TY - JOUR
T1 - Comparative genomic analysis of Streptococcus suis sequence type 105 and development of a PCR diagnostic tool
AU - Boueroy, Parichart
AU - Chopjitt, Peechanika
AU - Borthong, Jednipit
AU - Wongsurawat, Thidathip
AU - Jenjaroenpun, Piroon
AU - Duangjanchot, Rapeephan
AU - Saiboonjan, Bhanubong
AU - Hatrongjit, Rujirat
AU - Kerdsin, Anusak
N1 - Publisher Copyright:
© 2025 Boueroy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2025/5
Y1 - 2025/5
N2 - Streptococcus suis serotype 14 is the second most prevalent serotype after serotype 2, and is highly prevalent in Southeast Asia. Among the serotype 14 strains, sequence type (ST) 105 is found in humans and pigs. We analysed the genome sequences of S. suis ST105 to identify unique sequences to develop a multiplex PCR (mPCR) -gel electrophoresis and mPCR-lateral flows trip (LFS) for epidemiological purposes. The ST105 genome was closely related to the ST1 genomes. All ST105 of Thai and Vietnamese strains were highly homologous. Of the 1818 genes found in all compared genomes, 36 unique sequences were detected only in the ST105 strain. Of these, two unique sequences encoding hypothetical proteins were selected as PCR targets. Only S. suis ST105 strains were positive for both mPCRs. mPCR-LFS had fewer complications, lower costs, and less time for testing, than those of mPCR-gel electrophoresis. This comparative genomic study demonstrates the usefulness of identifying unique sequences of ST105 S. suis. These unique sequences could be used to develop diagnostic or screening tools, such as PCR, for the detection of specific strains or clones for epidemiological purposes.
AB - Streptococcus suis serotype 14 is the second most prevalent serotype after serotype 2, and is highly prevalent in Southeast Asia. Among the serotype 14 strains, sequence type (ST) 105 is found in humans and pigs. We analysed the genome sequences of S. suis ST105 to identify unique sequences to develop a multiplex PCR (mPCR) -gel electrophoresis and mPCR-lateral flows trip (LFS) for epidemiological purposes. The ST105 genome was closely related to the ST1 genomes. All ST105 of Thai and Vietnamese strains were highly homologous. Of the 1818 genes found in all compared genomes, 36 unique sequences were detected only in the ST105 strain. Of these, two unique sequences encoding hypothetical proteins were selected as PCR targets. Only S. suis ST105 strains were positive for both mPCRs. mPCR-LFS had fewer complications, lower costs, and less time for testing, than those of mPCR-gel electrophoresis. This comparative genomic study demonstrates the usefulness of identifying unique sequences of ST105 S. suis. These unique sequences could be used to develop diagnostic or screening tools, such as PCR, for the detection of specific strains or clones for epidemiological purposes.
UR - https://www.scopus.com/pages/publications/105005995888
U2 - 10.1371/journal.pone.0324636
DO - 10.1371/journal.pone.0324636
M3 - Article
C2 - 40392831
AN - SCOPUS:105005995888
SN - 1932-6203
VL - 20
JO - PLoS ONE
JF - PLoS ONE
IS - 5 May
M1 - e0324636
ER -