz, Bart

PB - Taylor and Francis Inc.

T2 - 10th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 18th Annual Nanotech Conference and Expo, and the 2015 National SBIR/STTR Conference

Y2 - 14 June 2015 through 17 June 2015

ER -

Issarachot O, Suksiriworapong J , Sripha K, Yumoto R, Takano M, Junyaprasert VB. Influence of conjugating folic acid and methotrexate on physicochemical properties of P(CL)-mPEG nanoparticles. In Laudon M, Romanowicz B, editors, NSTI: Biotech, Biomaterials and Biomedical - TechConnect Briefs 2015. Taylor and Francis Inc. 2015. p. 94-97. (NSTI: Advanced Materials - TechConnect Briefs 2015).

Influence of conjugating folic acid and methotrexate on physicochemical properties of P(CL)-mPEG nanoparticles

Abstract

Publication series

Conference

UN SDGs

Keywords

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