Derivation of human embryonic stem cell line MUSIe001-A from an embryo with homozygous α0-thalassemia (SEA deletion)

Chuti Laowtammathron; Pimjai Chingsuwanrote; Roungsin Choavaratana; Suphadtra Phornwilardsiri; Ketsara Sitthirit; Chidchanok Kaewjunun; Orawan Makemaharn; Papussorn Terbto; Supaporn Waeteekul; Chanchao Lorthongpanich; Yaowalak U-pratya; Pimonwan Srisook; Pakpoom Kheolamai; Surapol Issaragrisil

doi:10.1016/j.scr.2019.101695

Derivation of human embryonic stem cell line MUSIe001-A from an embryo with homozygous α⁰-thalassemia (SEA deletion)

Chuti Laowtammathron
, Pimjai Chingsuwanrote
, Roungsin Choavaratana
, Suphadtra Phornwilardsiri
, Ketsara Sitthirit
, Chidchanok Kaewjunun
, Orawan Makemaharn
, Papussorn Terbto
, Supaporn Waeteekul
, Chanchao Lorthongpanich
, Yaowalak U-pratya
, Pimonwan Srisook
, Pakpoom Kheolamai
, Surapol Issaragrisil

Office of the Dean

Siriraj Hospital
Faculty of Medicine, Thammasat University

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

MUSIe001-A cell line was derived from a Southeast Asian (SEA) type deletion α⁰-thalassemia embryo. The SEA deletion embryo was donated for research with informed consent. This cell line shows normal hESC morphology, expresses all pluripotent markers, and has the potential to differentiate into all three germ layers in vitro and in vivo. The MUSIe001-A line has normal karyotype and is free from mycoplasma contamination. PCR analysis confirmed the MUSIe001-A cell line to be a SEA type deletion. MUSIe001-A is a valuable proof of principle model for gene therapy that will facilitate the development of new treatments for affected foetuses.

Original language	English
Article number	101695
Journal	Stem Cell Research
Volume	43
DOIs	https://doi.org/10.1016/j.scr.2019.101695
Publication status	Published - Mar 2020

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

Access to Document

10.1016/j.scr.2019.101695

Cite this

Laowtammathron, C., Chingsuwanrote, P., Choavaratana, R., Phornwilardsiri, S., Sitthirit, K., Kaewjunun, C., Makemaharn, O., Terbto, P., Waeteekul, S., Lorthongpanich, C., U-pratya, Y., Srisook, P., Kheolamai, P., & Issaragrisil, S. (2020). Derivation of human embryonic stem cell line MUSIe001-A from an embryo with homozygous α⁰-thalassemia (SEA deletion). Stem Cell Research, 43, Article 101695. https://doi.org/10.1016/j.scr.2019.101695

@article{ca3256c0539e469186364998f7678f1b,

title = "Derivation of human embryonic stem cell line MUSIe001-A from an embryo with homozygous α0-thalassemia (SEA deletion)",

abstract = "MUSIe001-A cell line was derived from a Southeast Asian (SEA) type deletion α0-thalassemia embryo. The SEA deletion embryo was donated for research with informed consent. This cell line shows normal hESC morphology, expresses all pluripotent markers, and has the potential to differentiate into all three germ layers in vitro and in vivo. The MUSIe001-A line has normal karyotype and is free from mycoplasma contamination. PCR analysis confirmed the MUSIe001-A cell line to be a SEA type deletion. MUSIe001-A is a valuable proof of principle model for gene therapy that will facilitate the development of new treatments for affected foetuses.",

author = "Chuti Laowtammathron and Pimjai Chingsuwanrote and Roungsin Choavaratana and Suphadtra Phornwilardsiri and Ketsara Sitthirit and Chidchanok Kaewjunun and Orawan Makemaharn and Papussorn Terbto and Supaporn Waeteekul and Chanchao Lorthongpanich and Yaowalak U-pratya and Pimonwan Srisook and Pakpoom Kheolamai and Surapol Issaragrisil",

note = "Publisher Copyright: {\textcopyright} 2020 The Authors",

year = "2020",

month = mar,

doi = "10.1016/j.scr.2019.101695",

language = "English",

volume = "43",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier B.V.",

}

Laowtammathron, C, Chingsuwanrote, P, Choavaratana, R, Phornwilardsiri, S, Sitthirit, K, Kaewjunun, C, Makemaharn, O, Terbto, P, Waeteekul, S, Lorthongpanich, C, U-pratya, Y, Srisook, P, Kheolamai, P & Issaragrisil, S 2020, 'Derivation of human embryonic stem cell line MUSIe001-A from an embryo with homozygous α⁰-thalassemia (SEA deletion)', Stem Cell Research, vol. 43, 101695. https://doi.org/10.1016/j.scr.2019.101695

TY - JOUR

T1 - Derivation of human embryonic stem cell line MUSIe001-A from an embryo with homozygous α0-thalassemia (SEA deletion)

AU - Laowtammathron, Chuti

AU - Chingsuwanrote, Pimjai

AU - Choavaratana, Roungsin

AU - Phornwilardsiri, Suphadtra

AU - Sitthirit, Ketsara

AU - Kaewjunun, Chidchanok

AU - Makemaharn, Orawan

AU - Terbto, Papussorn

AU - Waeteekul, Supaporn

AU - Lorthongpanich, Chanchao

AU - U-pratya, Yaowalak

AU - Srisook, Pimonwan

AU - Kheolamai, Pakpoom

AU - Issaragrisil, Surapol

PY - 2020/3

Y1 - 2020/3

N2 - MUSIe001-A cell line was derived from a Southeast Asian (SEA) type deletion α0-thalassemia embryo. The SEA deletion embryo was donated for research with informed consent. This cell line shows normal hESC morphology, expresses all pluripotent markers, and has the potential to differentiate into all three germ layers in vitro and in vivo. The MUSIe001-A line has normal karyotype and is free from mycoplasma contamination. PCR analysis confirmed the MUSIe001-A cell line to be a SEA type deletion. MUSIe001-A is a valuable proof of principle model for gene therapy that will facilitate the development of new treatments for affected foetuses.

AB - MUSIe001-A cell line was derived from a Southeast Asian (SEA) type deletion α0-thalassemia embryo. The SEA deletion embryo was donated for research with informed consent. This cell line shows normal hESC morphology, expresses all pluripotent markers, and has the potential to differentiate into all three germ layers in vitro and in vivo. The MUSIe001-A line has normal karyotype and is free from mycoplasma contamination. PCR analysis confirmed the MUSIe001-A cell line to be a SEA type deletion. MUSIe001-A is a valuable proof of principle model for gene therapy that will facilitate the development of new treatments for affected foetuses.

UR - https://www.scopus.com/pages/publications/85077687697

U2 - 10.1016/j.scr.2019.101695

DO - 10.1016/j.scr.2019.101695

M3 - Article

C2 - 31945613

AN - SCOPUS:85077687697

SN - 1873-5061

VL - 43

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 101695

ER -

Derivation of human embryonic stem cell line MUSIe001-A from an embryo with homozygous α⁰-thalassemia (SEA deletion)

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this