Identification of multispecific organic anion transporter 2 expressed predominantly in the liver

Takashi Sekine; Seok Ho Cha; Minoru Tsuda; Nopporn Apiwattanakul; Noriko Nakajima; Yoshikatsu Kanai; Hitoshi Endou

doi:10.1016/S0014-5793(98)00585-7

Identification of multispecific organic anion transporter 2 expressed predominantly in the liver

Takashi Sekine
, Seok Ho Cha
, Minoru Tsuda
, Nopporn Apiwattanakul
, Noriko Nakajima
, Yoshikatsu Kanai
, Hitoshi Endou

Kyorin University School of Medicine
College of Medicine of the Catholic University of Korea

Research output: Contribution to journal › Article › peer-review

320 Citations (Scopus)

Abstract

In the present study, we demonstrate that NLT (novel liver-specific transport protein) is a multispecific organic anion transporter of the liver. The amino acid sequence of NLT shows 42% identity to that of the renal multispecific organic anion transporter, OAT1. When expressed in Xenopus laevis oocytes, NLT mediated uptake of organic anions, such as salicylate, acetylsalicylate, PGE₂, dicarboxylates and p-aminohippurate. [¹⁴C]Salicylate uptake via NLT was saturable (K(m) = 88.8 ± 23.4 μM) and sodium-independent. Expression of the mRNA of NLT was detected in the liver and kidney (liver >> kidney). We propose that NLT be renamed OAT2.

Original language	English
Pages (from-to)	179-182
Number of pages	4
Journal	FEBS Letters
Volume	429
Issue number	2
DOIs	https://doi.org/10.1016/S0014-5793(98)00585-7
Publication status	Published - 12 Jun 1998
Externally published	Yes

Keywords

Liver
Multispecific transporter
Organic anion
Pharmacokinetics

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10.1016/S0014-5793(98)00585-7

Cite this

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title = "Identification of multispecific organic anion transporter 2 expressed predominantly in the liver",

abstract = "In the present study, we demonstrate that NLT (novel liver-specific transport protein) is a multispecific organic anion transporter of the liver. The amino acid sequence of NLT shows 42\% identity to that of the renal multispecific organic anion transporter, OAT1. When expressed in Xenopus laevis oocytes, NLT mediated uptake of organic anions, such as salicylate, acetylsalicylate, PGE2, dicarboxylates and p-aminohippurate. [14C]Salicylate uptake via NLT was saturable (K(m) = 88.8 ± 23.4 μM) and sodium-independent. Expression of the mRNA of NLT was detected in the liver and kidney (liver >> kidney). We propose that NLT be renamed OAT2.",

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author = "Takashi Sekine and Cha, \{Seok Ho\} and Minoru Tsuda and Nopporn Apiwattanakul and Noriko Nakajima and Yoshikatsu Kanai and Hitoshi Endou",

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T1 - Identification of multispecific organic anion transporter 2 expressed predominantly in the liver

AU - Sekine, Takashi

AU - Cha, Seok Ho

AU - Tsuda, Minoru

AU - Apiwattanakul, Nopporn

AU - Nakajima, Noriko

AU - Kanai, Yoshikatsu

AU - Endou, Hitoshi

PY - 1998/6/12

Y1 - 1998/6/12

N2 - In the present study, we demonstrate that NLT (novel liver-specific transport protein) is a multispecific organic anion transporter of the liver. The amino acid sequence of NLT shows 42% identity to that of the renal multispecific organic anion transporter, OAT1. When expressed in Xenopus laevis oocytes, NLT mediated uptake of organic anions, such as salicylate, acetylsalicylate, PGE2, dicarboxylates and p-aminohippurate. [14C]Salicylate uptake via NLT was saturable (K(m) = 88.8 ± 23.4 μM) and sodium-independent. Expression of the mRNA of NLT was detected in the liver and kidney (liver >> kidney). We propose that NLT be renamed OAT2.

AB - In the present study, we demonstrate that NLT (novel liver-specific transport protein) is a multispecific organic anion transporter of the liver. The amino acid sequence of NLT shows 42% identity to that of the renal multispecific organic anion transporter, OAT1. When expressed in Xenopus laevis oocytes, NLT mediated uptake of organic anions, such as salicylate, acetylsalicylate, PGE2, dicarboxylates and p-aminohippurate. [14C]Salicylate uptake via NLT was saturable (K(m) = 88.8 ± 23.4 μM) and sodium-independent. Expression of the mRNA of NLT was detected in the liver and kidney (liver >> kidney). We propose that NLT be renamed OAT2.

KW - Liver

KW - Multispecific transporter

KW - Organic anion

KW - Pharmacokinetics

UR - https://www.scopus.com/pages/publications/17144449057

U2 - 10.1016/S0014-5793(98)00585-7

DO - 10.1016/S0014-5793(98)00585-7

M3 - Article

C2 - 9650585

AN - SCOPUS:17144449057

SN - 0014-5793

VL - 429

SP - 179

EP - 182

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Identification of multispecific organic anion transporter 2 expressed predominantly in the liver

Abstract

Keywords

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