Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts

Sukanya Chumchuen; Chonthicha Satirapod; Amornrat Tangprasittipap; Suradej Hongeng

doi:10.1016/j.scr.2022.102675

Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts

Faculty of Medicine Ramathibodi Hospital, Mahidol University

Research output: Contribution to journal › Article › peer-review

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Abstract

MUi028-A human induced pluripotent stem cell (hiPSC) line was generated from normal fetal skin fibroblasts using a non-integrative reprogramming method. Reprogramming factors OCT4, SOX2, KLF4, L-MYC, and LIN28, and TP53 shRNA in three episomal vectors were delivered by electroporation. The MUi028-A cell line had embryonic stem cell characteristics with consistent expression of specific pluripotency markers and the capability of in vitro differentiation into the three germ layers. This iPSC line can be used as a control to study disease mechanisms.

Original language	English
Article number	102675
Journal	Stem Cell Research
Volume	60
DOIs	https://doi.org/10.1016/j.scr.2022.102675
Publication status	Published - Apr 2022

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10.1016/j.scr.2022.102675

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title = "Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts",

abstract = "MUi028-A human induced pluripotent stem cell (hiPSC) line was generated from normal fetal skin fibroblasts using a non-integrative reprogramming method. Reprogramming factors OCT4, SOX2, KLF4, L-MYC, and LIN28, and TP53 shRNA in three episomal vectors were delivered by electroporation. The MUi028-A cell line had embryonic stem cell characteristics with consistent expression of specific pluripotency markers and the capability of in vitro differentiation into the three germ layers. This iPSC line can be used as a control to study disease mechanisms.",

author = "Sukanya Chumchuen and Chonthicha Satirapod and Amornrat Tangprasittipap and Suradej Hongeng",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2022",

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doi = "10.1016/j.scr.2022.102675",

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AU - Chumchuen, Sukanya

AU - Satirapod, Chonthicha

AU - Tangprasittipap, Amornrat

AU - Hongeng, Suradej

PY - 2022/4

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N2 - MUi028-A human induced pluripotent stem cell (hiPSC) line was generated from normal fetal skin fibroblasts using a non-integrative reprogramming method. Reprogramming factors OCT4, SOX2, KLF4, L-MYC, and LIN28, and TP53 shRNA in three episomal vectors were delivered by electroporation. The MUi028-A cell line had embryonic stem cell characteristics with consistent expression of specific pluripotency markers and the capability of in vitro differentiation into the three germ layers. This iPSC line can be used as a control to study disease mechanisms.

AB - MUi028-A human induced pluripotent stem cell (hiPSC) line was generated from normal fetal skin fibroblasts using a non-integrative reprogramming method. Reprogramming factors OCT4, SOX2, KLF4, L-MYC, and LIN28, and TP53 shRNA in three episomal vectors were delivered by electroporation. The MUi028-A cell line had embryonic stem cell characteristics with consistent expression of specific pluripotency markers and the capability of in vitro differentiation into the three germ layers. This iPSC line can be used as a control to study disease mechanisms.

UR - https://www.scopus.com/pages/publications/85123775729

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JF - Stem Cell Research

M1 - 102675

ER -

Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts

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Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts

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