Imaging single nanoparticle interactions with human lung cells using fast ion conductance microscopy

Pavel Novak; Andrew Shevchuk; Pakatip Ruenraroengsak; Michele Miragoli; Andrew J. Thorley; David Klenerman; Max J. Lab; Teresa D. Tetley; Julia Gorelik; Yuri E. Korchev

doi:10.1021/nl404068p

Imaging single nanoparticle interactions with human lung cells using fast ion conductance microscopy

Pavel Novak
, Andrew Shevchuk
, Pakatip Ruenraroengsak
, Michele Miragoli
, Andrew J. Thorley
, David Klenerman
, Max J. Lab
, Teresa D. Tetley
, Julia Gorelik
, Yuri E. Korchev

Queen Mary University of London
Imperial College London
Department of National Heart and Lung Institute
University of Parma
Personalized Medicine Asthma and Allergy Clinic-Humanitas University & Research Hospital
University of Cambridge

Research output: Contribution to journal › Article › peer-review

86 Citations (Scopus)

Abstract

Experimental data on dynamic interactions between individual nanoparticles and membrane processes at nanoscale, essential for biomedical applications of nanoparticles, remain scarce due to limitations of imaging techniques. We were able to follow single 200 nm carboxyl-modified particles interacting with identified membrane structures at the rate of 15 s/frame using a scanning ion conductance microscope modified for simultaneous high-speed topographical and fluorescence imaging. The imaging approach demonstrated here opens a new window into the complexity of nanoparticle-cell interactions.

Original language	English
Pages (from-to)	1202-1207
Number of pages	6
Journal	Nano Letters
Volume	14
Issue number	3
DOIs	https://doi.org/10.1021/nl404068p
Publication status	Published - 12 Mar 2014
Externally published	Yes

Keywords

Nanoparticles
actin
alveolar epithelial cells
clathrin
scanning ion conductance microscopy

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10.1021/nl404068p

Cite this

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title = "Imaging single nanoparticle interactions with human lung cells using fast ion conductance microscopy",

abstract = "Experimental data on dynamic interactions between individual nanoparticles and membrane processes at nanoscale, essential for biomedical applications of nanoparticles, remain scarce due to limitations of imaging techniques. We were able to follow single 200 nm carboxyl-modified particles interacting with identified membrane structures at the rate of 15 s/frame using a scanning ion conductance microscope modified for simultaneous high-speed topographical and fluorescence imaging. The imaging approach demonstrated here opens a new window into the complexity of nanoparticle-cell interactions.",

keywords = "Nanoparticles, actin, alveolar epithelial cells, clathrin, scanning ion conductance microscopy",

author = "Pavel Novak and Andrew Shevchuk and Pakatip Ruenraroengsak and Michele Miragoli and Thorley, \{Andrew J.\} and David Klenerman and Lab, \{Max J.\} and Tetley, \{Teresa D.\} and Julia Gorelik and Korchev, \{Yuri E.\}",

year = "2014",

month = mar,

day = "12",

doi = "10.1021/nl404068p",

language = "English",

volume = "14",

pages = "1202--1207",

journal = "Nano Letters",

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T1 - Imaging single nanoparticle interactions with human lung cells using fast ion conductance microscopy

AU - Novak, Pavel

AU - Shevchuk, Andrew

AU - Ruenraroengsak, Pakatip

AU - Miragoli, Michele

AU - Thorley, Andrew J.

AU - Klenerman, David

AU - Lab, Max J.

AU - Tetley, Teresa D.

AU - Gorelik, Julia

AU - Korchev, Yuri E.

PY - 2014/3/12

Y1 - 2014/3/12

N2 - Experimental data on dynamic interactions between individual nanoparticles and membrane processes at nanoscale, essential for biomedical applications of nanoparticles, remain scarce due to limitations of imaging techniques. We were able to follow single 200 nm carboxyl-modified particles interacting with identified membrane structures at the rate of 15 s/frame using a scanning ion conductance microscope modified for simultaneous high-speed topographical and fluorescence imaging. The imaging approach demonstrated here opens a new window into the complexity of nanoparticle-cell interactions.

AB - Experimental data on dynamic interactions between individual nanoparticles and membrane processes at nanoscale, essential for biomedical applications of nanoparticles, remain scarce due to limitations of imaging techniques. We were able to follow single 200 nm carboxyl-modified particles interacting with identified membrane structures at the rate of 15 s/frame using a scanning ion conductance microscope modified for simultaneous high-speed topographical and fluorescence imaging. The imaging approach demonstrated here opens a new window into the complexity of nanoparticle-cell interactions.

KW - Nanoparticles

KW - actin

KW - alveolar epithelial cells

KW - clathrin

KW - scanning ion conductance microscopy

UR - https://www.scopus.com/pages/publications/84896333372

U2 - 10.1021/nl404068p

DO - 10.1021/nl404068p

M3 - Article

C2 - 24555574

AN - SCOPUS:84896333372

SN - 1530-6984

VL - 14

SP - 1202

EP - 1207

JO - Nano Letters

JF - Nano Letters

IS - 3

ER -

Imaging single nanoparticle interactions with human lung cells using fast ion conductance microscopy

Abstract

Keywords

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