Development of ochratoxin A detection based on electrochemical sensor using Auball labels

Aims: The present study was aimed to develop a highly sensitive and rapid method for ochratoxin A (OTA) detection. Methodology and results: In this study, an electrochemical peptidesensor for OTA detection was developed using numerous number of Au particles coated on the surface of silica particle (Au-ball). Moreover, this assay was performed in 384 well plate, so the multiple detections was done. The synthesized silica particle was spherical in shape and size was 275±17 nm. After coated with Au layer, size of Au-ball was 280±14 nm. Au particles loaded can be taken up resulting in approx. 1×10⁷ Au³⁺ molecules per silica particle. Moreover, the optimization conditions were studied. The limit of detection of this assay showed as low as 2 ppb. Conclusion, significance and impact study: This platform showed rapid, sensitive and specific to ochratoxin A detection. In spite of these advantages, this Au-ball based peptidesensors is suitable to use in the detection of ochratoxin A contaminated in coffee or seed industry.