Abstract
The feasibility of trace metal speciation in proteins directly using flow field-flow fractionation-inductively coupled plasma mass spectrometry (FIFFF-ICP-MS) was investigated. The effect on the separation of the FIFFF system cross flow rate was demonstrated for a carbonic anhydrase and alcohol dehydrogenase mixture. Preliminary studies with FIFFF-ICP-MS were applied to several protein standards including metallothionein, carbonic anhydrase, ceruloplasmin, alcohol dehydrogenase, and thyroglobulin. Cadmium was detected in metallothionein; copper and zinc in carbonic anhydrase, alcohol dehydrogenase, and ceruloplasmin, and iodide in thyroglobulin.
| Original language | English |
|---|---|
| Pages (from-to) | 1527-1531 |
| Number of pages | 5 |
| Journal | Journal of Analytical Atomic Spectrometry |
| Volume | 14 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 1999 |
| Externally published | Yes |
| Event | Proceedings of the 1999 European Winter Conference on Plasma Spectrochemistry - Pau Duration: 10 Jan 1999 → 15 Jan 1999 |
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