Crystallization and preliminary X-ray diffraction study of mammalian mitochondrial seryl-tRNA synthetase

Sarin Chimnaronk; Mads Gravers Jeppesen; Nobukazu Shimada; Tsutomu Suzuki; Jens Nyborg; Kimitsuna Watanabe

doi:10.1107/S0907444904011217

Crystallization and preliminary X-ray diffraction study of mammalian mitochondrial seryl-tRNA synthetase

Sarin Chimnaronk
, Mads Gravers Jeppesen
, Nobukazu Shimada
, Tsutomu Suzuki
, Jens Nyborg
, Kimitsuna Watanabe

University of Tokyo
University of Aarhus

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The mitochondrial seryl-tRNA synthetase (mt SerRS) from Bos taurus was overexpressed in Escherichia coli and crystallized using the sitting-drop vapour-diffusion method. Crystals grew in a very narrow range of conditions using PEG 8000 as precipitant at room temperature. An appropriate concentration of lithium sulfate was critical for crystal nucleation. Crystals diffracted well beyond a resolution of 1.6 Å and were found to belong to the orthorhombic space group C222₁, with unit-cell parameters a = 79.89, b = 230.42, c = 135.60 Å. There is one dimer (M_r ≃ 113 kDa) in the asymmetric unit, with a solvent content of 55%. Efforts to solve the phase problem by molecular replacement are under way.

Original language	English
Pages (from-to)	1319-1322
Number of pages	4
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	60
Issue number	7
DOIs	https://doi.org/10.1107/S0907444904011217
Publication status	Published - Jul 2004
Externally published	Yes

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title = "Crystallization and preliminary X-ray diffraction study of mammalian mitochondrial seryl-tRNA synthetase",

abstract = "The mitochondrial seryl-tRNA synthetase (mt SerRS) from Bos taurus was overexpressed in Escherichia coli and crystallized using the sitting-drop vapour-diffusion method. Crystals grew in a very narrow range of conditions using PEG 8000 as precipitant at room temperature. An appropriate concentration of lithium sulfate was critical for crystal nucleation. Crystals diffracted well beyond a resolution of 1.6 {\AA} and were found to belong to the orthorhombic space group C2221, with unit-cell parameters a = 79.89, b = 230.42, c = 135.60 {\AA}. There is one dimer (Mr ≃ 113 kDa) in the asymmetric unit, with a solvent content of 55\%. Efforts to solve the phase problem by molecular replacement are under way.",

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T1 - Crystallization and preliminary X-ray diffraction study of mammalian mitochondrial seryl-tRNA synthetase

AU - Chimnaronk, Sarin

AU - Jeppesen, Mads Gravers

AU - Shimada, Nobukazu

AU - Suzuki, Tsutomu

AU - Nyborg, Jens

AU - Watanabe, Kimitsuna

PY - 2004/7

Y1 - 2004/7

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AB - The mitochondrial seryl-tRNA synthetase (mt SerRS) from Bos taurus was overexpressed in Escherichia coli and crystallized using the sitting-drop vapour-diffusion method. Crystals grew in a very narrow range of conditions using PEG 8000 as precipitant at room temperature. An appropriate concentration of lithium sulfate was critical for crystal nucleation. Crystals diffracted well beyond a resolution of 1.6 Å and were found to belong to the orthorhombic space group C2221, with unit-cell parameters a = 79.89, b = 230.42, c = 135.60 Å. There is one dimer (Mr ≃ 113 kDa) in the asymmetric unit, with a solvent content of 55%. Efforts to solve the phase problem by molecular replacement are under way.

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JF - Acta Crystallographica Section D: Biological Crystallography

IS - 7

ER -

Crystallization and preliminary X-ray diffraction study of mammalian mitochondrial seryl-tRNA synthetase

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