Determination of discriminatory power of genetic markers used for genotyping giardia duodenalis

Suradej Siripattanapipong; Saovanee Leelayoova; Mathirut Mungthin; Rc Andrew Thompson; Parima Boontanom; Wilai Saksirisamphant; Peerapan Tan-Ariya

Determination of discriminatory power of genetic markers used for genotyping giardia duodenalis

Suradej Siripattanapipong
, Saovanee Leelayoova
, Mathirut Mungthin
, Rc Andrew Thompson
, Parima Boontanom
, Wilai Saksirisamphant
, Peerapan Tan-Ariya

Mahidol University
Phramongkutklao Hospital
Murdoch University
Chulalongkorn University

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Small subunit ribosomal DNA (SSU-rDNA), glutamate dehydrogenase (gdh), β-giardin, triosephosphate isomerase (tpi), and elongation factor 1-alpha (ef1-α) genes are useful genetic markers for genotypic analysis of the intestinal protozoan, Giardia duodenalis (syn. G. lamblia, G. intestinalis), the cause of enteric disease in humans. To quantitatively compare the discriminatory power of these loci, 43 fecal samples were collected from central, northern and eastern Thailand and G. duodenalis specimens were analyzed using PCR-based genotyping and subcloning methods. Approximately equal prevalence of assemblage A (21) and B (22) were present among these populations. Analysis of Simpson's index and Wallace coefficient values from assemblage B isolates together with the data obtained from GenBank showed that the combination of two loci provides a higher discrimination power for subgenotyping G. duodenalis than using any single locus.

Original language	English
Pages (from-to)	764-771
Number of pages	8
Journal	Southeast Asian Journal of Tropical Medicine and Public Health
Volume	42
Issue number	4
Publication status	Published - Jul 2011
Externally published	Yes

Keywords

Discriminatory power
Genetic diversity
Giardia duodenalis
Multilocus typing

Cite this

@article{8d5b9270a0e644ddb313020b9a966125,

title = "Determination of discriminatory power of genetic markers used for genotyping giardia duodenalis",

abstract = "Small subunit ribosomal DNA (SSU-rDNA), glutamate dehydrogenase (gdh), β-giardin, triosephosphate isomerase (tpi), and elongation factor 1-alpha (ef1-α) genes are useful genetic markers for genotypic analysis of the intestinal protozoan, Giardia duodenalis (syn. G. lamblia, G. intestinalis), the cause of enteric disease in humans. To quantitatively compare the discriminatory power of these loci, 43 fecal samples were collected from central, northern and eastern Thailand and G. duodenalis specimens were analyzed using PCR-based genotyping and subcloning methods. Approximately equal prevalence of assemblage A (21) and B (22) were present among these populations. Analysis of Simpson's index and Wallace coefficient values from assemblage B isolates together with the data obtained from GenBank showed that the combination of two loci provides a higher discrimination power for subgenotyping G. duodenalis than using any single locus.",

keywords = "Discriminatory power, Genetic diversity, Giardia duodenalis, Multilocus typing",

author = "Suradej Siripattanapipong and Saovanee Leelayoova and Mathirut Mungthin and Thompson, \{Rc Andrew\} and Parima Boontanom and Wilai Saksirisamphant and Peerapan Tan-Ariya",

year = "2011",

month = jul,

language = "English",

volume = "42",

pages = "764--771",

journal = "Southeast Asian Journal of Tropical Medicine and Public Health",

issn = "0125-1562",

publisher = "SEAMEO TROPMED Network",

number = "4",

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TY - JOUR

T1 - Determination of discriminatory power of genetic markers used for genotyping giardia duodenalis

AU - Siripattanapipong, Suradej

AU - Leelayoova, Saovanee

AU - Mungthin, Mathirut

AU - Thompson, Rc Andrew

AU - Boontanom, Parima

AU - Saksirisamphant, Wilai

AU - Tan-Ariya, Peerapan

PY - 2011/7

Y1 - 2011/7

N2 - Small subunit ribosomal DNA (SSU-rDNA), glutamate dehydrogenase (gdh), β-giardin, triosephosphate isomerase (tpi), and elongation factor 1-alpha (ef1-α) genes are useful genetic markers for genotypic analysis of the intestinal protozoan, Giardia duodenalis (syn. G. lamblia, G. intestinalis), the cause of enteric disease in humans. To quantitatively compare the discriminatory power of these loci, 43 fecal samples were collected from central, northern and eastern Thailand and G. duodenalis specimens were analyzed using PCR-based genotyping and subcloning methods. Approximately equal prevalence of assemblage A (21) and B (22) were present among these populations. Analysis of Simpson's index and Wallace coefficient values from assemblage B isolates together with the data obtained from GenBank showed that the combination of two loci provides a higher discrimination power for subgenotyping G. duodenalis than using any single locus.

AB - Small subunit ribosomal DNA (SSU-rDNA), glutamate dehydrogenase (gdh), β-giardin, triosephosphate isomerase (tpi), and elongation factor 1-alpha (ef1-α) genes are useful genetic markers for genotypic analysis of the intestinal protozoan, Giardia duodenalis (syn. G. lamblia, G. intestinalis), the cause of enteric disease in humans. To quantitatively compare the discriminatory power of these loci, 43 fecal samples were collected from central, northern and eastern Thailand and G. duodenalis specimens were analyzed using PCR-based genotyping and subcloning methods. Approximately equal prevalence of assemblage A (21) and B (22) were present among these populations. Analysis of Simpson's index and Wallace coefficient values from assemblage B isolates together with the data obtained from GenBank showed that the combination of two loci provides a higher discrimination power for subgenotyping G. duodenalis than using any single locus.

KW - Discriminatory power

KW - Genetic diversity

KW - Giardia duodenalis

KW - Multilocus typing

UR - https://www.scopus.com/pages/publications/80054913809

M3 - Article

AN - SCOPUS:80054913809

SN - 0125-1562

VL - 42

SP - 764

EP - 771

JO - Southeast Asian Journal of Tropical Medicine and Public Health

JF - Southeast Asian Journal of Tropical Medicine and Public Health

IS - 4

ER -

Determination of discriminatory power of genetic markers used for genotyping giardia duodenalis

Abstract

Keywords

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