Astakine1 forms protein complex in plasma

Ratchanok Sirikharin; Chadanat Noonin; Kingkamon Junkunlo; Kenneth Söderhäll; Irene Söderhäll

doi:10.1016/j.fsi.2019.08.063

Astakine1 forms protein complex in plasma

Ratchanok Sirikharin
, Chadanat Noonin
, Kingkamon Junkunlo
, Kenneth Söderhäll
, Irene Söderhäll

Uppsala University

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Astakine 1 is a small cytokine-like peptide which is directly involved in hematopoiesis in crustaceans. Astakines are present in many different invertebrate groups primarily in arthropods. In this study we found that astakine1 was present as a high molecular weight (HMW) complex in plasma. It is known that calcium concentration are fluctuating in several crustaceans especially during the molting process. This HMW-complex was formed under low calcium concentrations in plasma and could be partially reversed provided calcium was added. The biological function of the naïve astakine1 and that in the HMW complex was about the same, but if the protein is to be isolated or studied for its function it is important to know about this property of astakine1 which may previously have hampered isolation and functional studies in other animals than freshwater crayfish.

Original language	English
Pages (from-to)	66-71
Number of pages	6
Journal	Fish and Shellfish Immunology
Volume	94
DOIs	https://doi.org/10.1016/j.fsi.2019.08.063
Publication status	Published - Nov 2019
Externally published	Yes

Keywords

Cytokine
Hematopoiesis
Pacifastacus leniusculus
Plasma protein

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10.1016/j.fsi.2019.08.063

Cite this

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title = "Astakine1 forms protein complex in plasma",

abstract = "Astakine 1 is a small cytokine-like peptide which is directly involved in hematopoiesis in crustaceans. Astakines are present in many different invertebrate groups primarily in arthropods. In this study we found that astakine1 was present as a high molecular weight (HMW) complex in plasma. It is known that calcium concentration are fluctuating in several crustaceans especially during the molting process. This HMW-complex was formed under low calcium concentrations in plasma and could be partially reversed provided calcium was added. The biological function of the na{\"i}ve astakine1 and that in the HMW complex was about the same, but if the protein is to be isolated or studied for its function it is important to know about this property of astakine1 which may previously have hampered isolation and functional studies in other animals than freshwater crayfish.",

keywords = "Cytokine, Hematopoiesis, Pacifastacus leniusculus, Plasma protein",

author = "Ratchanok Sirikharin and Chadanat Noonin and Kingkamon Junkunlo and Kenneth S{\"o}derh{\"a}ll and Irene S{\"o}derh{\"a}ll",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

year = "2019",

month = nov,

doi = "10.1016/j.fsi.2019.08.063",

language = "English",

volume = "94",

pages = "66--71",

journal = "Fish and Shellfish Immunology",

issn = "1050-4648",

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TY - JOUR

T1 - Astakine1 forms protein complex in plasma

AU - Sirikharin, Ratchanok

AU - Noonin, Chadanat

AU - Junkunlo, Kingkamon

AU - Söderhäll, Kenneth

AU - Söderhäll, Irene

PY - 2019/11

Y1 - 2019/11

N2 - Astakine 1 is a small cytokine-like peptide which is directly involved in hematopoiesis in crustaceans. Astakines are present in many different invertebrate groups primarily in arthropods. In this study we found that astakine1 was present as a high molecular weight (HMW) complex in plasma. It is known that calcium concentration are fluctuating in several crustaceans especially during the molting process. This HMW-complex was formed under low calcium concentrations in plasma and could be partially reversed provided calcium was added. The biological function of the naïve astakine1 and that in the HMW complex was about the same, but if the protein is to be isolated or studied for its function it is important to know about this property of astakine1 which may previously have hampered isolation and functional studies in other animals than freshwater crayfish.

AB - Astakine 1 is a small cytokine-like peptide which is directly involved in hematopoiesis in crustaceans. Astakines are present in many different invertebrate groups primarily in arthropods. In this study we found that astakine1 was present as a high molecular weight (HMW) complex in plasma. It is known that calcium concentration are fluctuating in several crustaceans especially during the molting process. This HMW-complex was formed under low calcium concentrations in plasma and could be partially reversed provided calcium was added. The biological function of the naïve astakine1 and that in the HMW complex was about the same, but if the protein is to be isolated or studied for its function it is important to know about this property of astakine1 which may previously have hampered isolation and functional studies in other animals than freshwater crayfish.

KW - Cytokine

KW - Hematopoiesis

KW - Pacifastacus leniusculus

KW - Plasma protein

UR - https://www.scopus.com/pages/publications/85071537623

U2 - 10.1016/j.fsi.2019.08.063

DO - 10.1016/j.fsi.2019.08.063

M3 - Article

C2 - 31465872

AN - SCOPUS:85071537623

SN - 1050-4648

VL - 94

SP - 66

EP - 71

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

ER -

Astakine1 forms protein complex in plasma

Abstract

Keywords

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