Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein

Benyakarn Pongkitwitoon; Seiichi Sakamoto; Hiroyuki Tanaka; Ryota Tsuchihashi; Junei Kinjo; Satoshi Morimoto; Waraporn Putalun

doi:10.1007/s11418-010-0448-z

Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein

Benyakarn Pongkitwitoon
, Seiichi Sakamoto
, Hiroyuki Tanaka
, Ryota Tsuchihashi
, Junei Kinjo
, Satoshi Morimoto
, Waraporn Putalun

Khon Kaen University
Kyushu University
Fukuoka University

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin (8-C-glucosyl-4′,7-dihydroxyisoflavone) and its aglycone, daidzein, as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these isoflavonoids, an enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against puerarin. Puerarin was conjugated with bovine serum albumin (BSA) to be used as an immunogen for production of PAb from a rabbit. The PAb has been shown to be specific to puerarin and its aglycone, daidzein. An ELISA was then performed using anti-puerarin PAb in the range of 6.10 ng/ml-1.56 μg/ml of puerarin. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for determining puerarin and daidzein in plant samples.

Original language	English
Pages (from-to)	31-36
Number of pages	6
Journal	Journal of Natural Medicines
Volume	65
Issue number	1
DOIs	https://doi.org/10.1007/s11418-010-0448-z
Publication status	Published - Jan 2011
Externally published	Yes

Keywords

Daidzein
Enzyme-linked immunosorbent assay (ELISA)
Polyclonal antibody
Pueraria candollei
Puerarin

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10.1007/s11418-010-0448-z

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title = "Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein",

abstract = "Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin (8-C-glucosyl-4′,7-dihydroxyisoflavone) and its aglycone, daidzein, as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these isoflavonoids, an enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against puerarin. Puerarin was conjugated with bovine serum albumin (BSA) to be used as an immunogen for production of PAb from a rabbit. The PAb has been shown to be specific to puerarin and its aglycone, daidzein. An ELISA was then performed using anti-puerarin PAb in the range of 6.10 ng/ml-1.56 μg/ml of puerarin. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for determining puerarin and daidzein in plant samples.",

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author = "Benyakarn Pongkitwitoon and Seiichi Sakamoto and Hiroyuki Tanaka and Ryota Tsuchihashi and Junei Kinjo and Satoshi Morimoto and Waraporn Putalun",

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doi = "10.1007/s11418-010-0448-z",

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AU - Pongkitwitoon, Benyakarn

AU - Sakamoto, Seiichi

AU - Tanaka, Hiroyuki

AU - Tsuchihashi, Ryota

AU - Kinjo, Junei

AU - Morimoto, Satoshi

AU - Putalun, Waraporn

PY - 2011/1

Y1 - 2011/1

N2 - Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin (8-C-glucosyl-4′,7-dihydroxyisoflavone) and its aglycone, daidzein, as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these isoflavonoids, an enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against puerarin. Puerarin was conjugated with bovine serum albumin (BSA) to be used as an immunogen for production of PAb from a rabbit. The PAb has been shown to be specific to puerarin and its aglycone, daidzein. An ELISA was then performed using anti-puerarin PAb in the range of 6.10 ng/ml-1.56 μg/ml of puerarin. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for determining puerarin and daidzein in plant samples.

AB - Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin (8-C-glucosyl-4′,7-dihydroxyisoflavone) and its aglycone, daidzein, as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these isoflavonoids, an enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal antibody (PAb) against puerarin. Puerarin was conjugated with bovine serum albumin (BSA) to be used as an immunogen for production of PAb from a rabbit. The PAb has been shown to be specific to puerarin and its aglycone, daidzein. An ELISA was then performed using anti-puerarin PAb in the range of 6.10 ng/ml-1.56 μg/ml of puerarin. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for determining puerarin and daidzein in plant samples.

KW - Daidzein

KW - Enzyme-linked immunosorbent assay (ELISA)

KW - Polyclonal antibody

KW - Pueraria candollei

KW - Puerarin

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M3 - Article

C2 - 20658197

AN - SCOPUS:78651387053

SN - 1340-3443

VL - 65

SP - 31

EP - 36

JO - Journal of Natural Medicines

JF - Journal of Natural Medicines

IS - 1

ER -

Development of an enzyme-linked immunosorbent assay to determine puerarin and its aglycone daidzein

Abstract

Keywords

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