Dosage compensation of Z sex chromosome genes in avian fibroblast cells

Ruslan Deviatiiarov; Hiroki Nagai; Galym Ismagulov; Anastasia Stupina; Kazuhiro Wada; Shinji Ide; Noriyuki Toji; Heng Zhang; Woranop Sukparangsi; Sittipon Intarapat; Oleg Gusev; Guojun Sheng

doi:10.1186/s13059-023-03055-z

Dosage compensation of Z sex chromosome genes in avian fibroblast cells

Ruslan Deviatiiarov
, Hiroki Nagai
, Galym Ismagulov
, Anastasia Stupina
, Kazuhiro Wada
, Shinji Ide
, Noriyuki Toji
, Heng Zhang
, Woranop Sukparangsi
, Sittipon Intarapat
, Oleg Gusev
, Guojun Sheng

Kumamoto University
Kazan Federal University
Juntendo University Graduate School of Medicine
Life Improvement by Future Technologies (LIFT) Center
Hokkaido University
Kumamoto City Zoo and Botanical Garden
Burapha University
Mahidol University

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

In birds, sex is genetically determined; however, the molecular mechanism is not well-understood. The avian Z sex chromosome (chrZ) lacks whole chromosome inactivation, in contrast to the mammalian chrX. To investigate chrZ dosage compensation and its role in sex specification, we use a highly quantitative method and analyze transcriptional activities of male and female fibroblast cells from seven bird species. Our data indicate that three fourths of chrZ genes are strictly compensated across Aves, similar to mammalian chrX. We also present a complete list of non-compensated chrZ genes and identify Ribosomal Protein S6 (RPS6) as a conserved sex-dimorphic gene in birds.

Original language	English
Article number	213
Journal	Genome Biology
Volume	24
Issue number	1
DOIs	https://doi.org/10.1186/s13059-023-03055-z
Publication status	Published - Dec 2023
Externally published	Yes

Keywords

Birds
CAGE (cap analysis of gene expression)
CASI (cell autonomous sex identity)
Dosage compensation
Fibroblast
RPS6 (ribosomal protein S6)
Sex chromosome
Sex determination
TSS (transcription start site)

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10.1186/s13059-023-03055-z

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title = "Dosage compensation of Z sex chromosome genes in avian fibroblast cells",

abstract = "In birds, sex is genetically determined; however, the molecular mechanism is not well-understood. The avian Z sex chromosome (chrZ) lacks whole chromosome inactivation, in contrast to the mammalian chrX. To investigate chrZ dosage compensation and its role in sex specification, we use a highly quantitative method and analyze transcriptional activities of male and female fibroblast cells from seven bird species. Our data indicate that three fourths of chrZ genes are strictly compensated across Aves, similar to mammalian chrX. We also present a complete list of non-compensated chrZ genes and identify Ribosomal Protein S6 (RPS6) as a conserved sex-dimorphic gene in birds.",

keywords = "Birds, CAGE (cap analysis of gene expression), CASI (cell autonomous sex identity), Dosage compensation, Fibroblast, RPS6 (ribosomal protein S6), Sex chromosome, Sex determination, TSS (transcription start site)",

author = "Ruslan Deviatiiarov and Hiroki Nagai and Galym Ismagulov and Anastasia Stupina and Kazuhiro Wada and Shinji Ide and Noriyuki Toji and Heng Zhang and Woranop Sukparangsi and Sittipon Intarapat and Oleg Gusev and Guojun Sheng",

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year = "2023",

month = dec,

doi = "10.1186/s13059-023-03055-z",

language = "English",

volume = "24",

journal = "Genome Biology",

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TY - JOUR

T1 - Dosage compensation of Z sex chromosome genes in avian fibroblast cells

AU - Deviatiiarov, Ruslan

AU - Nagai, Hiroki

AU - Ismagulov, Galym

AU - Stupina, Anastasia

AU - Wada, Kazuhiro

AU - Ide, Shinji

AU - Toji, Noriyuki

AU - Zhang, Heng

AU - Sukparangsi, Woranop

AU - Intarapat, Sittipon

AU - Gusev, Oleg

AU - Sheng, Guojun

PY - 2023/12

Y1 - 2023/12

N2 - In birds, sex is genetically determined; however, the molecular mechanism is not well-understood. The avian Z sex chromosome (chrZ) lacks whole chromosome inactivation, in contrast to the mammalian chrX. To investigate chrZ dosage compensation and its role in sex specification, we use a highly quantitative method and analyze transcriptional activities of male and female fibroblast cells from seven bird species. Our data indicate that three fourths of chrZ genes are strictly compensated across Aves, similar to mammalian chrX. We also present a complete list of non-compensated chrZ genes and identify Ribosomal Protein S6 (RPS6) as a conserved sex-dimorphic gene in birds.

AB - In birds, sex is genetically determined; however, the molecular mechanism is not well-understood. The avian Z sex chromosome (chrZ) lacks whole chromosome inactivation, in contrast to the mammalian chrX. To investigate chrZ dosage compensation and its role in sex specification, we use a highly quantitative method and analyze transcriptional activities of male and female fibroblast cells from seven bird species. Our data indicate that three fourths of chrZ genes are strictly compensated across Aves, similar to mammalian chrX. We also present a complete list of non-compensated chrZ genes and identify Ribosomal Protein S6 (RPS6) as a conserved sex-dimorphic gene in birds.

KW - Birds

KW - CAGE (cap analysis of gene expression)

KW - CASI (cell autonomous sex identity)

KW - Dosage compensation

KW - Fibroblast

KW - RPS6 (ribosomal protein S6)

KW - Sex chromosome

KW - Sex determination

KW - TSS (transcription start site)

UR - https://www.scopus.com/pages/publications/85171808539

U2 - 10.1186/s13059-023-03055-z

DO - 10.1186/s13059-023-03055-z

M3 - Article

C2 - 37730643

AN - SCOPUS:85171808539

SN - 1474-7596

VL - 24

JO - Genome Biology

JF - Genome Biology

IS - 1

M1 - 213

ER -

Dosage compensation of Z sex chromosome genes in avian fibroblast cells

Abstract

Keywords

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