Characteristics of acid- and pepsin-soluble collagens from scale of seabass (Lates calcarifer)

Sira Chuaychan; Soottawat Benjakul; Hideki Kishimura

doi:10.1016/j.lwt.2015.03.002

Characteristics of acid- and pepsin-soluble collagens from scale of seabass (Lates calcarifer)

Sira Chuaychan
, Soottawat Benjakul
, Hideki Kishimura

Prince of Songkla University
Hokkaido University

Research output: Contribution to journal › Article › peer-review

119 Citations (Scopus)

Abstract

Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) from seabass (Lates calcarifer) scale were characterised. Yields of ASC and PSC were 0.38 and 1.06% (based on dry weight), respectively. Both ASC and PSC were identified to be type I collagen, which consisted of α₁ and α₂ chains. β component was also found in both collagens. ASC and PSC contained glycine as the major amino acid and had high imino acid content (193-198 residues/1000 residues). T_max of ASC and PSC from seabass scale were 38.17°C and 39.32°C, respectively. FTIR spectra indicated that triple helical structure of resulting PSC was not disrupted by pepsin digestion. ASC and PSC from seabass scale exhibited high solubility in very acidic pH range (pH 2-4). Therefore, seabass scale could be an alternative source of collagen and the characteristics of collagens were slightly affected by extraction process used.

Original language	English
Pages (from-to)	71-76
Number of pages	6
Journal	LWT
Volume	63
Issue number	1
DOIs	https://doi.org/10.1016/j.lwt.2015.03.002
Publication status	Published - 1 Sept 2015
Externally published	Yes

Keywords

Acid soluble collagen
Pepsin soluble collagen
Scales
Seabass

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10.1016/j.lwt.2015.03.002

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title = "Characteristics of acid- and pepsin-soluble collagens from scale of seabass (Lates calcarifer)",

abstract = "Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) from seabass (Lates calcarifer) scale were characterised. Yields of ASC and PSC were 0.38 and 1.06\% (based on dry weight), respectively. Both ASC and PSC were identified to be type I collagen, which consisted of α1 and α2 chains. β component was also found in both collagens. ASC and PSC contained glycine as the major amino acid and had high imino acid content (193-198 residues/1000 residues). Tmax of ASC and PSC from seabass scale were 38.17°C and 39.32°C, respectively. FTIR spectra indicated that triple helical structure of resulting PSC was not disrupted by pepsin digestion. ASC and PSC from seabass scale exhibited high solubility in very acidic pH range (pH 2-4). Therefore, seabass scale could be an alternative source of collagen and the characteristics of collagens were slightly affected by extraction process used.",

keywords = "Acid soluble collagen, Pepsin soluble collagen, Scales, Seabass",

author = "Sira Chuaychan and Soottawat Benjakul and Hideki Kishimura",

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year = "2015",

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T1 - Characteristics of acid- and pepsin-soluble collagens from scale of seabass (Lates calcarifer)

AU - Chuaychan, Sira

AU - Benjakul, Soottawat

AU - Kishimura, Hideki

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) from seabass (Lates calcarifer) scale were characterised. Yields of ASC and PSC were 0.38 and 1.06% (based on dry weight), respectively. Both ASC and PSC were identified to be type I collagen, which consisted of α1 and α2 chains. β component was also found in both collagens. ASC and PSC contained glycine as the major amino acid and had high imino acid content (193-198 residues/1000 residues). Tmax of ASC and PSC from seabass scale were 38.17°C and 39.32°C, respectively. FTIR spectra indicated that triple helical structure of resulting PSC was not disrupted by pepsin digestion. ASC and PSC from seabass scale exhibited high solubility in very acidic pH range (pH 2-4). Therefore, seabass scale could be an alternative source of collagen and the characteristics of collagens were slightly affected by extraction process used.

AB - Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) from seabass (Lates calcarifer) scale were characterised. Yields of ASC and PSC were 0.38 and 1.06% (based on dry weight), respectively. Both ASC and PSC were identified to be type I collagen, which consisted of α1 and α2 chains. β component was also found in both collagens. ASC and PSC contained glycine as the major amino acid and had high imino acid content (193-198 residues/1000 residues). Tmax of ASC and PSC from seabass scale were 38.17°C and 39.32°C, respectively. FTIR spectra indicated that triple helical structure of resulting PSC was not disrupted by pepsin digestion. ASC and PSC from seabass scale exhibited high solubility in very acidic pH range (pH 2-4). Therefore, seabass scale could be an alternative source of collagen and the characteristics of collagens were slightly affected by extraction process used.

KW - Acid soluble collagen

KW - Pepsin soluble collagen

KW - Scales

KW - Seabass

UR - https://www.scopus.com/pages/publications/84928696133

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DO - 10.1016/j.lwt.2015.03.002

M3 - Article

AN - SCOPUS:84928696133

SN - 0023-6438

VL - 63

SP - 71

EP - 76

JO - LWT

JF - LWT

IS - 1

ER -

Characteristics of acid- and pepsin-soluble collagens from scale of seabass (Lates calcarifer)

Abstract

Keywords

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