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Fabrication of novel shark collagen-pectin scaffolds for tissue engineering

  • Yaowaporn Sangsen
  • , Soottawat Benjakul
  • , Kwunchit Oungbho
  • Prince of Songkla University

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

10 Citations (Scopus)

Abstract

Collagen-pectin scaffolds comprised of collagen derived from the skins of brown banded bamboo sharks (Chiloscyllium punctatum) and citrus pectin were fabricated for tissue engineering. Crosslinking of the scaffolds was performed by pregelation of pectin using calcium sulfate and chemical crosslinking of collagen using carbodiimide. The surface morphology of the scaffolds as determined by scanning electron microscopy (SEM) showed a porous structure with an average pore size of 134.53 ± 52.44 μm with interconnecting pores. The mechanical properties of the scaffolds were a hardness of 0.591±0.135 N, and a springiness of 0.958±0.022. Compatibility of the scaffolds was confirmed using an in vitro cytotoxicity test on C2C12 myoblast cells. It was concluded that these collagen-pectin scaffolds from a novel source of collagen could be suitable for applications in tissue engineering.

Original languageEnglish
Title of host publicationBMEiCON-2011 - 4th Biomedical Engineering International Conference
Pages273-278
Number of pages6
DOIs
Publication statusPublished - 2011
Externally publishedYes
Event4th Biomedical Engineering International Conference, BMEiCON-2011 - Chiang Mai, Thailand
Duration: 29 Jan 201231 Jan 2012

Publication series

NameBMEiCON-2011 - 4th Biomedical Engineering International Conference

Conference

Conference4th Biomedical Engineering International Conference, BMEiCON-2011
Country/TerritoryThailand
CityChiang Mai
Period29/01/1231/01/12

Keywords

  • marine collagen
  • pectin
  • scaffold
  • tissue engineering

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