Abstract
In this study, we utilized a technique called ligation-mediated rolling circle amplification (L-RCA) that combined padlock probe ligation and rolling circle amplification techniques to determine human sex. Amelogenin X and Y specific padlock probes were designed based on polymorphisms and the 6-bp indel of the amelogenin X and Y alleles. Lengths of DNA target for padlock probe detection were 40 and 33 bases of amelogenin X and Y alleles, respectively. Analysis of female DNA samples produced one L-RCA product from the AmelX padlock probe, whereas 2 different types of products were amplified from male DNA samples, each generated by AmelX and AmelY padlock probes. Utilization of padlock probes via L-RCA technique would provide an alternative tool for human sex determination in highly degraded DNA analysis in the future.
| Original language | English |
|---|---|
| Pages (from-to) | e506-e507 |
| Journal | Forensic Science International: Genetics Supplement Series |
| Volume | 3 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Dec 2011 |
Keywords
- Amelogenin gene
- Ligation-mediated rolling circle amplification
- Padlock probe
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