CRISPR/Cas9 mediated approach to generate YAP-depleted human embryonic stem cell line (MUSIe002-A-1)

Sujittra Khampang; Nittaya Jiamvoraphong; Chuti Laowtammathron; Chanchao Lorthongpanich; Phatchanat Klaihmon; Pimonwan Srisook; Xing Kang; Sudjit Luanpitpong; Yaowalak U-pratya; Surapol Issaragrisil

doi:10.1016/j.scr.2022.102990

CRISPR/Cas9 mediated approach to generate YAP-depleted human embryonic stem cell line (MUSIe002-A-1)

Sujittra Khampang
, Nittaya Jiamvoraphong
, Chuti Laowtammathron
, Chanchao Lorthongpanich
, Phatchanat Klaihmon
, Pimonwan Srisook
, Xing Kang
, Sudjit Luanpitpong
, Yaowalak U-pratya
, Surapol Issaragrisil

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Siriraj Hospital
Wattanosoth Hospital

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1 Citation (Scopus)

Abstract

Yes-associated protein (YAP), an important effector protein of the Hippo signaling pathway, acts as a molecular switch in controlling cell proliferation and apoptosis. In this study, a YAP-targeted isogenic sub-clone of the MUSIe002-A was generated, designated as MUSIe002-A-1. The MUSIe002-1 cell line had normal pluripotent stem cell characteristics and karyotype. Its ability to differentiate into three germ layers was confirmed. As reduction of YAP does not disturb the pluripotency of hESCs, this cell line serves as a valuable model to extrapolate the functional role of YAP in stem cell biology and its applications.

Original language	English
Article number	102990
Journal	Stem Cell Research
Volume	66
DOIs	https://doi.org/10.1016/j.scr.2022.102990
Publication status	Published - Feb 2023

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title = "CRISPR/Cas9 mediated approach to generate YAP-depleted human embryonic stem cell line (MUSIe002-A-1)",

abstract = "Yes-associated protein (YAP), an important effector protein of the Hippo signaling pathway, acts as a molecular switch in controlling cell proliferation and apoptosis. In this study, a YAP-targeted isogenic sub-clone of the MUSIe002-A was generated, designated as MUSIe002-A-1. The MUSIe002-1 cell line had normal pluripotent stem cell characteristics and karyotype. Its ability to differentiate into three germ layers was confirmed. As reduction of YAP does not disturb the pluripotency of hESCs, this cell line serves as a valuable model to extrapolate the functional role of YAP in stem cell biology and its applications.",

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year = "2023",

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doi = "10.1016/j.scr.2022.102990",

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T1 - CRISPR/Cas9 mediated approach to generate YAP-depleted human embryonic stem cell line (MUSIe002-A-1)

AU - Khampang, Sujittra

AU - Jiamvoraphong, Nittaya

AU - Laowtammathron, Chuti

AU - Lorthongpanich, Chanchao

AU - Klaihmon, Phatchanat

AU - Srisook, Pimonwan

AU - Kang, Xing

AU - Luanpitpong, Sudjit

AU - U-pratya, Yaowalak

AU - Issaragrisil, Surapol

PY - 2023/2

Y1 - 2023/2

N2 - Yes-associated protein (YAP), an important effector protein of the Hippo signaling pathway, acts as a molecular switch in controlling cell proliferation and apoptosis. In this study, a YAP-targeted isogenic sub-clone of the MUSIe002-A was generated, designated as MUSIe002-A-1. The MUSIe002-1 cell line had normal pluripotent stem cell characteristics and karyotype. Its ability to differentiate into three germ layers was confirmed. As reduction of YAP does not disturb the pluripotency of hESCs, this cell line serves as a valuable model to extrapolate the functional role of YAP in stem cell biology and its applications.

AB - Yes-associated protein (YAP), an important effector protein of the Hippo signaling pathway, acts as a molecular switch in controlling cell proliferation and apoptosis. In this study, a YAP-targeted isogenic sub-clone of the MUSIe002-A was generated, designated as MUSIe002-A-1. The MUSIe002-1 cell line had normal pluripotent stem cell characteristics and karyotype. Its ability to differentiate into three germ layers was confirmed. As reduction of YAP does not disturb the pluripotency of hESCs, this cell line serves as a valuable model to extrapolate the functional role of YAP in stem cell biology and its applications.

UR - https://www.scopus.com/pages/publications/85144418032

U2 - 10.1016/j.scr.2022.102990

DO - 10.1016/j.scr.2022.102990

M3 - Article

C2 - 36528979

AN - SCOPUS:85144418032

SN - 1873-5061

VL - 66

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 102990

ER -

CRISPR/Cas9 mediated approach to generate YAP-depleted human embryonic stem cell line (MUSIe002-A-1)

Abstract

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