@inbook{ebbf04f4e1b94ae9b171d03c6d110133,
title = "Direct Sequencing of RNA and RNA Modification Identification Using Nanopore",
abstract = "Direct RNA sequencing (dRNA-seq) simultaneously enables the detection of RNA modifications and characterization of full-length transcripts. In principle, full-length native RNA molecule is translocated through the nanopore by a motor protein while a sensor measures ionic current shifts. Then, the current shifts are interpreted by an algorithm that turn out to RNA sequence. Currently, the standard protocol of dRNA-seq provided by Oxford Nanopore Technologies (ONT) allows to directly ligate and sequence only polyadenylated RNA (poly(A) RNA). Here, we describe a method of dRNA-seq that can be applied for both poly(A) RNA and non-poly(A) tailed-RNA.",
keywords = "Direct RNA sequencing, Epitranscriptome, Long-read, Methylation, Modification, Nanopore, Native, RNA, Sequencing Technology, Transcriptome",
author = "Thidathip Wongsurawat and Piroon Jenjaroenpun and Intawat Nookaew",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2257-5\_5",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "71--77",
booktitle = "Methods in Molecular Biology",
}