Comparison of base inclinations for left‐handed DNA and RNA

Chartchai Krittanai; Hunseung Kang; Xiaokui Jin; Jeannine H. Riazance‐Lawrence; W. Johnson Curtis

doi:10.1002/bspy.350010403

Comparison of base inclinations for left‐handed DNA and RNA

Chartchai Krittanai
, Hunseung Kang
, Xiaokui Jin
, Jeannine H. Riazance‐Lawrence
, W. Johnson Curtis

Oregon State University

Research output: Contribution to journal › Article › peer-review

Abstract

The Z'‐form of poly(dG‐m⁵dC) · poly(dG‐m⁵dC) in 85% ethanol/MOPS and the Z‐form poly(rG‐rC) · poly(rG‐rC) in 4.8 M NaClO₄, 20% ethanol are studied by flow linear dichroism. Simulataneous analysis of the isotropic absorption and linear dichroism data yields the angle of inclination of the base normal relative to the helix axis, and the orientation of the axis around which the bases incline. For the Z'‐form of poly(dG‐m⁵dC) · poly(dG‐m⁵dC) the inclination angles are 31.3° for guanine, and 27.9° for cytosine; for the Z‐form of poly(rG‐rC) · poly(rG‐rC) they are 25.0° for guanine, and 23.3° for cytosine. The inclination angles for the Z'‐form DNA are similar to those for the Z‐form, and the angles for Z‐form RNA are somewhat smaller. The axes of inclination for guanine and cytosine are similar in all three left‐handed forms. © 1995 John Wiley & Sons, Inc.

Original language	English
Pages (from-to)	247-254
Number of pages	8
Journal	Biospectroscopy
Volume	1
Issue number	4
DOIs	https://doi.org/10.1002/bspy.350010403
Publication status	Published - 1995
Externally published	Yes

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title = "Comparison of base inclinations for left‐handed DNA and RNA",

abstract = "The Z'‐form of poly(dG‐m5dC) · poly(dG‐m5dC) in 85\% ethanol/MOPS and the Z‐form poly(rG‐rC) · poly(rG‐rC) in 4.8 M NaClO4, 20\% ethanol are studied by flow linear dichroism. Simulataneous analysis of the isotropic absorption and linear dichroism data yields the angle of inclination of the base normal relative to the helix axis, and the orientation of the axis around which the bases incline. For the Z'‐form of poly(dG‐m5dC) · poly(dG‐m5dC) the inclination angles are 31.3° for guanine, and 27.9° for cytosine; for the Z‐form of poly(rG‐rC) · poly(rG‐rC) they are 25.0° for guanine, and 23.3° for cytosine. The inclination angles for the Z'‐form DNA are similar to those for the Z‐form, and the angles for Z‐form RNA are somewhat smaller. The axes of inclination for guanine and cytosine are similar in all three left‐handed forms. {\textcopyright} 1995 John Wiley \& Sons, Inc.",

author = "Chartchai Krittanai and Hunseung Kang and Xiaokui Jin and Riazance‐Lawrence, \{Jeannine H.\} and Curtis, \{W. Johnson\}",

year = "1995",

doi = "10.1002/bspy.350010403",

language = "English",

volume = "1",

pages = "247--254",

journal = "Biospectroscopy",

issn = "1075-4261",

publisher = "John Wiley and Sons Inc.",

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TY - JOUR

T1 - Comparison of base inclinations for left‐handed DNA and RNA

AU - Krittanai, Chartchai

AU - Kang, Hunseung

AU - Jin, Xiaokui

AU - Riazance‐Lawrence, Jeannine H.

AU - Curtis, W. Johnson

PY - 1995

Y1 - 1995

N2 - The Z'‐form of poly(dG‐m5dC) · poly(dG‐m5dC) in 85% ethanol/MOPS and the Z‐form poly(rG‐rC) · poly(rG‐rC) in 4.8 M NaClO4, 20% ethanol are studied by flow linear dichroism. Simulataneous analysis of the isotropic absorption and linear dichroism data yields the angle of inclination of the base normal relative to the helix axis, and the orientation of the axis around which the bases incline. For the Z'‐form of poly(dG‐m5dC) · poly(dG‐m5dC) the inclination angles are 31.3° for guanine, and 27.9° for cytosine; for the Z‐form of poly(rG‐rC) · poly(rG‐rC) they are 25.0° for guanine, and 23.3° for cytosine. The inclination angles for the Z'‐form DNA are similar to those for the Z‐form, and the angles for Z‐form RNA are somewhat smaller. The axes of inclination for guanine and cytosine are similar in all three left‐handed forms. © 1995 John Wiley & Sons, Inc.

AB - The Z'‐form of poly(dG‐m5dC) · poly(dG‐m5dC) in 85% ethanol/MOPS and the Z‐form poly(rG‐rC) · poly(rG‐rC) in 4.8 M NaClO4, 20% ethanol are studied by flow linear dichroism. Simulataneous analysis of the isotropic absorption and linear dichroism data yields the angle of inclination of the base normal relative to the helix axis, and the orientation of the axis around which the bases incline. For the Z'‐form of poly(dG‐m5dC) · poly(dG‐m5dC) the inclination angles are 31.3° for guanine, and 27.9° for cytosine; for the Z‐form of poly(rG‐rC) · poly(rG‐rC) they are 25.0° for guanine, and 23.3° for cytosine. The inclination angles for the Z'‐form DNA are similar to those for the Z‐form, and the angles for Z‐form RNA are somewhat smaller. The axes of inclination for guanine and cytosine are similar in all three left‐handed forms. © 1995 John Wiley & Sons, Inc.

UR - https://www.scopus.com/pages/publications/84984270066

U2 - 10.1002/bspy.350010403

DO - 10.1002/bspy.350010403

M3 - Article

AN - SCOPUS:84984270066

SN - 1075-4261

VL - 1

SP - 247

EP - 254

JO - Biospectroscopy

JF - Biospectroscopy

IS - 4

ER -

Comparison of base inclinations for left‐handed DNA and RNA

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