Complex Interaction of α2-Adrenoceptor Binding Sites with Bovine Brain and Lung Extracts Containing Clonidine-Displacing Substance

D. Pinthong; D. A. Kendall; V. G. Wilson

doi:10.1196/annals.1304.026

Complex Interaction of α₂-Adrenoceptor Binding Sites with Bovine Brain and Lung Extracts Containing Clonidine-Displacing Substance

D. Pinthong
, D. A. Kendall
, V. G. Wilson

Nottingham University Hospitals NHS Trust

Research output: Contribution to journal › Article › peer-review

Abstract

Previous studies have established that methanolic extracts of bovine brain and lung possess CDS activity with an agonist-like profile at α ₂-adrenoceptor binding sites (i.e., greater potency against [ ³H]-clonidine sites than against [³H]-RX-821001 sites). Following prolonged reflux in ethylacetate, monovalent cations and histamine were removed from the lung extract, and the resulting extract exhibited similar potency against both radioligands, indicative of an antagonist-like profile. These observations help explain the absence of biologic activity at α ₂-adrenoceptors in the methanolic extracts as reported in our companion paper.

Original language	English
Pages (from-to)	216-221
Number of pages	6
Journal	Annals of the New York Academy of Sciences
Volume	1009
DOIs	https://doi.org/10.1196/annals.1304.026
Publication status	Published - 2003
Externally published	Yes

Keywords

Clonidine-displacing substance
α-adrenoceptor binding sites

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10.1196/annals.1304.026

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title = "Complex Interaction of α2-Adrenoceptor Binding Sites with Bovine Brain and Lung Extracts Containing Clonidine-Displacing Substance",

abstract = "Previous studies have established that methanolic extracts of bovine brain and lung possess CDS activity with an agonist-like profile at α 2-adrenoceptor binding sites (i.e., greater potency against [ 3H]-clonidine sites than against [3H]-RX-821001 sites). Following prolonged reflux in ethylacetate, monovalent cations and histamine were removed from the lung extract, and the resulting extract exhibited similar potency against both radioligands, indicative of an antagonist-like profile. These observations help explain the absence of biologic activity at α 2-adrenoceptors in the methanolic extracts as reported in our companion paper.",

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AU - Wilson, V. G.

PY - 2003

Y1 - 2003

N2 - Previous studies have established that methanolic extracts of bovine brain and lung possess CDS activity with an agonist-like profile at α 2-adrenoceptor binding sites (i.e., greater potency against [ 3H]-clonidine sites than against [3H]-RX-821001 sites). Following prolonged reflux in ethylacetate, monovalent cations and histamine were removed from the lung extract, and the resulting extract exhibited similar potency against both radioligands, indicative of an antagonist-like profile. These observations help explain the absence of biologic activity at α 2-adrenoceptors in the methanolic extracts as reported in our companion paper.

AB - Previous studies have established that methanolic extracts of bovine brain and lung possess CDS activity with an agonist-like profile at α 2-adrenoceptor binding sites (i.e., greater potency against [ 3H]-clonidine sites than against [3H]-RX-821001 sites). Following prolonged reflux in ethylacetate, monovalent cations and histamine were removed from the lung extract, and the resulting extract exhibited similar potency against both radioligands, indicative of an antagonist-like profile. These observations help explain the absence of biologic activity at α 2-adrenoceptors in the methanolic extracts as reported in our companion paper.

KW - Clonidine-displacing substance

KW - α-adrenoceptor binding sites

UR - https://www.scopus.com/pages/publications/1342326706

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SN - 0077-8923

VL - 1009

SP - 216

EP - 221

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

ER -

Complex Interaction of α₂-Adrenoceptor Binding Sites with Bovine Brain and Lung Extracts Containing Clonidine-Displacing Substance

Abstract

Keywords

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