Establishing of human induced pluripotent stem cell line DMSCi002-A from the hematopoietic stem cells of a healthy male donor

Kobkaew Bumroongthai; Chonlada Yodtup; Danai Jantapalaboon; Panapat Phairoh; Supaporn Suparak; Panadda Dhepakson; Alisa Tubsuwan; Sudarat Wongkidakarn

doi:10.1016/j.scr.2025.103655

Establishing of human induced pluripotent stem cell line DMSCi002-A from the hematopoietic stem cells of a healthy male donor

Kobkaew Bumroongthai
, Chonlada Yodtup
, Danai Jantapalaboon
, Panapat Phairoh
, Supaporn Suparak
, Panadda Dhepakson
, Alisa Tubsuwan
, Sudarat Wongkidakarn

Medical Biotechnology Sciences Academic Group

Ministry of Public Health

Research output: Contribution to journal › Article › peer-review

Abstract

Using the integration-free episomal vector containing the reprogramming components OCT3/4/shp53, Sox2/KLF4, L-MYC/LIN28, and EBNA-1, hematopoietic stem cells obtained from a healthy 33-year-old man were effectively reprogrammed and turned into induced pluripotent stem cells (iPSCs). The reprogrammed iPSCs were grown without the use of feeders. They exhibited a normal karyotype, displayed pluripotency markers, and differentiated into cells from the three germ layers. This DMSCi002-A line may serve as a control for investigating disease mechanisms.

Original language	English
Article number	103655
Journal	Stem Cell Research
Volume	83
DOIs	https://doi.org/10.1016/j.scr.2025.103655
Publication status	Published - Mar 2025

Access to Document

10.1016/j.scr.2025.103655

Cite this

@article{d499fab92d924d8c8c0e9e4bb8e4de14,

title = "Establishing of human induced pluripotent stem cell line DMSCi002-A from the hematopoietic stem cells of a healthy male donor",

abstract = "Using the integration-free episomal vector containing the reprogramming components OCT3/4/shp53, Sox2/KLF4, L-MYC/LIN28, and EBNA-1, hematopoietic stem cells obtained from a healthy 33-year-old man were effectively reprogrammed and turned into induced pluripotent stem cells (iPSCs). The reprogrammed iPSCs were grown without the use of feeders. They exhibited a normal karyotype, displayed pluripotency markers, and differentiated into cells from the three germ layers. This DMSCi002-A line may serve as a control for investigating disease mechanisms.",

author = "Kobkaew Bumroongthai and Chonlada Yodtup and Danai Jantapalaboon and Panapat Phairoh and Supaporn Suparak and Panadda Dhepakson and Alisa Tubsuwan and Sudarat Wongkidakarn",

note = "Publisher Copyright: {\textcopyright} 2025",

year = "2025",

month = mar,

doi = "10.1016/j.scr.2025.103655",

language = "English",

volume = "83",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Establishing of human induced pluripotent stem cell line DMSCi002-A from the hematopoietic stem cells of a healthy male donor

AU - Bumroongthai, Kobkaew

AU - Yodtup, Chonlada

AU - Jantapalaboon, Danai

AU - Phairoh, Panapat

AU - Suparak, Supaporn

AU - Dhepakson, Panadda

AU - Tubsuwan, Alisa

AU - Wongkidakarn, Sudarat

PY - 2025/3

Y1 - 2025/3

N2 - Using the integration-free episomal vector containing the reprogramming components OCT3/4/shp53, Sox2/KLF4, L-MYC/LIN28, and EBNA-1, hematopoietic stem cells obtained from a healthy 33-year-old man were effectively reprogrammed and turned into induced pluripotent stem cells (iPSCs). The reprogrammed iPSCs were grown without the use of feeders. They exhibited a normal karyotype, displayed pluripotency markers, and differentiated into cells from the three germ layers. This DMSCi002-A line may serve as a control for investigating disease mechanisms.

AB - Using the integration-free episomal vector containing the reprogramming components OCT3/4/shp53, Sox2/KLF4, L-MYC/LIN28, and EBNA-1, hematopoietic stem cells obtained from a healthy 33-year-old man were effectively reprogrammed and turned into induced pluripotent stem cells (iPSCs). The reprogrammed iPSCs were grown without the use of feeders. They exhibited a normal karyotype, displayed pluripotency markers, and differentiated into cells from the three germ layers. This DMSCi002-A line may serve as a control for investigating disease mechanisms.

UR - https://www.scopus.com/pages/publications/85214299343

U2 - 10.1016/j.scr.2025.103655

DO - 10.1016/j.scr.2025.103655

M3 - Article

C2 - 39778439

AN - SCOPUS:85214299343

SN - 1873-5061

VL - 83

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 103655

ER -

Establishing of human induced pluripotent stem cell line DMSCi002-A from the hematopoietic stem cells of a healthy male donor

Abstract

Access to Document

Other files and links

Fingerprint

Cite this