Development and evaluation of a one-tube seminested PCR assay for the detection and identification of Penicillium marneffei

C. Prariyachatigul; A. Chaiprasert; K. Geenkajorn; R. Kappe; C. Chuchottaworn; S. Termsetjaroen; S. Srimuang

doi:10.1046/j.0933-7407.2003.00939.x

Development and evaluation of a one-tube seminested PCR assay for the detection and identification of Penicillium marneffei

C. Prariyachatigul
, A. Chaiprasert
, K. Geenkajorn
, R. Kappe
, C. Chuchottaworn
, S. Termsetjaroen
, S. Srimuang

Department of Microbiology

Khon Kaen University
Siriraj Hospital
Helios Medical Center
Central Hospital
Ramathibodi Hospital

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

A one-tube seminested polymerase chain reaction (PCR) assay was developed to detect and identify Penicillium marneffei DNA coding for 18S rRNA both from purified DNA and from clinical samples. DNA from 120 strains of organisms and 19 blood samples from AIDS patients was amplified with F3, CPL1 and PM primers. Under optimized conditions, these primers detected 100% specifically amplified products of 251 and 331 bp from all P. marneffei DNA preparations (47 strains) and from two blood samples of AIDS patients suspected to suffer from penicilliosis marneffei. The assay was sensitive to detect as little as 10 pg purified DNA, which is equivalent to 250 cells. This PCR assay might be useful as an alternative test, if a rapid diagnosis of penicilliosis marneffei is needed.

Original language	English
Pages (from-to)	447-454
Number of pages	8
Journal	Mycoses
Volume	46
Issue number	11-12
DOIs	https://doi.org/10.1046/j.0933-7407.2003.00939.x
Publication status	Published - 2003

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

18S rRNA
Detection
One-tube seminested polymerase chain reaction assay
Penicillium marneffei

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10.1046/j.0933-7407.2003.00939.x

Cite this

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title = "Development and evaluation of a one-tube seminested PCR assay for the detection and identification of Penicillium marneffei",

abstract = "A one-tube seminested polymerase chain reaction (PCR) assay was developed to detect and identify Penicillium marneffei DNA coding for 18S rRNA both from purified DNA and from clinical samples. DNA from 120 strains of organisms and 19 blood samples from AIDS patients was amplified with F3, CPL1 and PM primers. Under optimized conditions, these primers detected 100\% specifically amplified products of 251 and 331 bp from all P. marneffei DNA preparations (47 strains) and from two blood samples of AIDS patients suspected to suffer from penicilliosis marneffei. The assay was sensitive to detect as little as 10 pg purified DNA, which is equivalent to 250 cells. This PCR assay might be useful as an alternative test, if a rapid diagnosis of penicilliosis marneffei is needed.",

keywords = "18S rRNA, Detection, One-tube seminested polymerase chain reaction assay, Penicillium marneffei",

author = "C. Prariyachatigul and A. Chaiprasert and K. Geenkajorn and R. Kappe and C. Chuchottaworn and S. Termsetjaroen and S. Srimuang",

year = "2003",

doi = "10.1046/j.0933-7407.2003.00939.x",

language = "English",

volume = "46",

pages = "447--454",

journal = "Mycoses",

issn = "0933-7407",

publisher = "Wiley-Blackwell Publishing Ltd",

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TY - JOUR

T1 - Development and evaluation of a one-tube seminested PCR assay for the detection and identification of Penicillium marneffei

AU - Prariyachatigul, C.

AU - Chaiprasert, A.

AU - Geenkajorn, K.

AU - Kappe, R.

AU - Chuchottaworn, C.

AU - Termsetjaroen, S.

AU - Srimuang, S.

PY - 2003

Y1 - 2003

N2 - A one-tube seminested polymerase chain reaction (PCR) assay was developed to detect and identify Penicillium marneffei DNA coding for 18S rRNA both from purified DNA and from clinical samples. DNA from 120 strains of organisms and 19 blood samples from AIDS patients was amplified with F3, CPL1 and PM primers. Under optimized conditions, these primers detected 100% specifically amplified products of 251 and 331 bp from all P. marneffei DNA preparations (47 strains) and from two blood samples of AIDS patients suspected to suffer from penicilliosis marneffei. The assay was sensitive to detect as little as 10 pg purified DNA, which is equivalent to 250 cells. This PCR assay might be useful as an alternative test, if a rapid diagnosis of penicilliosis marneffei is needed.

AB - A one-tube seminested polymerase chain reaction (PCR) assay was developed to detect and identify Penicillium marneffei DNA coding for 18S rRNA both from purified DNA and from clinical samples. DNA from 120 strains of organisms and 19 blood samples from AIDS patients was amplified with F3, CPL1 and PM primers. Under optimized conditions, these primers detected 100% specifically amplified products of 251 and 331 bp from all P. marneffei DNA preparations (47 strains) and from two blood samples of AIDS patients suspected to suffer from penicilliosis marneffei. The assay was sensitive to detect as little as 10 pg purified DNA, which is equivalent to 250 cells. This PCR assay might be useful as an alternative test, if a rapid diagnosis of penicilliosis marneffei is needed.

KW - 18S rRNA

KW - Detection

KW - One-tube seminested polymerase chain reaction assay

KW - Penicillium marneffei

UR - https://www.scopus.com/pages/publications/0346216148

U2 - 10.1046/j.0933-7407.2003.00939.x

DO - 10.1046/j.0933-7407.2003.00939.x

M3 - Article

C2 - 14641616

AN - SCOPUS:0346216148

SN - 0933-7407

VL - 46

SP - 447

EP - 454

JO - Mycoses

JF - Mycoses

IS - 11-12

ER -

Development and evaluation of a one-tube seminested PCR assay for the detection and identification of Penicillium marneffei

Abstract

UN SDGs

Keywords

Access to Document

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